{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "GeneLab Outreach", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"}, "description": "The general objective of the study was to determine modulation of gene expression by environmental factors with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment which we like to call relative microgravity referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf one batch was left at 3g (3g) one batch was returned to 1g (3g>1g) while a third batch was returned to 1g but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times each time with 4 batches of 60 larvae.", "distribution": [{"@type": "dcat:Distribution", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-60", "format": "HTML", "mediaType": "text/html", "title": "Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization"}], "identifier": "nasa_genelab_GLDS-60_kdpk-5n4g", "issued": "2021-05-21", "keyword": ["array-scanning-and-feature-extraction-protocol", "growth-protocol", "hypergravity", "normalization-data-transformation-protocol", "nucleic-acid-extraction-protocol", "nucleic-acid-hybridization-to-array-protocol", "nucleic-acid-labeling-protocol", "p-mtab-43163", "p-mtab-43164", "p-mtab-43165", "p-mtab-43166", "p-mtab-43167", "p-mtab-43168", "p-mtab-43169", "treatment-protocol"], "landingPage": "https://data.nasa.gov/dataset/exposure-of-zebrafish-larvae-to-relative-microgravity-between-5-6-days-post-fertilization", "license": "http://www.usa.gov/publicdomain/label/1.0/", "modified": "2025-04-23", "programCode": ["026:005"], "publisher": {"@type": "org:Organization", "name": "National Aeronautics and Space Administration"}, "theme": ["Earth Science"], "title": "Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization"}