This data release provides data for quantifying the aquatic export of total carbon in a cypress swamp within the Big Cypress National Preserve, and is part of a larger carbon budget study. The U.S. Geological Survey operated two continuous streamflow and water-quality monitoring stations within the Big Cypress National Preserve in southern Florida from September 2015 to October 2017. Site-specific regression models were developed using continuously measured specific conductance and concomitant discretely collected total carbon (dissolved organic carbon, dissolved inorganic carbon, and particulate carbon) samples to calculate continuous concentrations of total carbon. Total carbon loads were calculated by multiplying concentrations and streamflow, and the difference between the load calculations was used to determine the aquatic carbon export of the study area.