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Bacterial and archael 16S rRNA sequences and taxonomic summary tables for biofilm samples from the bio-reactors

Metadata Updated: November 12, 2020

A biofilm anode acclimated with acetate, acetate+methane, and methane growth media for over three years produced a steady current density of 1.6-2.3 mA/m^2 in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. A fluorescence in situ hybridization (FISH) image clearly showed a biofilm of bacteria and archaea, supporting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs correlated linearly to the methane-gas concentration in the range of 10% to 99.97% (R^2 ≥ 0.99) when the measurement was sustained for at least 50 min. Thus, cumulative coulombs over 50 min. could be used to quantify the methane concentration in gas samples.

This dataset is associated with the following publication: Gao, Y., H. Ryu, B. Rittmann, A. Hussain, and H. Lee. Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer. Bioresource Technology. Elsevier Online, New York, NY, USA, 241: 979-984, (2017).

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Public: This dataset is intended for public access and use. License: See this page for license information.

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Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher U.S. EPA Office of Research and Development (ORD)
Data Last Modified 2017-09-06
Public Access Level public
Bureau Code 020:00
Schema Version
Harvest Object Id c0c952bc-be8b-4623-be85-bdeb559eb209
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
Program Code 020:096
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents
Source Datajson Identifier True
Source Hash 961fc888695716a9bff5c501191c042ce9b26a7a
Source Schema Version 1.1

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