Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Adam J Sepulveda",
"@type": "vcard:Contact",
"hasEmail": "mailto:asepulveda@usgs.gov"
}
|
| description | We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. |
| distribution |
[
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"@type": "dcat:Distribution",
"title": "Digital Data",
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"accessURL": "https://doi.org/10.5066/F7C53JDW",
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"title": "Original Metadata",
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_594bedc5e4b062508e3850f0 |
| keyword |
[
"California",
"East Gallatin River",
"Idaho",
"Montana",
"PCR",
"Proliferative Kidney Disease",
"Sypes Canyon",
"Tetracapsuloides bryosalmonae",
"USGS:594bedc5e4b062508e3850f0",
"Wyoming",
"biota",
"environmental DNA",
"fish",
"molecular biology",
"parasites"
]
|
| modified | 2020-08-26T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -125.0683593736, 33.541029991959, -103.97460937444, 48.777624430256 |
| theme |
[
"geospatial"
]
|
| title | Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples |
