gov.noaa.nodc:0301881 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-03-26T08:43:06Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Total pH and alkalinity and other water quality, carbonate chemistry, and physiological variables collected from laboratory experiment observations using the m-cresol dye spectrophotometric and open-cell potentiometric methods and other methods in the Mid-Atlantic region from 2019-07-26 to 2019-09-12 (NCEI Accession 0301881) 2025-02-21 publication 2025-02-18 creation International DOI Foundation (IDF) 10.25921/2zv5-yw49 NOAA National Centers for Environmental Information gov.noaa.nodc:0301881 NCEI Archive Management System 0301881 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Annie Schatz Virginia Institute of Marine Science (VIMS) kschatz@umd.edu) resourceProvider Virginia Institute of Marine Science (VIMS) resourceProvider Annie Schatz Virginia Institute of Marine Science (VIMS) kschatz@umd.edu) principalInvestigator Jan McDowell Virginia Institute of Marine Science (VIMS) principalInvestigator Emily Rivest Virginia Institute of Marine Science (VIMS) principalInvestigator Schatz, Annie Virginia Institute of Marine Science (VIMS) author McDowell, Jan R. Virginia Institute of Marine Science (VIMS) author Rivest, Emily B. Virginia Institute of Marine Science (VIMS) author Virginia Institute of Marine Science (VIMS) contributor tableDigital This dataset contains discrete water sample and physiological data from a laboratory experiment that took place July 26 - September 12, 2019 to observe how ocean acidification might impact the development of juvenile eastern oysters in the Chesapeake Bay within the Mid-Atlantic region. Oysters were raised in three pH treatments, with three replicate tanks each, representing average (7.8), low (7.4), and extreme low (7.0) pH conditions within the mesohaline region of the Chesapeake Bay. Carbonate chemistry (pH (total) and total alkalinity) and water quality (temperature, salinity, dissolved oxygen) conditions of each tank were monitored three times per week, while physiological conditions were measured at the start of the experiment when oysters were 15 days post fertilization (dpf) and then at 18, 26, 35, and 70 dpf. This data was collected to test whether ocean acidification experienced during oyster settlement (a proxy for metamorphosis) would impact them during settlement and a month after settlement during their juvenile stage (referred to as carryover effects) Funding Information: National Sea Grant College Program (NA17OAR4170235) Funding Information: Virginia Department of Agriculture and Consumer Services (712) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0301881 Preview graphic PNG 0301881 NCEI ACCESSION NUMBER 2025-02-15 publication ARAGONITE SATURATION STATE CALCITE CONCENTRATION DISSOLVED INORGANIC CARBON (DIC) pH respiration rates SALINITY shell parameters (length or weight) total alkalinity WATER TEMPERATURE theme NODC DATA TYPES THESAURUS carbon dioxide (CO2) gas analyzer temperature sensor thermosalinograph titrator instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory experiments physical survey - biological theme NODC OBSERVATION TYPES THESAURUS Virginia Institute of Marine Science dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS Virginia Institute of Marine Science dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) project NODC PROJECT NAMES THESAURUS Chesapeake Bay North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication CWM/VIMS > Virginia Institute of Marine Science, College of William and Mary dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > RESPIRATION RATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBONATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type Aragonite Saturation State Calcite Saturation State Dissolved Inorganic Carbon Lipid Peroxidation Respiration Rate Salinity Shell Size (Length) Survival Temperature Total Alkalinity Total Antioxidant Capacity Total Protein Triglyceride Content pH (total) theme Provider Variable Abbreviations CO2 ANALYZERS > CO2 ANALYZERS TEMPERATURE SENSORS > TEMPERATURE SENSORS THERMOSALINOGRAPHS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Chesapeake Bay place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Schatz, Annie; McDowell, Jan R.; Rivest, Emily B. (2025). Total pH and alkalinity and other water quality, carbonate chemistry, and physiological variables collected from laboratory experiment observations using the m-cresol dye spectrophotometric and open-cell potentiometric methods and other methods in the Mid-Atlantic region from 2019-07-26 to 2019-09-12 (NCEI Accession 0301881). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/2zv5-yw49. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license This dataset has been dedicated to the public domain under a Creative Commons CC0 1.0 Universal (CC0 1.0) Public Domain Dedication. SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Global Coastal Program Data: North America East Coast 2022 publication https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/north_america_east.html HTTPS https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/north_america_east.html Related resource information NOAA National Centers for Environmental Information (NCEI) publisher documentDigital crossReference project eng; USA utf8 environment oceans -76.457012 -76.455531 37.389506 37.390368 2019-07-26 2019-09-12 This data package (Submission ID: BH3LD6NYR) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. 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Originator data format 0.272 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0301881.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://doi.org/10.25921/2zv5-yw49 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/301881 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/301881 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0235/0301881/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0301881 v1.1 was published. 2025-02-21T22:13:15Z NCEI Accession 0301881 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0301881/1.1 HTTPS NCEI Accession 0301881 v1.1 published 2025-02-21T22:13:15Z download nonGeographicDataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: Dissolved Inorganic Carbon; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: See Below; Sampling instrument: N/A; Analyzing instrument: N/A; Detailed sampling and analyzing information: Total alkalinity and pHtotal were used to calculate the suite of carbonate chemistry parameters using the Seacarb package in R (Gattuso et al., 2021), complementary temperature and salinity data, and the following constants: K1 and K2 for carbonic acid (Lueker et al. 2000), KF (Perez and Fraga 1987), total boron (Lee et al. 2010), and KSO4 (Dickson et al. 1990).; Uncertainty: ~324 umol/kg; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Total alkalinity; Abbreviation: Total Alkalinity; Unit: umol per kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Calculation method and parameters: See Below; Sampling instrument: PYREX© Narrow Mouth Reagent Storage Bottles With Standard Taper Stopper; Analyzing instrument: Metrohm 855 Robotic Titrosampler; Detailed sampling and analyzing information: Total alkalinity was measured using open-cell potentiometric titrations following best practices (SOP 3b; Dickson et al., 2007). Total alkalinity was measured following SOP 3b (Dickson, 2007) and using a Metrohm 855 Robotic Titrosampler with ~0.1M HCl in 0.6M NaCl (A. Dickson Laboratory, Scripps Institution of Oceanography). The accuracy of the Titrosampler was ± 10 µmol kg SW-1, assessed using a certified reference material (A. Dickson Laboratory, Scripps Institution of Oceanography), and water samples were run in technical duplicates.; Standardization description: The electrode on the titraitor was calibrated using pH standards and then measured against a certified reference material; Standardization frequency: Every time the titrator was run; CRM manufacturer: A. Dickson Laboratory, Scripps Institution of Oceanography; Preservation method: Frozen at -20 degrees C; Uncertainty: 179 umol/kg; Method reference: SOP 3b; Dickson et al., 2007; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: pH; Abbreviation: pH (total); pH scale: TOTALSCALE(T); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Calculation method and parameters: See Below; Sampling instrument: Scintillation Vial; Analyzing instrument: Thermo Scientific™ Evolution™ 201 UV-Visible spectrophotometer; Detailed sampling and analyzing information: Samples were collected directly from experimental tanks via a syphon into a tilted scintillation vial to reduce turbulence and air bubbles that could cause atmospheric CO2 to dissolve into the sample. Water pH (total scale) was measured in technical triplicates following SOP 6b (Dickson, 2007) and using a temperature-controlled Thermo Scientific™ Evolution™ 201 UV-Visible spectrophotometer. Absorbance was measured with and without m-cresol purple dye at 730, 578, and 434 nm at 25 degrees C.; Standardization description: When you prepare the m-cresol dye, you calibrate the dye to a series of manipulated pH conditions using 1-micron filtered sea water with various additions of NaOH and HCl.; Standardization frequency: At the start of the experiment using the filtered sea water with the experimental salinity value; Temperature correction method: Seacarb; At what temperature was pH reported: in-situ; Uncertainty: 0.09 units; Method reference: Dickson et al., 2007; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Aragonite saturation state; Abbreviation: Aragonite Saturation State; Unit: N/A; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: See Below; Sampling instrument: N/A; Analyzing instrument: N/A; Duration: 6 weeks; Detailed sampling and analyzing information: Total alkalinity and pHtotal were used to calculate the suite of carbonate chemistry parameters using the Seacarb package in R (Gattuso et al., 2021), complementary temperature and salinity data, and the following constants: K1 and K2 for carbonic acid (Lueker et al. 2000), KF (Perez and Fraga 1987), total boron (Lee et al. 2010), and KSO4 (Dickson et al. 1990).; Uncertainty: 0.14; Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Calcite saturation state; Abbreviation: Calcite Saturation State; Unit: N/A; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: See Below; Sampling instrument: N/A; Analyzing instrument: N/A; Duration: 6 weeks; Detailed sampling and analyzing information: Total alkalinity and pHtotal were used to calculate the suite of carbonate chemistry parameters using the Seacarb package in R (Gattuso et al., 2021), complementary temperature and salinity data, and the following constants: K1 and K2 for carbonic acid (Lueker et al. 2000), KF (Perez and Fraga 1987), total boron (Lee et al. 2010), and KSO4 (Dickson et al. 1990).; Uncertainty: 0.23; Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: Schatz Parameter or Variable: Salinity; Abbreviation: Salinity; Unit: N/A; Observation type: Laboratory experiment; Sampling instrument: YSI Pro Plus; Analyzing instrument: YSI Pro Plus; Duration: 6 weeks; Detailed sampling and analyzing information: Three times per week, prior to each water change, water samples were collected from each aquarium and from the 1 µm-filtered water used to re-fill the aquaria, accompanied by measurements of temperature, dissolved oxygen, pH, conductivity, and salinity using a YSI Pro Plus (YSI Inc., Yellow Springs, OH).; Uncertainty: 0.18; Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie; Researcher institution: Schatz Parameter or Variable: Water temperature; Abbreviation: Temperature; Unit: degrees celsius; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Sampling instrument: YSI Pro Plus and Signet, LLC 2350 thermal probe; Analyzing instrument: YSI Pro Plus and Signet, LLC 2350 thermal probe; Duration: 6 weeks; Detailed sampling and analyzing information: Three times per week, prior to each water change, water samples were collected from each aquarium and from the 1 µm-filtered water used to re-fill the aquaria, accompanied by measurements of temperature, dissolved oxygen, pH, conductivity, and salinity using a YSI Pro Plus (YSI Inc., Yellow Springs, OH). Additionally, surrounding each aquarium was a recirculating water jacket containing a Signet, LLC 2350 thermal probe. Heat exchangers connected to external closed loops of recirculating hot and cold water were used to control the temperature of the water jacket. Water temperature and pH in each aquarium were controlled and maintained at setpoint conditions using a software control system that received data every minute from the pH electrodes and thermal probes in each aquarium. When aquarium temperature deviated from its setpoint, a four-way valve was autonomously opened to send hot or cold water through the heat exchanger in the aquarium’s water jacket, as needed.; Uncertainty: 0.37; Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie; Researcher institution: Schatz Parameter or Variable: Ferric reduction/antioxidant potential; Abbreviation: Total Antioxidant Capacity; Unit: umol/g protein; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: ferric reducing/antioxidant potential assay; Sampling instrument: Pipette; Analyzing instrument: Molecular Devices SpectraMax® iD3 microplate reader; Duration: 6 weeks; Detailed sampling and analyzing information: Oysters were volumetrically sampled and stored at -80 degrees Celsius before analysis with the ferric reducing/antioxidant potential assay; Method reference: (Schatz et al., 2024) (Griffin and Bhagooli, 2004); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Total Protein Content; Abbreviation: Total Protein; Unit: ng/individual; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: Bicinchoninic Acid Protein Assay; Sampling instrument: Pipette; Analyzing instrument: Molecular Devices SpectraMax® iD3 microplate reader; Duration: 6 weeks; Detailed sampling and analyzing information: Oysters were volumetrically sampled and stored at -80 degrees Celsius until analysis using the Thermo Scientific, 23225 assay kit; Method reference: (Schatz et al., 2024)(Smith et al., 1985); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Malondialdehyde (MDA); Abbreviation: Lipid Peroxidation; Unit: nmol malondialdehyde/ ug protein; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: Lipid Peroxidation Malondialdehyde Assay; Sampling instrument: Pipette; Analyzing instrument: Molecular Devices SpectraMax® iD3 microplate reader; Duration: 6 weeks; Detailed sampling and analyzing information: Lipid peroxidation was measured as malondialdehyde (MDA) content using the Lipid Peroxidation Malondialdehyde Assay kit (Abcam 118970). Lipid peroxidation in oyster tissue was measured following the kit instructions. Briefly, oyster samples were homogenized in the lysis buffer provided (1:100 2,6-Di-tert-butyl-4-methylphenol [BHT]). Oysters from the settlement and early juvenile phase (i.e., week one samples) were homogenized using sonication, while juvenile oyster samples from the end of the experiment (i.e., week 5 samples) were homogenized with an MP BiomedicalTM FastPrep-24TM bead beating grinder using a lysing mixture of ceramic, silica, and glass beads in 2 mL centrifuge tubes (MP BiomedicalTM Lysing Matrix E TM). Absorbance was measured at 532 nm using a microplate reader (Molecular Devices SpectraMax iD3). Samples from each treatment replicate were measured in technical triplicates. Duplicate standard curves of known MDA concentrations (0 – 5 nmol) on each plate were used to calculate MDA content in oyster samples. MDA content was then normalized to total protein content of the homogenate.; Method reference: (Abcam 118970); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Triglyceride Content; Abbreviation: Triglyceride Content; Unit: nmol/ mg protein; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: CALCULATED; Calculation method and parameters: Triglyceride Assay; Sampling instrument: Pipette; Analyzing instrument: Molecular Devices SpectraMax® iD3 microplate reader; Duration: 6 weeks; Detailed sampling and analyzing information: Triglyceride (TG) content was measured using the Triglyceride Assay Kit following the manufacturer’s instructions (Abcam 65336). Oyster samples were homogenized via sonication or bead beating, as above, in 5% NP-40 and analyzed in technical duplicate pairs. Absorbance was measured at 570 nm using the microplate reader. Duplicate standard curves of known TG concentrations (0 – 10 nmol) on each plate were used to calculate TG concentrations in oyster samples. TG content was then normalized to total protein content of the homogenate.; Method reference: (Abcam 65336); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Respiration rates; Abbreviation: Respiration Rate; Unit: pmol oxygen/ ng protein per hr; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Calculation method and parameters: Endpoint respirometry using micro biological oxygen demand vials; Sampling instrument: Airtight syringe; Analyzing instrument: PreSens Microx 4 Oxygen Microsensor; Duration: 6 weeks; Detailed sampling and analyzing information: Starved oysters were placed into micro biological oxygen demand (µBOD) vials filled with their respective, oxygen-saturated, 0.2 µm-filtered treatment water. Oysters from each treatment replicate were measured in triplicate with 10 oysters per µBOD vial. Blank vials filled with filtered treatment water but not oysters were prepared for each treatment group. µBOD vials were incubated at their respective treatment temperatures for approximately 2 hours before end-point measurements of the oxygen concentration were made using a PreSens Microx 4 Oxygen Microsensor (Regensburg, Germany). An airtight syringe was used to transfer a subsample of vial water into a temperature-controlled glass measurement cell where the microsensor was located. Three consecutive measurements of dissolved oxygen concentration were recorded after a two-minute stabilization period. The oxygen microsensor was calibrated using a 0% O2 (saturated Na2SO3) and a 100% O2 (saturated filtered seawater). Oysters from each µBOD vial were preserved at -80°C for later analysis of total protein. Oxygen consumption rates in vials with oysters were calculated by subtracting background microbial oxygen consumption (using blank BOD vial measurements) and adjusting for sensor drift, vial volume, incubation time, and biomass (estimated using total protein).; Method reference: (Marsh and Manahan, 1999); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Shell Size; Abbreviation: Shell Size (Length); Unit: micrometers; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Sampling instrument: Leica DM compound microscope with Leica MC170 HD camera; Analyzing instrument: ImageJ software or calipers; Duration: 6 weeks; Detailed sampling and analyzing information: The density was calculated from a minimum of three sequential counts of 50 µL of the concentrated, homogenized oyster sub-sample, during which images of individual oysters (n ≅ 100) were also captured using a Leica DM compound microscope (Deerfield, IL) with a Leica MC170 HD camera. ImageJ (Schneider et al., 2012) was used to measure the longest length (hinge to bill) of each oyster from images captured with a Leica S9i dissection microscope and integrated camera. Juvenile shell length after five weeks (N=50) was measured using calipers.; Method reference: (Schatz et al., 2024) (Schneider et al., 2012); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS Parameter or Variable: Percent Survival; Abbreviation: Survival; Unit: Percent; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: MEASURED; Calculation method and parameters: See below; Sampling instrument: Pipette; Analyzing instrument: Leica DM compound and Leica S9i dissection microscopes; Duration: 6 weeks; Detailed sampling and analyzing information: The density was calculated from a minimum of three sequential counts of 50 µL of the concentrated, homogenized oyster sub-sample. Density was then compared to the previous sampling period to calculate percent survival.; Method reference: (Schatz et al., 2024); Biological subject: Crassostrea virginica; Species ID: 1545929; Researcher name: Annie Schatz; Researcher institution: VIMS asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian carbon dioxide (CO2) gas analyzer carbon dioxide (CO2) gas analyzer measures gaseous carbon dioxide Carbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption. Carbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water. To measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water. temperature sensor temperature sensor A sensor used to measure temperature. thermosalinograph thermosalinograph automated sea surface temperature and salinity instrument, usually attached to the hull of a ship Thermosalinograph is an automated Sea Surface Temperature and Salinity measurement system making measurement on board a ship using a water intake. [https://www.aoml.noaa.gov/phod/tsg/about.php 2021-01-29] titrator titrator titrator