{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Perkin, Lindsey, C.", "hasEmail": "mailto:lindsey.perkin@usda.gov"}, "description": "<p dir=\"ltr\">The pecan weevil, <i>Curculio caryae </i>(Horn), is an obligate feeder of pecan and native hickory throughout North America. Subsequently it is a significant agricultural pest in commercial pecan orchards. In this study, we present a reference quality genome using deep-coverage, ~50x PacBio HiFi genome sequence reads, and chromatic confirmation, Hi-C, scaffolding. The final genome assembly is approximately 2.1 Gb, which was confirmed with flow cytometry. The primary genome scaffolds have an N50 of 132 Mb and a BUSCO completeness of 99.1% [S:97.5%, D:1.6%]. Furthermore, we employed PacBio long-read RNA, Iso-seq, for <i>de novo </i>annotation, followed by InterProscan to identify approximately 16,000 protein coding genes. Repeat content is extensive, contributing to >75% of the total genome. This data set provides a valuable resource for use in comparative genomics and evolutionary studies for an economically impactful group of insect pests that currently lack genomic resources.</p>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA813156", "mediaType": "text/html", "title": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA813156"}], "identifier": "10779/USDA.ADC.29329910.v1", "keyword": ["Ag100Pest", "Curculio caryae", "HiFi genome assembly", "pecan weevil"], "license": "https://creativecommons.org/publicdomain/zero/1.0/", "modified": "2026-03-23", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "temporal": "2020-09-05/2023-12-31", "title": "Genome sequencing of Curculio caryae adult female principal pseudohaplotype"}