These acoustic Doppler current profiler (ADCP) data were collected and compiled to characterize the velocity patterns in channel of the Colorado River and in an adjacent zone of laterally recirculating flow (eddy) during both average flow conditions and during a controlled flood that occurred in March 2008. The objectives of the study were to measure changes in velocity and flow structure during changes in discharge associated with the controlled flood. These data were collected between March 4 and March 10, 2008 in a 1-mile study reach on the Colorado River within Grand Canyon National Park beginning 44.5 miles downstream from Lees Ferry, Arizona. These data were collected by the USGS Grand Canyon Monitoring and Research Center with cooperators from Northern Arizona University and funding provided by the Glen Canyon Dam Adaptive Management Program. All ADCP data were collected with an RD Instruments Workhorse Rio Grande with 600 kHz frequency. These data can be used to study streamflow velocity patterns in zones of recirculating flow, which can be used in predictions of sediment transport and can be used to develop, calibrate, and verify streamflow models.