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Biological variables and liver and testes transcript abundance from smallmouth bass sampled in the Lake Erie drainage, Pennsylvania (2016)

Published by U.S. Geological Survey | Department of the Interior | Catalog Last Checked: August 01, 2026 at 03:54 AM | Dataset Last Updated: August 05, 2022 at 12:00 AM
In 2016, smallmouth bass were collected from three sites in the Lake Erie drainage in Erie County, Pennsylvania and an out-of-basin site in the Allegheny River drainage in order to assess reproductive health and identify endocrine disruption responses. Pieces of liver and gonad were taken in Z-Fix and RNALater for histology and transcript abundance analysis. A landscape analysis was conducted for the immediate and upstream catchments with 2016 land cover data. Correlations between biological variables and transcripts were made in addition to differences between sites. The results showed that males had biological variables that correlated with transcripts associated with endocrine disruption and a high rate of testicular oocytes and plasma vitellogenin (biomarkers of estrogenic contaminant exposure).

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