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    • Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples

    Metadata Updated: July 6, 2024

    We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date June 1, 2023
    Metadata Updated Date July 6, 2024

    Metadata Source

    Harvested from DOI EDI

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date June 1, 2023
    Metadata Updated Date July 6, 2024
    Publisher U.S. Geological Survey
    Maintainer
    @Id http://datainventory.doi.gov/id/dataset/3af443f39dca80a8d75bceb5a3c7afa6
    Identifier USGS:594bedc5e4b062508e3850f0
    Data Last Modified 20200826
    Category geospatial
    Public Access Level public
    Bureau Code 010:12
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://datainventory.doi.gov/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 1aed6073-4ff6-4d4f-9f03-c0f45dd23426
    Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
    Harvest Source Title DOI EDI
    Metadata Type geospatial
    Old Spatial -125.0683593736,33.541029991959,-103.97460937444,48.777624430256
    Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
    Source Datajson Identifier True
    Source Hash ee6617bc3e991c216a7aafb0625ca4e356b71509e89ad105e3d795241239a6bd
    Source Schema Version 1.1
    Spatial {"type": "Polygon", "coordinates": -125.0683593736, 33.541029991959, -125.0683593736, 48.777624430256, -103.97460937444, 48.777624430256, -103.97460937444, 33.541029991959, -125.0683593736, 33.541029991959}

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