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    • AFSC/RACE/EcoFOCI - Genetics analyses performed in support of FOCI assessment surveys and ecosystem observations in the Bering Sea and the Gulf of Alaska 2009 - 2010

    Metadata Updated: April 1, 2024

    The datasets contain nucleotide sequence data that is in text format and each line of sequence has a unique sample code (combination of letters and numbers).

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

    Downloads & Resources

    Dates

    Metadata Date February 29, 2024
    Metadata Created Date October 28, 2022
    Metadata Updated Date April 1, 2024
    Reference Date(s) 2014 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NMFS AFSC

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Date February 29, 2024
    Metadata Created Date October 28, 2022
    Metadata Updated Date April 1, 2024
    Reference Date(s) 2014 (publication)
    Responsible Party (Point of Contact, Custodian)
    Contact Email
    Guid gov.noaa.nmfs.inport:26576
    Access Constraints Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI - Genetics analyses performed in support of FOCI assessment surveys and ecosystem observations in the Bering Sea and the Gulf of Alaska 2009 - 2010 [Data Date Range], https://www.fisheries.noaa.gov/inport/item/26576., Access Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy., Use Constraints: Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
    Bbox East Long -150
    Bbox North Lat 60
    Bbox South Lat 50
    Bbox West Long -180
    Coupled Resource
    Frequency Of Update asNeeded
    Harvest Object Id adf5cb8a-021c-417a-b761-f19f0af6c52c
    Harvest Source Id 26a29bb9-50b0-47fd-920b-edc74aa6ec76
    Harvest Source Title NMFS AFSC
    Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
    Lineage PCR products from fish samples were sequenced using ABI Big Dye chemistry version 3.1 (Applied Biosystems, Inc) and run on an ABI 3730 automated sequencer. KB BASECALLER quality scores were generated using ABI data collection software version 3.0, and bidirectional sequences were aligned in the computer program SEQUENCHER version 4.9 (Gene Codes Corp.). Both directions were sequenced and aligned for reference specimen samples that showed intraspecific variation in RFLP banding patterns; otherwise, sequence from one direction was analyzed for PCR-RFLP assay development. Likewise, DNA sequence from one direction was analyzed for prey samples not assigned to one of the target fish groups (i.e. that could not be identified using the PCR-RFLP protocols), or were classified as unknown teleosts. Replicate DNA extractions of 12 prey items were sequenced in order to verify consistency of sequence data between replicates and to test for contamination from other prey species. Unique COI haplotypes from reference specimens were deposited in BOLD (DNA barcodes of marine fishes from the Northeast Pacific Ocean and Bering Sea, Proj. Code WXYZ http://www.boldsystems.org/). Sequences from samples that could not be identified using the PCR-RFLP protocols, or that were classified as unknown teleosts, were compared to the database of reference sequences in GenBank using the BLAST (Basic Local Alignment Search Tool) query algorithm.]
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-180.0, 50.0], [-150.0, 50.0], [-150.0, 60.0], [-180.0, 60.0], [-180.0, 50.0]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2009
    Temporal Extent End 2010

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