{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "GeneLab Outreach", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"}, "description": "Genome-wide detection of novel non-coding RNAs in S. cerevisiae by modulating an RNase P pathway through the depletion of a component RPP1. Nearly 400,000 36-mer oligonucleotide probes tiling the entire yeast genome including the mitochondrial chromosome with an average gap of 10 bases between two consecutive probes were synthesized on glass slides using a mask-less array synthesizer. RNA samples for hybridizing to the arrays were extracted from a conditional lethal allele of S. cerevisiae created by placing the RPP1 gene under control of GAL10 promoter. It allowed the expression of RPP1 in galactose-containing culture medium but suppressed its expression in glucose-containing medium. A wild-type isogenic strain was used as a control. Both strains were initially grown in galactose-containing medium and subsequently transferred and resuspended into glucose-containing medium. Eight arrays were hybridized with RNA extracted from the Rpp1-depleted cells at 0 4 7 12 16 21 30h and the control cell at 30h after initial transfer to glucose-containing medium.", "distribution": [{"@type": "dcat:Distribution", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-10", "format": "HTML", "mediaType": "text/html", "title": "Global Identification of Noncoding RNAs in S. cerevisiae"}], "identifier": "nasa_genelab_GLDS-10", "issued": "2018-06-26", "keyword": ["bioassay_data_transformation", "data-collection", "data-transformation", "feature_extraction", "labeling", "normalization-data-transformation", "nucleic-acid-hybridization", "p-gse4275-1", "p-gse4275-2", "p-gse4275-3", "rna-extraction", "timepoint"], "landingPage": "https://data.nasa.gov/dataset/global-identification-of-noncoding-rnas-in-s-cerevisiae", "modified": "2025-04-23", "programCode": ["026:005"], "publisher": {"@type": "org:Organization", "name": "National Aeronautics and Space Administration"}, "theme": ["Earth Science"], "title": "Global Identification of Noncoding RNAs in S. cerevisiae"}