{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "bureauCode": [
    "006:55"
  ],
  "contactPoint": {
    "fn": "Jeffrey W. Hudgens",
    "hasEmail": "mailto:jeffrey.hudgens@nist.gov"
  },
  "description": "This tutorial provides  mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.",
  "distribution": [
    {
      "accessURL": "https://doi.org/10.18434/M32151",
      "title": "DOI Access for Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry"
    },
    {
      "description": "STL Files for parts used to construct the Multi-channel Temperature Controller",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z",
      "format": "7Z",
      "mediaType": "application/octet-stream",
      "title": "STL Files for parts used to construct the Multi-channel Temperature Controller"
    },
    {
      "description": "This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column.",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx",
      "format": "word processing document",
      "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
      "title": "ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"
    },
    {
      "description": "This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z",
      "mediaType": "application/octet-stream",
      "title": "Multi-Channel Valve Controller STL files"
    },
    {
      "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller.",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf",
      "mediaType": "application/pdf",
      "title": "Multi-Channel Valve Controller"
    },
    {
      "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf",
      "mediaType": "application/pdf",
      "title": "Construction information for the Multi-channel Temperature Controller"
    },
    {
      "description": "This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf",
      "mediaType": "application/pdf",
      "title": "HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"
    },
    {
      "description": "This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf.",
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z",
      "format": "7Z",
      "mediaType": "application/octet-stream",
      "title": "STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for STL Files for parts used to construct the Multi-channel Temperature Controller"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for Construction information for the Multi-channel Temperature Controller"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for Multi-Channel Valve Controller STL files"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf.sha256",
      "mediaType": "text/plain",
      "title": "SHA256 File for Multi-Channel Valve Controller"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf",
      "mediaType": "application/pdf"
    },
    {
      "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf.sha256",
      "mediaType": "text/plain"
    }
  ],
  "identifier": "ark:/88434/mds2-2151",
  "issued": "2020-04-14",
  "keyword": [
    "hydrogen-deuterium exchange",
    "liquid chromatography",
    "mass spectrometry",
    "peptide",
    "precision",
    "protein",
    "proteolysis",
    "proteomics",
    "reference material",
    "temperature control."
  ],
  "landingPage": "https://data.nist.gov/od/id/mds2-2151",
  "language": [
    "en"
  ],
  "license": "https://www.nist.gov/open/license",
  "modified": "2019-02-20 00:00:00",
  "programCode": [
    "006:045"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Institute of Standards and Technology"
  },
  "theme": [
    "Bioscience:Bioprocessing",
    "Bioscience:Proteomics",
    "Chemistry:Analytical chemistry",
    "Metrology:Amount of substance",
    "Physics:Biological physics"
  ],
  "title": "Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry"
}