A Multivalent mRNA-lipid nanoparticle vaccine containing eight hemagglutinin antigens elicits broad neutralizing antibody responses and partially protects against influenza A challenge in swine
Influenza A virus is an important respiratory pathogen in swine, and zoonotic transmission of swine strains to humans is a risk. Control strategies against IAV in North American swine herds rely heavily on biosecurity measures and vaccination. However, the antigenic diversity of IAV circulating in swine challenges current vaccination programs, and there is a need for broadly protective vaccines or platforms that can rapidly update components to reflect the circulating diversity. mRNA-LNP vaccines have emerged as promising alternatives, offering simultaneous delivery of multiple antigens, rapid development, scalable manufacturing, and potent immunogenicity. In this study, we assessed the immunogenicity and protective capacity of monovalent and multivalent mRNA-LNP vaccines encoding eight representative IAV HA clade antigens. To our knowledge, this is the first study to objectively select multiple representative endemic swine IAV strains by quantifying genetic diversity within the phylogeny and to apply this selection to rationally design and evaluate a multivalent HA mRNA-based influenza vaccine in the swine model.
Resources
5 resources available
-
mRNA AgDataCommons.xlsx
XLSX -
Ciacci Zanella Fig 5.csv
CSV -
Ciacci Zanella Clinical Scores and Temps.csv
CSV -
Ciacci Zanella Fig 4.csv
CSV -
Ciacci Zanella Fig 2 and 3.csv
CSV
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "005:18" ] |
| contactPoint |
{ "fn": "Baker, Amy", "hasEmail": "mailto:Amy.L.Baker@usda.gov" } |
| description | <p dir="ltr">Influenza A virus is an important respiratory pathogen in swine, and zoonotic transmission of swine strains to humans is a risk. Control strategies against IAV in North American swine herds rely heavily on biosecurity measures and vaccination. However, the antigenic diversity of IAV circulating in swine challenges current vaccination programs, and there is a need for broadly protective vaccines or platforms that can rapidly update components to reflect the circulating diversity. mRNA-LNP vaccines have emerged as promising alternatives, offering simultaneous delivery of multiple antigens, rapid development, scalable manufacturing, and potent immunogenicity. In this study, we assessed the immunogenicity and protective capacity of monovalent and multivalent mRNA-LNP vaccines encoding eight representative IAV HA clade antigens. To our knowledge, this is the first study to objectively select multiple representative endemic swine IAV strains by quantifying genetic diversity within the phylogeny and to apply this selection to rationally design and evaluate a multivalent HA mRNA-based influenza vaccine in the swine model. </p> |
| distribution |
[ { "@type": "dcat:Distribution", "title": "mRNA AgDataCommons.xlsx", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "downloadURL": "https://ndownloader.figshare.com/files/63801702" }, { "@type": "dcat:Distribution", "title": "Ciacci Zanella Fig 5.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65160438" }, { "@type": "dcat:Distribution", "title": "Ciacci Zanella Clinical Scores and Temps.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65160441" }, { "@type": "dcat:Distribution", "title": "Ciacci Zanella Fig 4.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65160444" }, { "@type": "dcat:Distribution", "title": "Ciacci Zanella Fig 2 and 3.csv", "format": "csv", "mediaType": "text/csv", "downloadURL": "https://ndownloader.figshare.com/files/65160447" } ] |
| identifier | 10.15482/USDA.ADC/32025618.v1 |
| keyword |
[ "Influenza A", "Multivalent", "PARNAS", "Swine", "Vaccine", "mRNA-LNP" ] |
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2026-06-08 |
| programCode |
[ "005:040" ] |
| publisher |
{ "name": "Agricultural Research Service", "@type": "org:Organization" } |
| temporal | 2022-01-01/2026-01-01 |
| title | A Multivalent mRNA-lipid nanoparticle vaccine containing eight hemagglutinin antigens elicits broad neutralizing antibody responses and partially protects against influenza A challenge in swine |
