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Changes in gene expression in Arabidopsis in response to nano CeO2 and nano TiO2

Metadata Updated: May 2, 2021

  • Changes in tissue transcriptomes and productivity of Arabidopsis thaliana were investigated during exposure of plants to two widely used engineered metal oxide nanoparticles, titanium dioxide (nano-titanium) and cerium dioxide (nano-cerium). Microarray analyses confirmed that exposure to either nanoparticle altered the transcriptomes of rosette leaves and roots, with comparatively larger numbers of differentially expressed genes (DEGs) found under nano-titania exposure. Nano-titania induced more DEGs in rosette leaves, whereas roots had more DEGs under nano-ceria exposure. MapMan analyses indicated that while nano-titania up-regulated overall metabolism metabolism in both tissues, metabolic processes under nano-ceria remained mostly unchanged. Gene enrichment analysis indicated that both nanoparticles mainly enriched ontology groups such as responses to stress (abiotic and biotic), and defense responses (pathogens), and responses to endogenous stimuli (hormones). Nano-titania specifically induced genes associated with photosynthesis, whereas nano-ceria induced expression of genes related to activating transcription factors, most notably those belonging to the ethylene responsive element binding protein family. Interestingly, there were also increased numbers of rosette leaves and plant biomass under nano-ceria exposure, but not under nano-titania. Other transcriptomic responses did not clearly relate to responses observed at the organism level. This may be due to functional and genomic redundancy in Arabidopsis, which may mask expression of morphological changes, despite discernable responses at the transcriptome level. Additionally, transcriptomic changes often relate with transgenerational phenotypic development, hence it may be productive to direct further experimental work to integrate high-throughput genomic results with longer-term changes in subsequent generations.

This dataset is associated with the following publication: Tumburu, L., C. Andersen, P.T. Rygiewicz, and J. Reichman. Molecular and physiological responses to titanium dioxide and cerium oxide nanoparticles in arabidopsis. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(1): 71-82, (2017).

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Public: This dataset is intended for public access and use. License: See this page for license information.

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Metadata Created Date November 12, 2020
Metadata Updated Date May 2, 2021

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date May 2, 2021
Publisher U.S. EPA Office of Research and Development (ORD)
Data Last Modified 2018-08-27
Public Access Level public
Bureau Code 020:00
Schema Version
Harvest Object Id 1001daea-6158-4bcb-8a03-dfc79f52b53b
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
Program Code 020:095
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents
Source Datajson Identifier True
Source Hash fa39290b42c67f6a616d626a5abb24a138a1c1a0
Source Schema Version 1.1

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