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        <gco:CharacterString>This data package includes a quantitative analysis of non-coral communities at sites along a water quality gradient off of Aua, American Samoa in 2022. These datasets were funded by the NOAA Coral Reef Conservation Program (CRCP) Project Number 31303 to study effects of land-based sources of pollution (LBSP) in Aua, American Samoa. In September 2022 Ecosystem Sciences Division (ESD) scientists of the Pacific Islands Fisheries Science Center (PIFSC) flew into American Samoa to survey 18 sites along a water quality gradient off of Aua. Three datasets are provided of the taxa species and counts of benthic foraminifera, benthic microalgae, and macroinvertebrates from samples collected between 9-28 September 2022. Samples were preserved in the field, and brought back to the NOAA Inouye Regional Center (IRC) and analyzed via microscopy. To analyze benthic foraminifera abundance, sediment samples were collected using a small sediment corer (60 ml syringe with the tip removed and a stopper placed there instead). Only the top 3 cm were retained. Under the microscope, benthic foraminifera were picked out of the sediment and identified. To analyze benthic microalgae, microscope slides were deployed on the seafloor at each site for 2 to 3 weeks. The benthic microalgae that settled was fixed and preserved in Lugol's solution and analyzed. Microalgae included diatoms (pennate and centric), dinoflagellates, chlorophyta, and cyanobacteria. To analyze macroinvertebrates: plastic scouring pads were deployed and attached to the substratum with zip-ties for 2 to 3 weeks. The scouring pads were removed after the settlement period, all plastic zip-ties and additional waste were removed from the reef. Macroinvertebrates that settled on the scouring pad were placed into sampling jars and fixed and preserved with 4% formalin, and subsequently analyzed under the microscope.

These quantitative non-coral community surveys were one of several surveys conducted at the same sites across Aua reef in September 2022. Other surveys described and archived separately include surveys of water quality, CTD casts, coral demography, benthic imagery/benthic cover, and coral demography. These can be accessed under the 'Related Items' section of the InPort metadata record.</gco:CharacterString>
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NOAA Coral Reef Conservation Program (CRCP) funds research under the Land-Based Sources of Pollution. This mission (Project number 31303) sought out to examine the impacts of LBSP on reef communities (coral and non-coral) in Aua, a high priority watershed in American Samoa. This mission builds on prior assessment and monitoring surveys of benthic reef communities conducted in Faga'alu and Vatia bays in 2012, 2015, and 2022 to address National and Jurisdictional goals to reduce the impacts of LBSP in local priority watersheds. Together with periodic monitoring, this coral reef community baseline for Aua reef will allow scientists to quantify temporal changes in the condition and integrity and enable Jurisdictional and Federal resource managers to make informed decisions pertaining to the protection and preservation of critical habitats. The overall effort required to evaluate the effectiveness of management strategies is significant and requires close coordination between local and Federal efforts.</gco:CharacterString>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="platform">platform</gmd:MD_KeywordTypeCode>
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          <gmd:keyword>
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          <gmd:keyword>
            <gco:CharacterString>EARTH SCIENCE &gt; Oceans &gt; Marine Biology &gt; Marine Invertebrates &gt; Macroinvertebrates</gco:CharacterString>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="theme">theme</gmd:MD_KeywordTypeCode>
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        </gmd:MD_Keywords>
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      <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
          <gmd:keyword>
            <gmx:Anchor xlink:href="https://cmr.earthdata.nasa.gov/kms/concept/fbec5145-79e6-4ed0-a804-6228aa6daba5" xlink:actuate="onRequest">EARTH SCIENCE &gt; BIOLOGICAL CLASSIFICATION</gmx:Anchor>
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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://cmr.earthdata.nasa.gov/kms/concept/abc6f016-d1f0-4725-b847-639de054d13f" xlink:actuate="onRequest">EARTH SCIENCE &gt; BIOLOGICAL CLASSIFICATION &gt; ANIMALS/INVERTEBRATES</gmx:Anchor>
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          <gmd:keyword>
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          <gmd:keyword>
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          <gmd:keyword>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="place">place</gmd:MD_KeywordTypeCode>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="place">place</gmd:MD_KeywordTypeCode>
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Observation Category: laboratory analysis;
Sampling Instrument: microscope;
Sampling and Analyzing Method: Plastic scouring pads were deployed and attached to the substratum via zip-ties for 2 to weeks. The scouring pads were removed after the settlement period, all plastic zip-ties and additional waste were removed from the reef. Macroinvertebrates that settled on the scouring pad were placed into sampling jars and fixed and preserved with 4% formalin, and subsequently identified to major taxonomic categories and counted under the microscope. Macroinvertebrate abundance of major taxonomic groups include copepods (oncaeidae, harpacticoida, cyclopoida, sapphirinidae, unknown), isopods, ostracods, amphipods, tanaids, cumaceans, pteropods, gastropods, halacaridae, chaetognaths, vermitid tube snails, nematods, polychaetes, terebellidae, capitellidae, crustacean nauplii, crustacean larvae, shrimp larvae, mantis shrimp larvae, hermit crab, crab zoea, crab megalopa, crab juvenile, fish larvae, brittle star larvae, urchin larvae, mollusk veligers, bivalve larvae, and barnacle larvae;
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Sampling and Analyzing Method: Abundance of benthic microalgae was also measured.  Microscope slides were deployed on the seafloor at each site for 2 to 3 weeks.  The  benthic microalgae that settled on the slides were fixed and preserved in Lugol's solution and analyzed. Microalgae included diatoms (pennate and centric), dinoflagelates, chlorophyta, and cyanobacteria, and were identified and counted under the microscope.;
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Units: count;
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Sampling and Analyzing Method: Counts of cyanobacteria were included in the benthic microalgal abundance measurements. Microscope slides were deployed on the seafloor at each site for 2 to 3 weeks and the benthic microalgae that settled was fixed and preserved in Lugol's solution and analyzed. Microalgae included diatoms (pennate and centric), dinoflagellates, chlorophyta, and cyanobacteria, and were identified and counted under the microscope.</gco:CharacterString>
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