{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Jay Reichman", "hasEmail": "mailto:reichman.jay@epa.gov"}, "description": "Fecal samples from migratory birds were collected from contaminated wetlands in the Bunker Hill Lower Basin Coeur \u2019d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected.\n\nCO1 primers with Illumina adaptors:\nCO1-F\tTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAACATTCAGCCATCTTACCCGTG\nCO1-R\tGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACAGGGAGTGATAGRAGKAGTAGG\n\nAlso, chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds, which can be found at DOI: 10.23719/1532393", "distribution": [{"accessURL": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348", "title": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1532394/Bird_CO1_SRA_metadata_Final.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Bird_CO1_SRA_metadata_Final.xlsx"}], "identifier": "https://doi.org/10.23719/1532394", "keyword": ["Amplicon-sequencing", "Birds", "CO1", "Pb toxicity"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2025-06-26", "programCode": ["020:000"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": null, "rights": null, "title": "Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: CO1 dataset"}