{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2\u03b1 was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins.\n        \n        \n          Results\n          Targeting the Gi2\u03b1 expression by a specific ribozyme inhibited the PGF2\u03b1 -induced ERK1/2 activation in hepatocytes. On the other hand a non-cleaving form of the Gi2\u03b1 ribozyme did not significantly decrease the ERK1/2 activation. In ribozyme-treated cells the Gi2\u03b1 protein level was reduced, while the Gq\u03b1 level was not affected thus confirming the specificity of the ribozyme.\n        \n        \n          Conclusion\n          The present data suggest an important role of Gi2 in PGF2\u03b1 -induced ERK1/2 signaling in hepatocytes.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC37242/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/trhj-9ycc", "issued": "2025-07-14", "keyword": ["erk12-signaling", "gi2-protein", "hepatocytes", "nih", "prostaglandin-f2alpha"], "landingPage": "https://healthdata.gov/d/trhj-9ycc", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Evidence for the involvement of G"}