The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. A standardized reef fish survey is conducted in the U.S. Caribbean every 2nd or 3rd year with the objective of determining the relative abundance of reef fish on the shelf waters of Puerto Rico and the U.S. Virgin Islands. This survey uses video cameras, fish traps and vertical line gear, with approximately 200 video cameras, 200 vertical line and 100 traps conducted. The camera array consists of four housings positioned orthogonally and center mounted at a height of 51 cm above the bottom of the array. Each housing contains a pair of black-and-white Videre stereo cameras along with a color mpeg camera. Sampling of reef sites with video cameras occurs only during daylight hours, with the first gear deployment one hour after sunrise and the last gear retrieval one hour prior to sunset. Video arrays are soaked for 35 minutes. At sites selected for fish sampling, a chevron (or arrow) fish trap or vertical line is used to capture fish for biological samples. The chevron fish trap is constructed with 1.5-inch vinyl-clad mesh. In its greatest dimensions, the trap is 1.76 m in length, 1.52 m in width and 0.61 m in depth. A 0.4 m by 0.29 m blow out panel is placed on one side and kept closed using 7-day magnesium releases. The fish trap soaks for one hour and is baited with squid. The vertical line consists of a mainline with 10 gangions attached that is either deployed or attached to the vessel. One 8/0, 11/0 or 15/0 circle hook is attached to each gangion and baited with mackerel (Scomber scombrus). The mainline is soaked for five minutes. Most of the animals captured are measured, weighed, tagged and then released. Those individuals which are moribund or have expired are retained to collect biological data pertaining to the life history of these fishes. Habitat mapping is conducted using the SIMRAD ME70 multibeam echosounder. At each site hydrological data is collected using Conductivity Temperature Depth sensor (CTD).