The Eastern Brown Pelican (Pelecanus occidentalis carolinensis) is a large-bodied seabird that nests in colonies of 10 to upwards of 5,000 pairs, on nearshore islands in subtropical and tropical North American waters. It breeds between March and August, laying 2–3 eggs and raising 1–2 chicks per year. The species is facultatively migratory during nonbreeding, with some individuals remaining resident and others leaving breeding areas. Pelicans forage in near- and offshore waters and capture schooling fish by plunge-diving. This dataset summarizes blood analyte values collected from Brown Pelican nestlings and breeding adults from 2013-2015 in the Northern Gulf of Mexico at 9 breeding colonies from Texas to the Florida Panhandle. Evaluated analytes included alanine aminotransferase, aspartate aminotransferase, creatinine phosphokinase, gamma glutamyl transferase, lactate dehydrogenase, amylase, bile acids, blood urea nitrogen, calcium, cholesterol, CO2, creatinine, glucose, lipase, phosphorus, potassium, sodium, total protein, triglycerides, and uric acid. Lipoprotein analysis included high-density and very low-density lipoprotein cholesterol. The concentration of all white blood cells and the concentration of heterophils, lymphocytes, monocytes, eosinophils, and basophils were also measured.