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    • AbsorbanceQ App for Generating Absorbance Images from Brightfield Image Captures

    Metadata Updated: July 29, 2022

    This page hosts the AbsorbanceQ app which generates absorbance images from brightfield image captures. Absorbance microscopy can be used to make trypan blue cell viability measurements more quantitative and traceable by measuring the moles of trypan blue in each dead cell. AbsorbanceQ was written in MATLAB and can be downloaded as a .zip file. When unzipped, the program will be installed and can run as a standalone .exe file with no dependencies beyond the operating system. This website includes images that can be downloaded to validate that AbsorbanceQ is functioning properly. References: [1] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021) Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy, in press. [2] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021), Dataset for Absorbance Microscopy for Quantitative and Traceable Trypan Blue Cell Viability Measurement, National Institute of Standards and Technology, https://doi.org/10.18434/mds2-2347. [3] Zimmerman SM, Simon Jr CG, Babakhanova G (2021) AbsorbanceQ: An App for Generating Absorbance Images from Brightfield Images. J Res Natl Inst Stan 126:126039, https://doi.org/10.6028/jres.126.039.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    Dates

    Metadata Created Date June 28, 2021
    Metadata Updated Date July 29, 2022
    Data Update Frequency irregular

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date June 28, 2021
    Metadata Updated Date July 29, 2022
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-2423
    Data First Published 2021-06-23
    Language en
    Data Last Modified 2021-06-10 00:00:00
    Category Manufacturing:Process measurement and control, Manufacturing:Biomanufacturing, Manufacturing, Health:Cell therapies, Bioscience:Cell biology, Bioscience:Bioprocessing, Bioscience:Biomaterials
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 72dee9c2-77ac-47f7-bec7-0d94aebc8b18
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-2423
    License https://www.nist.gov/open/license
    Program Code 006:045
    Source Datajson Identifier True
    Source Hash f090dc81c95f735e4d247816a9a00f69475f35a0
    Source Schema Version 1.1

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