{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "U.S. Geological Survey, Alaska Science Center", "hasEmail": "mailto:gs-ak_asc_datamanagers@usgs.gov"}, "description": "This release provides results of an environmental DNA (eDNA) study of fish identified in seven Alaskan lakes, three streams, and a positive control. Samples included replicates for multiple sampling dates and locations. The reference list and conserved primer sets to identify species present were developed using publicly available data (https://www.ncbi.nlm.nih.gov/genbank/). Highly conserved primers which could differentiate species of interest were developed for three portions of mtDNA genes (12S, 16S and COI).", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P9RU1RVN", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.ASC504.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_ASC504", "keyword": ["Alaska", "Animals", "Animals/Vertebrates", "Aquatic Ecosystems", "Biodiversity", "Biota", "DNA", "DNA Primers", "DNA, Mitochondrial", "Ecosystem Diversity", "Environment", "Environmental DNA", "Fish", "Genetic Diversity", "Mitochondrial DNA", "Native Species", "Species composition", "USGS:ASC504"], "modified": "2024-08-02T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-153.17, 61.43, -143.71, 70.30", "theme": ["geospatial"], "title": "Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018"}