{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Katy E Klymus", "hasEmail": "mailto:kklymus@usgs.gov"}, "description": "Data describe a mesocosm study for the detection of Mucket (Ortmanniana ligamentina) female and male mitotype environmental DNA (eDNA) during a spawning event. Water samples (approximately 1L) were collected every Monday and Thursday for 4 weeks during August 2021 from a pond located at Columbia Environmental Research Center containing O. ligamentina and analyzed for the presence of sex-specific eDNA. Parameters described in this data release include qPCR results and associated quality assurance measurements from the mesocosm water samples.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P14PSVQ9", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.689a1653d4be02504d348cf3.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_689a1653d4be02504d348cf3", "keyword": ["Columbia Environmental Research Center", "Missouri", "USGS:689a1653d4be02504d348cf3", "aquatic biology", "biota", "environmental DNA", "freshwater ecosystems", "mollusks"], "modified": "2025-08-11T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-92.284312, 38.910784, -92.274450, 38.912612", "theme": ["geospatial"], "title": "Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event"}