Data include total nitrogen and carbon, carbon species, total metal, and particle-size analyses of soft streambed from the Black Creek basin, Allen County, Indiana that was collected in July 2019. These data will be used in a sediment-source mixing model in order to attribute the proportional contribution of individual sources to suspended sediment in the basin. Source samples were identified as one of five land-cover types: cropland (corn, soybean, hay, and wheat; sites included a mix of conventional and conservation tillage), active horse pasture, roads, preserved forest, and streambanks along agricultural and non-agricultural channels. Source data were collected in August 2017 (Williamson and others, 2020). Williamson, T.N, Hardebeck, M.J, and Martin, G.R, 2020, Chemical and physical data for sediment source fingerprinting of suspended and bottom sediment in Black Creek, Indiana, tributary to the Maumee River and western Lake Erie - Source samples: U.S. Geological Survey data release, https://doi.org/10.5066/P9W28HF0.