Comparison of Transcriptomic Points of Departure (tPOD) with Apical Endpoints in Developing Zebrafish Exposed to PFOS, PFOA, or PFHxS

Comparison of Transcriptomic Points of Departure (tPOD) with Apical Endpoints in Developing Zebrafish Exposed to PFOS, PFOA, or PFHxS. Provides full name, abbreviation, and identifiers for the PFAS tested in this study Provides the 14 zebrafish developmental exposure windows, and timepoints of morphological and behavior endpoint characterization, and RNA collection for transcriptomics; Figure 1 in manuscript Exposure verification data for the three PFAS and all exposure windows; Table 1 and Supplementary Table 1 in manuscript Developmental toxicity data for the three PFAS, DMSO, and Chlorpyrifos; Figures 2, 3, 5, and Supplementary Figures S1, S2, S5, S6 in manuscript Data for length measurements of fish at 96, 120, and 144 hpf exposed to DMSO, PFOS, PFOA, or PFHxS from 6, 24, 48, or 96 hpf; Figure 2 and Supplmentary Figures S3, S7 in manuscript Distance moved (mm) in a 120-hpf-behavior assay with two alternating dark-light cycles for fish exposed to DMSO, PFOS, PFOA, or PFHxS from 6, 24, 48 or 96 hpf; Figures 2, 3, 5 and Supplementary Figures S4, S8 in manuscript Statistical summary of developmental toxicity data for the three PFAS when exposure started at 6, 24, 48, or 96 hpf; Figure 2 in manuscript Benchmark concentrations derived for mortality, swim bladder (SB) inflation, length, and behavior, and associated upper (BMCU) and lower (BMCL) 95% confidence limits, with BMCLs designated as the aPODs; Figures 3 and 5 in manuscript Links to the public repositories where raw RNAseq data (FASTQ format), processed and normalized counts, and associated sample meta-data can be obtained; Figures 4 , 5, 6 in manuscript Trancriptomic-based points of departure and concentration responsive gene counts for developing zebrafish after seven PFAS exposure windows, and tPODs from previous published Daphnia magna and fathead minnow literature; Table 2 and Figures 4,5, and 6 in manuscript Parameters used to query the ECOTOX knowledgebase for zebrafish records for the tested PFAS; Figure 6 and Supplementary Methods SM.2. in manuscript Full listing of ECOTOX records retrieved using search parameters defined in ECOTOX-search including citations for PFOS Full listing of ECOTOX records retrieved using search parameters defined in ECOTOX-search including citations for PFOA Full listing of ECOTOX records retrieved using search parameters defined in ECOTOX-search including citations for PFHxS. This dataset is associated with the following publication: Shankar, P., M. Hazemi, K. Mattingly, M.J. See, M. Bell, M. Ellman, J. Cavallin, S. Lasee, B. Blackwell, S. Vliet, A. Biales, C. LaLone, and D. Villeneuve. Comparison of transcriptomic points of departure with apical endpoints in developing zebrafish exposed to PFOS, PFOA, or PFHxS. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 206(1): 134-146, (2025).

[
  "020:00"
]

{
  "fn": "Daniel Villeneuve",
  "hasEmail": "mailto:villeneuve.dan@epa.gov"
}

[
  {
    "title": "Zebrafish PFAS tPOD study_Science Hub_2025FEB03_MH_Final.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1531975/Zebrafish%20PFAS%20tPOD%20study_Science%20Hub_2025FEB03_MH_Final.xlsx"
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[
  "high throughput transcriptomics",
  "new approach methodologies",
  "per and polyfluoroalkyl substances (PFAS)",
  "zebra fish"
]

[
  "020:000"
]

{
  "name": "U.S. Environmental Protection Agency",
  "subOrganizationOf": {
    "name": "U.S. Government"
  }
}

[
  "https://doi.org/10.1093/toxsci/kfaf047"
]

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