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Coral microbiome preservation and extraction method comparison of samples collected in March and August 2018-raw data

Metadata Updated: October 8, 2025

The files in this this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) are the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 90 samples of tropical and cold-water corals, as well as sequence files from a mock community and extraction blanks for the kits used for DNA extraction. The mock community was sequenced in order to assess any biases in the sequencing technology, while extraction blanks were sequenced in order to identify any contaminants in the DNA extraction kits. The tropical coral samples (three species) were collected by permit (#FKNMS-2017-064) in March 2018 from a nursery in the Florida Keys National Marine Sanctuary. The cold-water coral samples (two species) were collected in August 2018 from two locations in the Atlantic Ocean.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

Downloads & Resources

Dates

Metadata Created Date September 13, 2025
Metadata Updated Date October 8, 2025

Metadata Source

Harvested from DOI USGS DCAT-US

Additional Metadata

Resource Type Dataset
Metadata Created Date September 13, 2025
Metadata Updated Date October 8, 2025
Publisher U.S. Geological Survey
Maintainer
Identifier http://datainventory.doi.gov/id/dataset/usgs-ba147df0-0de1-4f37-92f6-2712c0e21547
Data Last Modified 2021-09-23T00:00:00Z
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id e9c91bc1-47cd-4792-950c-325d9aba452f
Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
Harvest Source Title DOI USGS DCAT-US
Metadata Type geospatial
Old Spatial -81.45441, 24.661751, -75.16639, 34.9410185
Source Datajson Identifier True
Source Hash fc31d4b9d69c646c3e3ba206f522fd581d7abd918e1b7f726f263d7fe477c757
Source Schema Version 1.1
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