gov.noaa.nodc:0309778 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-07-23T08:19:31Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778) 2025-12-02 publication 2025-11-24 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0309778 NCEI Archive Management System 0309778 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Mara L. Spencer NOAA Alaska Fisheries Science Center (NMFS/AFSC) Mara.Spencer@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider Emily Slesinger NOAA Alaska Fisheries Science Center (NMFS/AFSC) emily.slesinger@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Erik V. Thuesen The Evergreen State College https://www.evergreen.edu/ HTTPS The Evergreen State College website Institution web page information principalInvestigator Thomas Hurst NOAA Alaska Fisheries Science Center (NMFS/AFSC) Thomas.Hurst@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Slesinger, Emily NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Thuesen, Erik V. The Evergreen State College https://www.evergreen.edu/ HTTPS The Evergreen State College website Institution web page information author Hurst, Thomas P. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information contributor The Evergreen State College https://www.evergreen.edu/ HTTPS The Evergreen State College website Institution web page information contributor tableDigital This dataset contains laboratory experiment data that were collected to examine potential effects of warming and ocean acidification in high latitude marine ecosystems. Warming in high latitude marine ecosystems is leading to the borealization of Arctic communities. Species-specific responses to temperature provide insight into potential co-occurrence or competitive advantage between Arctic and boreal species. Ocean acidification may also lead to unique species-specific responses. At the Pacific-Arctic interface, larval distributions of the boreal Pacific cod (Gadus macrocephalus) are increasingly overlapping with those of Arctic cod (Boreogadus saida). We assessed larval metabolic capacities by measuring metabolic enzyme activities of citrate synthase (CS; aerobic metabolism), lactate dehydrogenase (LDH; anaerobic metabolism), and β-hydroxyacyl CoA dehydrogenase (HOAD; fatty acid metabolism). Throughout early development, Pacific cod enzyme activities, including glycolytic capacity, were higher, and fatty acid metabolism lower than Arctic cod enzyme activities. These responses may reflect a more active larval lifestyle of Pacific cod. Separately, larvae were reared in multiple temperatures (Pacific cod: 3, 6, 10°C; Arctic cod 1.8, 5, 7.3°C) and pCO2 levels (ambient = ~350 μatm; high = ~1500 μatm). At the cold temperature, Pacific cod enzyme activities were higher than at the control temperature, indicating they were acclimating but less cold adapted than Arctic cod. Arctic cod HOAD activity and LDH:CS ratio were elevated under warmer temperatures suggesting increased energy demand. Elevated pCO2 levels only affected larvae at their control temperature and resulted in decreased Pacific cod HOAD activity and increased Arctic cod CS and HOAD activities. This indicates differing sensitivities to ocean acidification between the species. Overall, Pacific cod may continue to be constrained in their northern habitat by cold temperatures, but under slight warming to optimal growing temperatures, Pacific cod will have competitive advantage over Arctic cod. This dataset is available to the public for a wide variety of uses including scientific research and analysis. Funding Information: NOAA’s Ocean Acidification Program (#20903 to Thomas P. Hurst) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0309778 Preview graphic PNG 0309778 NCEI ACCESSION NUMBER 2025-11-21 publication DISSOLVED INORGANIC CARBON (DIC) partial pressure of carbon dioxide - water pH SPECIES IDENTIFICATION SPECIES IDENTIFICATION - DRY WEIGHT total alkalinity WATER TEMPERATURE theme NODC DATA TYPES THESAURUS carbon dioxide (CO2) gas analyzer pH sensor temperature sensor titrator instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory experiments physical survey - biological theme NODC OBSERVATION TYPES THESAURUS NOAA Alaska Fisheries Science Center The Evergreen State College dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Alaska Fisheries Science Center dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS Arctic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type CO2_trmt CS_P CS_WW DIC DPH DW DW_CONC Date HOAD_CS HOAD_P HOAD_WW LDH_CS LDH_P LDH_WW LEN PROTEIN_CONC SPP TA TEMP_trmt pCO2 pH theme Provider Variable Abbreviations CO2 ANALYZERS > CO2 ANALYZERS PH METERS > PH METERS TEMPERATURE SENSORS > TEMPERATURE SENSORS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian OCEAN > ARCTIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NOAA AFSC Laboratory Newport Oregon place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Slesinger, Emily; Thuesen, Erik V.; Hurst, Thomas P. (2025). Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0309778. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0). SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Slesinger, Emily; Tyler, Erin F.; Mundorff, Samantha; Youn, Kathryn R.; Edmondson, Leo; Hurst, Thomas P. (2026). Data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification from 2022-07-01 to 2024-08-31 (NCEI Accession 0313059). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/cvec-sa43. 2026-03-06 publication International DOI Foundation (IDF) https://doi.org/10.25921/cvec-sa43 NOAA National Centers for Environmental Information gov.noaa.nodc:0313059 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0313059.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0313059.html Related dataset information Slesinger, Emily NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Tyler, Erin F. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Mundorff, Samantha Northeastern University http://www.northeastern.edu HTTP Northeastern University website Institution web page information author Youn, Kathryn R. Hendrix College author Edmondson, Leo University of New England author Hurst, Thomas P. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project eng; USA utf8 environment oceans biota -124.043955 -124.043955 44.621316 44.621316 2022-04-13 2023-06-28 This data package (Submission ID: BJKHBMF0Y) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Originator data format 0.108 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/0309778 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/309778 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/309778 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0237/0309778/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0309778 v1.1 was published. 2025-12-02T18:11:33Z NCEI Accession 0309778 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0309778/1.1 HTTPS NCEI Accession 0309778 v1.1 published 2025-12-02T18:11:33Z download nonGeographicDataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer); Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity); CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pH; Abbreviation: pH; pH scale: SEAWATERSCALE(SWS); In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 5.9±0.4°C; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: µatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Species identification; Abbreviation: SPP; Unit: none; Detailed sampling and analyzing information: Coded as "Acod" for Arctic cod, "Pcod" for Pacific cod.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus Parameter or Variable: CO2 treatment; Abbreviation: CO2_trmt; Unit: none; Detailed sampling and analyzing information: Coded as "amb" for ambient level or "high" for high treatment level. A Durafet III pH probe (Honeywell) in each of the high pCO2 treatment header tanks regulated the injection of CO2 based on achieving temperature-dependent pH targets (see Slesinger et al., 2024). Parameter or Variable: Water temperature treatment designation; Abbreviation: TEMP_trmt; Unit: °C; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded as “1.8”, “5”, or “7.3” for the targeted treatment temperatures during the experiment. Parameter or Variable: lactate dehydrogenase to citrate synthase ratio; Abbreviation: LDH_CS; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: CALCULATED; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: β-hydroxyacyl CoA dehydrogenase to citrate synthase ratio; Abbreviation: HOAD_CS; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: CALCULATED; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: citrate synthase protein; Abbreviation: CS_P; Unit: mg protein; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: β-hydroxyacyl CoA dehydrogenase protein; Abbreviation: HOAD_P; Unit: mg protein; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Singer TD, Ballantyne JS (1989) Absence of extraheptic lipid oxidation in a freshwater elasmobranch, the dwarf stingray Potamotrygon magdalenae: evidence from enzyme activities. J Exp Zool 251:355-360.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: lactate dehydrogenase protein; Abbreviation: LDH_P; Unit: mg protein; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: citrate synthase wet-weight; Abbreviation: CS_WW; Unit: mg wet-weight; Observation type: Laboratory experiment; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: β-hydroxyacyl CoA dehydrogenase wet-weight; Abbreviation: HOAD_WW; Unit: mg wet-weight; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Singer TD, Ballantyne JS (1989) Absence of extraheptic lipid oxidation in a freshwater elasmobranch, the dwarf stingray Potamotrygon magdalenae: evidence from enzyme activities. J Exp Zool 251:355-360.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: lactate dehydrogenase wet-weight; Abbreviation: LDH_WW; Unit: mg wet-weight; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher); Detailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD ��mol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.; Method reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Larval age in days post hatch; Abbreviation: DPH; Unit: days; Observation type: Laboratory experiment Parameter or Variable: Protein concentration; Abbreviation: PROTEIN_CONC; Unit: mg per mL; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Spectrophotometer (SpectraMax Plus 384, Molecular Devices); Detailed sampling and analyzing information: Protein concentrations (mg mL-1) were determined spectrophotometrically (SpectraMax Plus 384, Molecular Devices) at 37°C using a BCA kit (ThermoFisher).; Method reference: https://documents.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011430_Pierce_BCA_Protein_Asy_UG.pdf; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Sampling date; Abbreviation: Date; Unit: none Parameter or Variable: Mean dry weight; Abbreviation: DW; Unit: mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Proportion of dry weight to wet weight; Abbreviation: DW_CONC; Unit: none; Observation type: l; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: CALCULATED; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard length; Abbreviation: LEN; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (LEN; mm).; Biological subject: Arctic cod, Pacific cod; Species ID: Boreogadus saida, Gadus macrocephalus; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian carbon dioxide (CO2) gas analyzer carbon dioxide (CO2) gas analyzer measures gaseous carbon dioxide Carbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption. Carbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water. To measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water. pH sensor pH sensor generic meter used to measure pH Any of a class of instruments used to quantify pH in a water or other type of sample. temperature sensor temperature sensor A sensor used to measure temperature. titrator titrator titrator