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                  <gml:beginPosition>2019-10-23</gml:beginPosition>
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The approximate collection location of the scallops in this laboratory study: Latitude: 43.593577, Longitude: -70.181889

The location of the laboratory where the experiment was conducted: Latitude: 41.737548, Longitude: -70.625153</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Dissolved Inorganic Carbon;
Abbreviation: DIC;
Unit: umol/kg;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Polypropylene bottle;
Analyzing instrument: Discrete dissolved inorganic carbon (DIC) measurement (Non-dispersive infrared, or NDIR);
Detailed sampling and analyzing information: Certified reference material was used to ensure the accuracy of the measurements. The values were within 2.7 umol/kg of the reported CRM value;
Standardization description: CRMs;
Standardization frequency: daily;
CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography.;
Preservation method: run within minutes of collection;
Preservative volume: none;
Preservative correction: none</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: pH;
Abbreviation: pH;
pH scale: Seawater scale (SWS);
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Polypropylene bottle;
Analyzing instrument: Spectrophotometer;
Detailed sampling and analyzing information: Temperature for pH analysis was ~20 to 21 C and recorded for each measurement. Tris was used to ensure accuracy with a standard deviation of 0.0014 for pH.;
Standardization description: TRIS BUFFER, Andrew Dickson Acid;
Standardization frequency: daily;
Temperature of standardization: in situ;
Temperature correction method: water bath;
At what temperature was pH reported: 20 ◦C;
Uncertainty: ± 0.0014</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Sample ID;
Abbreviation: ID;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Duration: 8 weeks;
Detailed sampling and analyzing information: Sampling id for carbon dioxide enrichment. Containers were enriched with either low, medium, or high CO2 using mass flow controllers.;
Uncertainty: 0;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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Abbreviation: Rep;
Unit: A, B, or C;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Duration: 8 weeks;
Detailed sampling and analyzing information: Replicate treatment for each carbon dioxide level. Each co2 enrichment level had 3 replicates (A, B, or C).;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
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Abbreviation: Temp;
Unit: C;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: YSI - handheld multi-parameter instrument;
Analyzing instrument: YSI - handheld multi-parameter instrument;
Duration: 8 weeks;
Detailed sampling and analyzing information: Temperature of bin on each date;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Salinity;
Abbreviation: Salinity;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Sampling instrument: YSI - handheld multi-parameter instrument;
Duration: 8 weeks;
Detailed sampling and analyzing information: Samples for salinity was taken on a YSI that uses conductivity. No units;
Uncertainty: 0.1;
Biological subject: Placopecten magellanicus;
Species ID: Placopecten magellanicus;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Temperature for pH analysis;
Abbreviation: MTpH;
Unit: C;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: Thermometer;
Analyzing instrument: Agilent Cary 100 Spectrometer;
Duration: 8 weeks;
Detailed sampling and analyzing information: Temperature that the pH was measured at. The CARY 100 is hooked up to a water bath. Temperature was measured during analysis and recorded to ensure accuracy of pH.;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Dissolved oxygen (DO);
Abbreviation: DO;
Unit: mg/l;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: YSI - handheld multi-parameter instrument;
Analyzing instrument: YSI - handheld multi-parameter instrument;
Duration: 8 weeks;
Detailed sampling and analyzing information: Dissolved oxygen in mg/L measured in each container;
Uncertainty: 0.2;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: seston in buckets;
Abbreviation: S_in;
Unit: cells per ml;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: Centrifuge tubes 50 ml;
Analyzing instrument: Accuri C6+;
Duration: 8 weeks;
Detailed sampling and analyzing information: The incoming water was natural phytoplankton cells and the addition of Tetraselmis chui (PLY429) and Chaetoceros neogracile (Chaet-B). The counts were run on a Accuri C6+ (BD Biosceince San Jose, CA, USA) after being preserved in formaldehyde. For each sample 48 ml of seawater was taken with 2 ml of formaldehyde. The range was set on high chlorophyll-a region FL3 (633 nm) to count those cells. Samples were run for 30 seconds. During 30 seconds 33 ul of sample is analyzed. The count is what was being received by the sea scallops as food. Daily variability is because of variability in the natural seston and density of the two cultures of algae. On 11/24/2019 two samples were dropped during analysis breaking the tubes preventing cell counts for Medium A and Medium B that is why no cell count is reported.;
Uncertainty: 5% of total counts;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
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          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Date;
Abbreviation: Date;
Unit: d;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: Calendar;
Analyzing instrument: calendar;
Duration: 8 weeks;
Detailed sampling and analyzing information: Day that the sample was taken.;
Uncertainty: 0;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Height of shell;
Abbreviation: H;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: digital vernier caliper;
Analyzing instrument: digital vernier caliper;
Duration: 8 weeks;
Detailed sampling and analyzing information: Digital vernier caliper were used to measure from the umbo to the outer shell margin.;
Uncertainty: 0.01;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
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          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Width of shell;
Abbreviation: W;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: digital vernier caliper;
Analyzing instrument: digital vernier caliper;
Duration: 8 weeks;
Detailed sampling and analyzing information: Using digital vernier caliper the cross section of the sea scallop was measured parallel to the umbo.;
Uncertainty: 0.01;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
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          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Thickness of shell;
Abbreviation: T;
Unit: mm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: digital vernier caliper;
Analyzing instrument: digital vernier caliper;
Duration: 8 week;
Detailed sampling and analyzing information: Digital vernier caliper was used to measure the thickenss of the shell at the center. The sea scallop was kept closed for this measurement.;
Uncertainty: 0.01;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Dry Tissue Weight;
Abbreviation: DTW;
Unit: g;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Balance;
Analyzing instrument: Balance;
Duration: 8 Weeks;
Detailed sampling and analyzing information: DTW was determined by shucking the soft tissues and drying in an oven at 60°C to constant weight. This was for 5 days.;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Dry Shell Weight;
Abbreviation: DSW;
Unit: g;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Balance;
Analyzing instrument: Balance;
Duration: 8 weeks;
Detailed sampling and analyzing information: For DSW (g), all fouling organisms and particulate material were removed with a freshwater rinse, then shells were dried to constant weight at 60°C. This took 5 days of drying.;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Total suspended material;
Abbreviation: TSM;
Unit: g/ml;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: Balance;
Analyzing instrument: balance;
Duration: 8 weeks;
Detailed sampling and analyzing information: Water samples were taken for TSM for the biodeposition method. This is the mean TSM for the biodeposition timepoint. Samples were taken every 20 minutes for a minimum of 3 hours on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. Filters were then dried at 60°C until constant weight when they were weighed again. TSM was the difference in dry filter weight- original filter weight divided by total volume of water filtered.;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Particulate inorganic material;
Abbreviation: PIM;
Unit: mg/l;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: Balance and oven;
Analyzing instrument: balance and oven;
Duration: 8 weeks;
Detailed sampling and analyzing information: PIM is the amount of particulate inorganic material in the biodeposition method. Samples were taken every 20 minutes for 3 hours during biodeposition. The mean is reported for this timeperiod. This is how much of the material was inorganic when the scallops were feeding. Samples were filtered on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. The inorganic fraction of each filter were calculated by drying the filters (60°C) until constant weight (~ 5 days) and then combusting at 450°C for 4 hours and weighing. PIM is then calculated as the material on the filter minus the initial weight of the filter divided by the volume of water filtered.;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Particulate organic material;
Abbreviation: POM;
Unit: mg/l;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: balance and oven;
Duration: 8 weeks;
Detailed sampling and analyzing information: POM is the amount of particulate organic material in the biodeposition method. Samples were taken every 20 minutes for 3 hours. This is how much of the material was organic when the scallops were feeding. Samples were filtered on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. The organic fraction of each filter were calculated by drying the filters (60°C) until constant weight (~ 5 days) and then combusting at 450°C for 4 hours and weighing. POM is then calculated as the material on the filter minus the dry filter weight divided by the volume of water filtered. The mean for biodeposition is reported.;
Biological subject: Placopecten magellanicus;
Species ID: 79718;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Day of carbon dioxide exposure;
Abbreviation: DE;
Unit: Day;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Calendar;
Duration: 8 Weeks;
Detailed sampling and analyzing information: This is the number of days that the sea scallops were exposed to carbon dioxide enrichment. The start of the experiment was 10/23/2019 day zero until December 19, 019. For day 0, 63 individualls were sampled for initial measurements. Every other sampling time point 12 individuals were sampled.;
Uncertainty: 0;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Clearance Rate;
Abbreviation: CR;
Unit: L/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Balance and oven;
Analyzing instrument: Balance;
Duration: 8 weeks;
Detailed sampling and analyzing information: Clearance rate is the amount of water moved through the sea scallop. All measurements were standardized to 0.1 g of dry tissue weight using a b factor of 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.;
Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Organic ingestion rate;
Abbreviation: OIR;
Unit: mg/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Balance;
Analyzing instrument: balance and oven;
Duration: 8 weeks;
Detailed sampling and analyzing information: The organic ingestion rate is the amount organic matter ingested. The rate was normalized to 0.1 g of dry tissue weight with a b factor 0f 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.;
Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Assimilation rate;
Abbreviation: AR;
Unit: mg/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Balance;
Analyzing instrument: balance and oven;
Duration: 8 weeks;
Detailed sampling and analyzing information: The assimilation rate is the amount of food that they ingest. All rates were normalized to 0.1 g of dry tissue weight with a b factor of 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.;
Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Assimilation efficiency;
Abbreviation: AE;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: balance and oven;
Duration: 8 weeks;
Detailed sampling and analyzing information: The assimilation efficiency that sea scallops had on acquiring food. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.;
Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Respiration rate;
Abbreviation: RR;
Unit: mg O2/ h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Loligo systems;
Analyzing instrument: Loligo systems;
Duration: 8 weeks;
Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (RR) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. An eight-channel Loligo respirometry system (Viborg, Denmark) was used to measure RR on 7 individuals with 1 chamber as the control (empty shell). RR was calculated from a linear regression applied to oxygen depletion with the background noise from the control chamber subtracted out. To ensure that scallops were not stressed from low oxygen concentrations in the chamber, recordings were stopped once oxygen saturation reached 80%. Missing values were because oxygen levels were not depleted enough to determine an accurate rate. The rate was similar to the control chamber. All rates were normalized to 0.1 g of dry tissue weight and raised to a b factor of 0.71.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Excretion Rate;
Abbreviation: ER;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Agilent Cary 100 Spectrometer;
Analyzing instrument: Agilent Cary 100 Spectrometer;
Duration: 8 weeks;
Detailed sampling and analyzing information: The same individuals used in biodeposition were used to calculate ER. Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for excretion (ER) could be measured.ER samples were taken by removing 5 mL from each chamber for ammonia-N determination after respiration was done. HACH TNT-830 test kits (equivalent EPA 350.1, EPA 351.1, and EPA 351.2) were used to determine ammonia-N with a modified protocol detailed in Pousse et al. (15). Briefly, absorbance was measured at 690 nm after 3.5 h to allow for color development. The method had a detection limit of 0.02 mg L-1. Missing values are because the excretion rate was below the method of detection. All rates were normalized to 0.1 g of dry tissue weight and raised to a b factor of 0.40.;
Uncertainty: 0.01;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Energy of Food;
Abbreviation: Food;
Unit: J/mg;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Calculated;
Duration: 8 weeks;
Detailed sampling and analyzing information: The energetic content of the food (J mg-1) obtained from Δfood= (0.632+0.086 (%C))×4.184, where %C was the POM. All blank values are because there was no AE measured for a scallop therefore the Food energy could not be calculated.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 97918</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Assimilated energy;
Abbreviation: A;
Unit: J/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Duration: 8 weeks;
Detailed sampling and analyzing information: The absorbed energy (A) is calculated as A=CR*AE*Food. Blank values are because their was no CR or AE to calculate A. For samples on December 18, 2019 samples were compromised because of the COVID-19 pandemic. The blanks for the rest of the data is because the organisms did no feed during the 5 hours the measurement was going on.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Catabolic energy;
Abbreviation: R_U;
Unit: J/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Duration: 8 weeks;
Detailed sampling and analyzing information: RR was converted to oxygen-catabolized energy (R) using 14.0 J/mg O2, ER converted to U using 24.83 J/mg NH4-N. The two were added together to get catabolic energy.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Scope For Growth;
Abbreviation: SFG;
Unit: J/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Duration: 8 weeks;
Detailed sampling and analyzing information: The scope for growth is calculated from the assimilated energy (A) minus the sum of the catabolic energy (R_U).;
Method reference: Pousse et al.;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: atomic ratio of oxygen to nitrogen;
Abbreviation: O_N;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Duration: 8 weeks;
Detailed sampling and analyzing information: The atomic ratio of RR divided by ER.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Chlorophyll-a;
Abbreviation: Chla;
Unit: ug/l;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: YSI EXO2;
Analyzing instrument: YSI EXO2;
Duration: 8 weeks;
Detailed sampling and analyzing information: Chlorphyll-a samples were taken on a YSI EXO2 with a chlorophyll sensor. Missing values are when the sensor was taken offline for calibration and maintenance.;
Uncertainty: 0.01;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Time;
Abbreviation: T;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Sampling instrument: YSI EXO2;
Analyzing instrument: YSI EXO2;
Duration: 8 weeks;
Detailed sampling and analyzing information: The time that the sample was taken. Time is reported in military time.;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Respiration rate at 12 C;
Abbreviation: R;
Unit: mg O2/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Loligo systems;
Analyzing instrument: Loligo systems;
Duration: 8 weeks;
Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (R) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. An eight-channel Loligo respirometry system (Viborg, Denmark) was used to measure R on 7 individuals with 1 chamber as the control (empty shell). Respiration rates are at 12C. The data is fitted to a power function with weight on the x axis and the R rate on the y-axis. The exponent of the power function is used in physiological measurements to scale physiological RR to body weight.;
Method reference: Pousse et al.;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: excretion rate at 12 C;
Abbreviation: E;
Unit: mg N/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Agilent Cary 100 Spectrometer;
Analyzing instrument: Agilent Cary 100 Spectrometer;
Duration: 8 weeks;
Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (E) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. ER samples were taken by removing 5 mL from each chamber for ammonia-N determination. HACH TNT-830 test kits (equivalent EPA 350.1, EPA 351.1, and EPA 351.2) were used to determine ammonia-N with a modified protocol detailed in Pousse et al. (15). Briefly, absorbance was measured at 690 nm after 3.5 h to allow for color development. The method had a detection limit of 0.02 mg/l. Excretion rates are at 12C. The data is fitted to a power function with weight on the x axis and the E rate on the y-axis. The exponent of the power function is used in physiological measurements to scale physiological ER to body weight.;
Uncertainty: 0.01 mg/l;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Clearance rate at 12 C;
Abbreviation: C;
Unit: l/h;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Duration: 8 weeks;
Detailed sampling and analyzing information: Clearance rate of sea scallops. Rates are at 12 C.;
Method reference: Pousse et al. 2020;
Biological subject: Placopecten magellanicus;
Species ID: 79718</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
          <gmd:organisationName>
            <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
          </gmd:organisationName>
          <gmd:role>
            <gmd:CI_RoleCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="custodian">custodian</gmd:CI_RoleCode>
          </gmd:role>
        </gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation id="AMS">
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_beec">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>bottle</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>bottle</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>any type of water bottle sampling device

Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be93">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>calipers</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>calipers</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>An instrument for measuring distance.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be4d">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>mass spectrometer</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>mass spectrometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>Instrument that measure the massess and relative concentrations of atoms and molecules</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_bef3">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>multi-parameter water quality sensor</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>multi-parameter water quality sensor</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>used to measure temperature, salinity, dissolved oxygen, and other parameters

Instrument used to measure temperature, salinity, dissolved oxygen, and other parameters, often used to measure water quality parameters. Can be a hand-held instrument or a sonde for unattended monitoring. Often used in coastal water monitoring stations. YSI is the preeminent brand (as of 2024).</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be94">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>scale</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>scale</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>An instrument for measuring mass or weight.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_bea3">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>thermometer</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>thermometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>generic thermometer or reversing thermometer

use thermometer when temperature is a data type, but the exact
instrument used to measure temperature is unknown</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
    </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>