{"@type": "dcat:Dataset", "DOI": "10.15121/1372392", "accessLevel": "public", "bureauCode": ["019:20"], "contactPoint": {"@type": "vcard:Contact", "fn": "Caroline Ajo-Franklin", "hasEmail": "mailto:cajo-franklin@lbl.gov"}, "dataQuality": true, "description": "This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.\n\nA key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),\n\nReferences:\nKinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.\nHoworka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.\n", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://gdr.openei.org/files/951/Gd-binding-S-layers.xlsx", "description": "This data set characterizes binding of Gd3+ to engineered nanosheets at 40C. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Gd3+-binding domains on these sheets. Their ability to bind Gd3+ is compared to S-layer nanosheets that do not contain Gd3+-binding domains.", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Gd-Binding S-Layers.xlsx"}, {"@type": "dcat:Distribution", "accessURL": "https://gdr.openei.org/files/951/Zn-binding-S-layers.xlsx", "description": "This data set characterizes binding of Zn2+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains.", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Zn-Binding S-Layers.xlsx"}], "identifier": "https://data.openei.org/submissions/7119", "issued": "2016-04-15T06:00:00Z", "keyword": ["Gadolinium", "S-layer", "binding", "brine study", "chemistry", "energy", "gd3", "geothermal", "ion", "metal-binding domain", "mineral recovery", "nanosheet", "synthetic biology", "zinc", "zn2"], "landingPage": "https://gdr.openei.org/submissions/951", "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2020-01-09T00:11:19Z", "programCode": ["019:006"], "projectLead": "Holly Thomas", "projectNumber": "FY15 AOP 2514", "projectTitle": "Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines", "publisher": {"@type": "org:Organization", "name": "Lawrence Berkeley National Laboratory"}, "spatial": "{\"type\":\"Polygon\",\"coordinates\":[[[-180,-83],[180,-83],[180,83],[-180,83],[-180,-83]]]}", "title": "S-Layer Nanosheet Binding of Zn and Gd"}