gov.noaa.nodc:0258039 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-04-18T07:50:50Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039) 2022-08-11 publication 2022-08-09 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0258039 NCEI Archive Management System 0258039 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Jessica F. Andrade Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information resourceProvider NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider Thomas Hurst NOAA Alaska Fisheries Science Center (NMFS/AFSC) Thomas.Hurst@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Louise Copeman NOAA Alaska Fisheries Science Center (NMFS/AFSC) louise.copeman@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Jessica Andrade Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) jessica.andrade@noaa.gov https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information principalInvestigator Michelle Stowell Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) michelle.stowell@noaa.gov https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information principalInvestigator Michael L. Kent Oregon State University (OSU) michael.kent@oregonstate.edu http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information principalInvestigator Justin L. Sanders collaborator Colleen E. Al-Samarrie collaborator Hurst, Thomas P. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Copeman, Louise A. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Andrade, Jessica F. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Stowell, Michelle A. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Al-Samarrie, Colleen E. author Sanders, Justin L. author Kent, Michael L. Oregon State University (OSU) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information author NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information contributor Oregon State University (OSU) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information contributor tableDigital This dataset contains data from manipulated experimental seawater chemistry conditions and walleye pollock (Gadus chalcogrammus) larval development, swimming behavior, and lipid composition responses. The experiment took place from April 4-May 17, 2018 in the Alaska Fisheries Science Center laboratory research facilities at Hatfield Marine Science Center in Newport, Oregon. Larvae were obtained from natural spawning of laboratory-acclimated broodstock adults. Experiments occurred from fertilization to 4 weeks post-hatch at ambient (~ 425 µatm) and elevated (~ 1230 µatm) CO2 levels. This effort was conducted in support of the research objectives of the NOAA Ocean Acidification Program (OAP). To determine the effects of CO2 on behavior, growth, and health of walleye pollock larvae Funding Information: NOAA Ocean Acidification Program (Mapping OA impacts: Modeling the Multiple Action Pathways of OA effects on Alaska gadids, OAP-AFSC-FY20-001) Funding Information: NOAA's Ocean Acidification Program (Effects of OA on Alaskan groundfishes: identifying patterns and mechanisms of sensitivity and resiliency in physiological and behavioral performance, T8R3CEB-P00) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0258039 Graphic not available. PNG 0258039 NCEI ACCESSION NUMBER 2022-08-04 publication ANIMALS - INDIVIDUAL - BEHAVIOR ARAGONITE SATURATION STATE biological data DISSOLVED INORGANIC CARBON (DIC) fatty acids INDIVIDUAL FISH EXAMINATION - LENGTH INDIVIDUAL FISH EXAMINATION - WEIGHT lipids partial pressure of carbon dioxide - water pH SALINITY SPECIES IDENTIFICATION - AGE SPECIES IDENTIFICATION - DRY WEIGHT SPECIES IDENTIFICATION - LIFE STAGE SPECIES IDENTIFICATION - ORGANISM LENGTH total alkalinity WATER TEMPERATURE theme NODC DATA TYPES THESAURUS laboratory analysis microscope scale instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory experiments physical theme NODC OBSERVATION TYPES THESAURUS NOAA Alaska Fisheries Science Center Oregon State University dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Alaska Fisheries Science Center dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) project NODC PROJECT NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBONATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type 16.1_ n.7 18.1_n.7 18.1_n.9 18.2_n.6 20.4_n.6 20.5_n.3 22.5_n.3 22.5_n.6 22.6_n.3 CO2_trt DIC DWT_mean DWT_sd FFA_percent H.T_ID LabSpawn_expt MUFA_percent PL_percent PUFA_percent SB SB_size SFA_percent ST_percent TA TAG_ST TAG_percent Wild_expt _14.0 _16.0 _18.0 bottle_ID calcAr calcCO2 calcpH count_1 count_2 count_3 date dph fish_SL h2oDQ k_DWT_mean k_DWT_sd lab larv_rep life_stage lipid_ID mean_BD mean_ED mean_SL_1 mean_SL_2 mean_win.k_BD salinity sd_BD sd_ED sd_SL_1 sd_SL_2 sd_win.k_BD strikes swims tank temp time time_fed total.lipid_DWT total.lipid_indiv turns week theme Provider Variable Abbreviations MICROSCOPES > MICROSCOPES instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NOAA AFSC Laboratory Newport Oregon place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Hurst, Thomas P.; Copeman, Louise A.; Andrade, Jessica F.; Stowell, Michelle A.; Al-Samarrie, Colleen E.; Sanders, Justin L.; Kent, Michael L. (2022). Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0258039. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Hurst, T. P., Copeman, L. A., Andrade, J. F., Stowell, M. A., Al-Samarrie, C. E., Sanders, J. L., & Kent, M. L. (2021). Expanding evaluation of ocean acidification responses in a marine gadid: elevated CO2 impacts development, but not size of larval walleye pollock. Marine Biology, 168(8). https://doi.org/10.1007/s00227-021-03924-w 2021-07-06 publication International DOI Foundation (IDF) https://doi.org/10.1007/s00227-021-03924-w https://doi.org/10.1007/s00227-021-03924-w HTTPS https://doi.org/10.1007/s00227-021-03924-w journal article information Hurst, Thomas P. Alaska Fisheries Science Center, NOAA-NMFS http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Copeman, Louise A. author Andrade, Jessica F. author Stowell, Michelle A. author Al-Samarrie, Colleen E. author Sanders, Justin L. author Kent, Michael L. author Springer Science and Business Media LLC publisher documentDigital Mar Biol 168(8) ISSN 0025-3162, ISSN 1432-1793 crossReference project NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Enochs, Ian C.; Soderberg, Nash (2021). Lab-based measurements of carbonate substrate bioerosion and calcification using living date mussels (Lithophaga) collected outside Kaneohe Bay, at Site OAH-OCC-005, Hawaiian Islands, Pacific Ocean, from 2019-11-21 to 2020-01-08 (NCEI Accession 0224130). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/k43b-7176. 2021-03-30 publication International DOI Foundation (IDF) https://doi.org/10.25921/k43b-7176 NOAA National Centers for Environmental Information gov.noaa.nodc:0224130 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0224130.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0224130.html Related dataset information Enochs, Ian C. NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author Soderberg, Nash NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Feely, Richard A.; Alin, Simone R.; Hales, Burke; Johnson, Gregory C.; Juranek, Laurie W.; Byrne, Robert H.; Peterson, William T.; Goni, Miguel; Liu, Xuewu; Greeley, Dana (2015). Dissolved inorganic carbon, total alkalinity, pH, temperature, salinity and other variables collected from profile and discrete sample observations using CTD, Niskin bottle, and other instruments from R/V Wecoma in the U.S. West Coast California Current System during the 2011 West Coast Ocean Acidification Cruise (WCOA2011) from 2011-08-12 to 2011-08-30 (NCEI Accession 0123467). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5jq0xz1. 2015-01-05 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5jq0xz1 NOAA National Centers for Environmental Information gov.noaa.nodc:0123467 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123467.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123467.html Related dataset information Feely, Richard A. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Alin, Simone R. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Hales, Burke Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (OSU-CEOAS) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information author Johnson, Gregory C. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Juranek, Laurie W. Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (OSU-CEOAS) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information author Byrne, Robert H. University of South Florida (USF) rhbyrne@usf.edu https://www.usf.edu/ HTTPS University of South Florida (USF) website Institution web page information author Peterson, William T. NOAA Northwest Fisheries Science Center Newport Research Station bill.peterson@noaa.gov author Goni, Miguel Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (OSU-CEOAS) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information author Liu, Xuewu University of South Florida St. Petersburg https://www.stpetersburg.usf.edu/ HTTPS University of South Florida St. Petersburg website Institution web page information author Greeley, Dana NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Feely, Richard A.; Alin, Simone R.; Hales, Burke; Johnson, Gregory C.; Byrne, Robert H.; Peterson, William T.; Liu, Xuewu; Greeley, Dana (2015). Dissolved inorganic carbon, total alkalinity, pH on total scale and other variables collected from profile and discrete sample observations on NOAA Ship Fairweather (EXPOCODE 317W20130803) and R/V Point Sur (EXPOCODE 32P020130821) in the U.S. West Coast California Current System during the 2013 West Coast Ocean Acidification Cruise (WCOA2013) from 2013-08-03 to 2013-08-29 (NCEI Accession 0132082). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5c53hxp. 2015-11-05 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5c53hxp NOAA National Centers for Environmental Information gov.noaa.nodc:0132082 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0132082.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0132082.html Related dataset information Feely, Richard A. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Alin, Simone R. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Hales, Burke Oregon State University, College of Earth, Ocean, and Atmospheric Sciences (OSU-CEOAS) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information author Johnson, Gregory C. NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author Byrne, Robert H. University of South Florida (USF) rhbyrne@usf.edu https://www.usf.edu/ HTTPS University of South Florida (USF) website Institution web page information author Peterson, William T. NOAA Northwest Fisheries Science Center Newport Research Station bill.peterson@noaa.gov author Liu, Xuewu University of South Florida St. Petersburg https://www.stpetersburg.usf.edu/ HTTPS University of South Florida St. Petersburg website Institution web page information author Greeley, Dana NOAA Pacific Marine Environmental Laboratory (PMEL) https://www.pmel.noaa.gov/ HTTPS NOAA Pacific Marine Environmental Laboratory (PMEL) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Wieczorek, Daniel; Poach, Matthew (2014). Dissolved inorganic carbon, pH and other variables measured from laboratory experiment studies from an experimental carbonate exposure system from 2012-02-29 to 2012-05-11 (NCEI Accession 0123315). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5z03646. 2014-12-16 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5z03646 NOAA National Centers for Environmental Information gov.noaa.nodc:0123315 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123315.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123315.html Related dataset information Wieczorek, Daniel US DOC/NOAA/NMFS/Northeast Fisheries Science Center - Sandy Hook, NJ/J.J. Howard Laboratory daniel.wieczorek@noaa.gov author Poach, Matthew NOAA Northeast Fisheries Science Center (NMFS/NEFSC) author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Wieczorek, Daniel; Poach, Matthew (2014). Dissolved inorganic carbon, pH and other variables measured from laboratory experiment studies from an experimental carbonate exposure system from 2013-05-15 to 2013-07-12 (NCEI Accession 0123316). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5t72fdg. 2014-12-18 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5t72fdg NOAA National Centers for Environmental Information gov.noaa.nodc:0123316 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123316.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123316.html Related dataset information Wieczorek, Daniel US DOC/NOAA/NMFS/Northeast Fisheries Science Center - Sandy Hook, NJ/J.J. Howard Laboratory daniel.wieczorek@noaa.gov author Poach, Matthew NOAA Northeast Fisheries Science Center (NMFS/NEFSC) author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Perry, Dean M.; Redman, Dylan H.; Widman, James C., Jr.; Meseck, Shannon L.; King, Andrew L.; Pereira, Jose J. (2014). Effects of ocean acidification on growth and otolith condition of juvenile scup, Stenotomus chrysops from laboratory experiment studies from 2011-08-24 to 2011-10-19 (NCEI Accession 0117506). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5h70crk. 2014-04-28 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5h70crk NOAA National Centers for Environmental Information gov.noaa.nodc:0117506 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0117506.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0117506.html Related dataset information Perry, Dean M. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) Dean.Perry@noaa.gov http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Widman, James C., Jr. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author King, Andrew L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) andrew.king@gmail.com http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Pereira, Jose J. NOAA Northeast Fisheries Science Center (NMFS/NEFSC) author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Slesinger, Emily; Mundorff, Samantha; Laurel, Benjamin J.; Hurst, Thomas P. (2024). Growth and development impacts of laboratory experimental ocean warming and ocean acidification conditions in Pacific cod embryos and larvae from 2022-04-06 to 2022-06-02 (NCEI Accession 0295839). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/bzqv-xz96. 2024-12-11 publication International DOI Foundation (IDF) https://doi.org/10.25921/bzqv-xz96 NOAA National Centers for Environmental Information gov.noaa.nodc:0295839 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0295839.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0295839.html Related dataset information Slesinger, Emily Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Mundorff, Samantha Northeastern University http://www.northeastern.edu HTTP Northeastern University website Institution web page information author Laurel, Benjamin J. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Hurst, Thomas P. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Padilla, Dianna K.; Meseck, Shannon L. (2024). Blue mussel fertilization to early juvenile development under ocean acidification for 5 populations from Long Island Sound from 2017-04-20 to 2017-06-04 (NCEI Accession 0299732). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/614j-nz60. 2024-12-11 publication International DOI Foundation (IDF) https://doi.org/10.25921/614j-nz60 NOAA National Centers for Environmental Information gov.noaa.nodc:0299732 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0299732.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0299732.html Related dataset information Padilla, Dianna K. State University of New York at Stony Brook (SBU) https://www.stonybrook.edu/ HTTPS State University of New York at Stony Brook (SBU) website Institution web page information author Meseck, Shannon L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54. 2022-01-28 publication International DOI Foundation (IDF) https://doi.org/10.25921/fesj-kv54 NOAA National Centers for Environmental Information gov.noaa.nodc:0245952 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0245952.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0245952.html Related dataset information Pousse, Emilien author Poach, Matthew US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Sennefelder, George author Munroe, Daphne Rutgers, The State University of New Jersey author Hart, Deborah R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Hennen, Daniel R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Dixon, Mark S. author Li, Yaqin author Wikfors, Gary H. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Hubbard, William; Osborne, Kristin; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Milke, Lisa M.; Wikfors, Gary H.; Meseck, Shannon L. (2022). Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ftfq-zn70. 2022-12-23 publication International DOI Foundation (IDF) https://doi.org/10.25921/ftfq-zn70 NOAA National Centers for Environmental Information gov.noaa.nodc:0276024 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0276024.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0276024.html Related dataset information Pousse, Emilien US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Poach, Matthew US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Sennefelder, George US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Hubbard, William Massachusetts Maritime Academy author Osborne, Kristin Massachusetts Maritime Academy author Munroe, Daphne Rutgers, The State University of New Jersey author Hart, Deborah R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Hennen, Daniel R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Dixon, Mark S. author Li, Yaqin US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Milke, Lisa M. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Wikfors, Gary H. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Gurr, Samuel J.; Meseck, Shannon L.; McFarland, Katherine (2025). Eastern oyster larva exposed to mutliple drivers of aragonite undersaturation from 2021-04-29 to 2021-05-21 (NCEI Accession 0300468). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/2ycy-7409. 2025-01-15 publication International DOI Foundation (IDF) https://doi.org/10.25921/2ycy-7409 NOAA National Centers for Environmental Information gov.noaa.nodc:0300468 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0300468.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0300468.html Related dataset information Gurr, Samuel J. NOAA Northeast Fisheries Science Center (NMFS/NEFSC) author Meseck, Shannon L. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author McFarland, Katherine US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project eng; USA utf8 environment oceans biota -122.76 -122.76 48.136 48.136 2018-04-04 2018-05-17 This data package (Submission ID: BF1537UYC) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. Scientific Abstract: Responses of marine populations to climate conditions reflect the integration of a suite of complex and interrelated physiological and behavioral responses at the individual level. Many of these responses are not immediately reflected in changes to survival, but may impact growth or survival at later life stages. Understanding the broad range of impacts of rising CO2 concentrations on marine fishes is critical to predicting the consequences of ongoing ocean acidification. Walleye pollock (Gadus chalcogrammus) support the largest single-species fishery in the world and provide a critical forage base throughout north Pacific ecosystems. Previous studies of high CO2 effects on early life stages of walleye pollock have suggested a general resiliency in this species, but those studies focused primarily on growth and survival rates. Here, we expand on earlier studies with an independent experiment focused on walleye pollock larval development, swimming behavior, and lipid composition from fertilization to 4&nbsp;weeks post-hatch at ambient (~ 425&nbsp;µatm) and elevated (~ 1230&nbsp;µatm) CO2 levels. Consistent with previous observations, size metrics of walleye pollock were generally insensitive to CO2 treatment. However, 4-week post-hatch larvae had significantly reduced rates of swim bladder inflation. A modest change in the swimming behavior of post-feeding larvae was observed at four, but not at 2&nbsp;weeks post-hatch. Although there were no differences in overall lipid levels between CO2 treatments, the ratio of energy storage lipids (triacylglycerols) to structural membrane lipids (sterols) was lower among larvae reared at high CO2 levels. Although we observed higher survival to 4&nbsp;weeks post-hatch among fish reared at high CO2 levels, the observations of reduced swim bladder inflation rates and changes in lipid cycling suggest the presence of sub-lethal effects of acidification that may carry over and manifest in later life stages. These observations support the continued need to evaluate the impacts of ocean acidification on marine fishes across a wide range of traits and life stages with replicated, independent experiments. NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Excel 0.196 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0258039.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/0258039 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/258039 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/258039 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0206/0258039/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0258039 v1.1 was published. 2022-08-11T20:57:34Z NCEI Accession 0258039 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0258039/1.1 HTTPS NCEI Accession 0258039 v1.1 published 2022-08-11T20:57:34Z download nonGeographicDataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer); Preservation method: saturated HgCl2; Preservative correction: none; Uncertainty: <0.05%; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska Fairbanks Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity); CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: saturated HgCl2; Preservative correction: none; Uncertainty: <0.5%; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pH; Abbreviation: calcpH; pH scale: seawater; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Sampling instrument: Siphoning tube; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 5.9±0.4°C; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: calcCO2; Unit: µatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Sampling instrument: Siphoning tube; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; Quality flag convention: "h2oDQ" reflects reliability of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: water temperature; Abbreviation: temp; Unit: °C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Analyzing instrument: various; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: water salinity; Abbreviation: salinity; Unit: ppt; Observation type: Laboratory experiment; Analyzing instrument: Yellow Springs International meter - Model YSI 85; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: aragonite saturation state; Abbreviation: calcAr; Unit: omega; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: calculated using CO2sys program; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: data quality flag for CO2 system measures; Abbreviation: h2oDQ; Unit: coded: 1=good; 2=questionable/outlier/estimated; 3=known error; Observation type: Laboratory experiment; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: number of swimming movements by an individual; Abbreviation: swims; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of distinct forward swims within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: number of turns made by an individual; Abbreviation: turns; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of distinct turns within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: number of food strikes made by an individual; Abbreviation: strikes; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: count of food strikes within a one-minute observation period occurring thirty minutes after introducing food; Replicate information: up to 6 fish were observed from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: swim bladder presence/absence; Abbreviation: SB; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: coded as: 1 = swim bladder present, or 0 = swim bladder absent; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA Parameter or Variable: swim bladder size; Abbreviation: SB_size; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: maximum length of the swim bladder; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA Parameter or Variable: fish standard length; Abbreviation: fish_SL; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Michael L. Kent; Researcher institution: Department of Microbiology, Oregon State University, Corvallis, OR, USA Parameter or Variable: mean standard length 1; Abbreviation: mean_SL_1; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean standard length 1; Abbreviation: sd_SL_1; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean body depth; Abbreviation: mean_BD; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean body depth; Abbreviation: sd_BD; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean eye diameter; Abbreviation: mean_ED; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean eye diameter; Abbreviation: sd_ED; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean body condition - expressed as winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: mean_win.k_BD; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean body condition - expressed as winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: sd_win.k_BD; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean standard length 2; Abbreviation: mean_SL_2; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean standard length 2; Abbreviation: sd_SL_2; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean dry weight; Abbreviation: DWT_mean; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean dry weight; Abbreviation: DWT_sd; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Analyzing instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean body condition - expressed as fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: k_DWT_mean; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean body condition - expressed as fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: k_DWT_sd; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50°C for at least 48 hours prior to measurement; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage of total lipids composed of triacylglycerols in the lipid sample; Abbreviation: TAG_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a triacylglycerol standard purified from gadid liver; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage of total lipids composed of free fatty acids in the lipid sample; Abbreviation: FFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) palmitic acid standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage of total lipids composed of sterols; Abbreviation: ST_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) cholesterol standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage of total lipids composed of polar lipids in the lipid sample; Abbreviation: PL_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) L-alpha-phosphatidylcholine standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: ratio of triacylglycerol to sterol in lipid sample; Abbreviation: TAG_ST; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area was used with a calibration curve based on a commercial (Sigma) L-alpha-phosphatidylcholine standard; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: total lipids per dry weight of lipid sample; Abbreviation: total.lipid_DWT; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: Sum of all lipid class amounts in the lipid sample (TAG, FFA, ST, PL, in ug) divided by an estimate of the lipid sample dry weight (mg). Lipid sample dry weight estimated by multiplying mean dry weight per larva by number of larvae in lipid sample.; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: total lipids per individual larva; Abbreviation: total.lipid_indiv; Unit: µg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: sum of all lipid class amounts in the lipid sample (TAG, FFA, ST, PL, in ug) divided by number of larvae in lipid sample; Analyzing instrument: Iatroscan TLC-FID (MK-6s, Iatron Laboratories) with PeakSimple integration software (ver. 3.67, SRI Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. Lipid classes were determined using thin layer chromatography with flame ionization detection, by micropipetting lipid extracts onto duplicate silica-coated glass rods, developing the rods in a three-stage solvent system to separate lipid classes, scanning the rods on a MK-6s Iatroscan TLC-FID, and integrating the resulting chromatograms using integration software with a calibration curve.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Lu et al. (2008) Can J Fish Aquat Sci 65:2233-2241; Copeman et al. (2017) Mar Ecol Prog Ser 566:183-198.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 14:0 (i.e., myristic acid) in the lipid sample; Abbreviation: _14.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 14:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 16:0 (i.e., palmitic acid) in the lipid sample; Abbreviation: _16.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 16:1n-7 (i.e., palmitoleic acid) in the lipid sample; Abbreviation: 16.1_ n.7; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:0 (i.e., stearic acid) in the lipid sample; Abbreviation: _18.0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:1n-9 (i.e., oleic acid) in the lipid sample; Abbreviation: 18.1_n.9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:1n-7 (i.e., vaccenic acid) in the lipid sample; Abbreviation: 18.1_n.7; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 18:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 18:2n-6 (i.e., linoleic acid) in the lipid sample; Abbreviation: 18.2_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 20:4n-6 (i.e., arachidonic acid) in the lipid sample; Abbreviation: 20.4_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Calculation method and parameters: chromatogram peak area for 20:4n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 20:5n-3 (i.e., eicosapentaenoic acid) in the lipid sample; Abbreviation: 20.5_n.3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 20:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:5n-6 (i.e., osbond acid) in the lipid sample; Abbreviation: 22.5_n.6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:5n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:5n-3 (i.e., clupanodonic acid) in the lipid sample; Abbreviation: 22.5_n.3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of 22:6n-3 (i.e., docosahexaenoic acid) in the lipid sample; Abbreviation: 22.6_n.3; Unit: none; Observation type: none; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: chromatogram peak area for 22:6n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of saturated fatty acids in the lipid sample; Abbreviation: SFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all saturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of monounsaturated fatty acids in the lipid sample; Abbreviation: MUFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all monounsaturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: percentage (by weight) of total fatty acids composed of polyunsaturated fatty acids in the lipid sample; Abbreviation: PUFA_percent; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Calculation method and parameters: sum of percentages of all polyunsaturated fatty acids in the lipid sample; Analyzing instrument: HP 7890A GC-FID with DB-WAX column and 7693 autosampler (Agilent Technologies, Inc.), and Chem Station software (ver A.01.02, Agilent Technologies, Inc.); Detailed sampling and analyzing information: Following 2 and 4 weeks of larval culture, at least 50 larvae from each replicate tank were pipetted onto pre-combusted glass fiber filters and stored in chloroform at -20 degrees centigrade until lipid extraction. Lipids were extracted by homogenizing each sample in a 2:1 (v/v) solution of chloroform and methanol and separating the solution to obtain the neutral lipid chloroform layer over four washes. A known quantity of methyl tricosanoate (23:0) was added to the lipid extracts, which were derivatized to form fatty acid methyl esters using acid transesterifcation with sulfuric acid in methanol (Hilditch reagent). Derivatives were analyzed with gas chromatography and the resulting chromatograms were analyzed with integration software.; Replicate information: 51 to 59 larvae were sampled and pooled into a single lipid sample from each treatment replicate on each sampling date.; Method reference: Parrish (1987) Can J Fish Aquat Sci 44:722-731; Folch et al. (1956) J Biol Chem 226:497-509; Budge et al. (2006) Mar Mammal Sci 22:759-801.; Biological subject: walleye pollock; Species ID: Gadus chalcogrammus; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: categorical life stage of organism; Abbreviation: life_stage; Unit: none; Detailed sampling and analyzing information: coded as "Egg", "Larv", or "Adult" Parameter or Variable: sampling date; Abbreviation: date; Unit: none Parameter or Variable: time of day of sample collection; Abbreviation: time; Unit: none Parameter or Variable: seawater sample identification; Abbreviation: bottle_ID; Unit: none Parameter or Variable: laboratory categorical identifier of seawater sample location; Abbreviation: lab; Unit: none; Detailed sampling and analyzing information: coded as "CR2", "3", or "NAL" to distinguish between distinct locations of seawater samples Parameter or Variable: CO2 treatment; Abbreviation: CO2_trt; Unit: none; Detailed sampling and analyzing information: categorical identifier coded as "Ambient" or "High" CO2 treatment Parameter or Variable: binary identifier of experimental 'source' of seawater sample; Abbreviation: Wild_expt; Unit: none; Detailed sampling and analyzing information: coded as: 1 = water from experiment that used eggs and larvae reared from wild-caught eggs, or 0 = water from experiment that used eggs and larvae reared from broodstock Parameter or Variable: binary identifier of experimental 'source' of seawater sample; Abbreviation: LabSpawn_expt; Unit: none; Detailed sampling and analyzing information: coded as: 1 = water from experiment that used eggs and larvae reared from broodstock, or 0 = water from experiment that used eggs and larvae reared from wild-caught eggs Parameter or Variable: categorical identifier of seawater sample source; Abbreviation: H.T_ID; Unit: none; Detailed sampling and analyzing information: coded as "1_1", "1_2", "CA_1", "CA_2", "A5", "A6", "L5", "L6", "H1", "H2", "H3", or "H4" to distinguish between distinct sources of seawater samples Parameter or Variable: larval age in days post hatch when data were collected; Abbreviation: dph; Unit: none Parameter or Variable: binary identifier of larval age in weeks (post hatch) when data were collected; Abbreviation: week; Unit: none; Detailed sampling and analyzing information: coded as: 1 = 14+ dph, or 2 = 28+ dph Parameter or Variable: experimental rearing tank identifier; Abbreviation: tank; Unit: none; Detailed sampling and analyzing information: coded as "3", "4", "5", "6", "11", "12", or "13" to distinguish between distinct experimental rearing tanks Parameter or Variable: time of day when fish were given food; Abbreviation: time_fed; Unit: none Parameter or Variable: identifier of a tank's larval replicate for each sampling age; Abbreviation: larv_rep; Unit: none Parameter or Variable: number of larvae used to calculate the means ± standard deviations of each treatment replicate for standard length, body depth, eye diameter, and mean body condition expressed as a winsorized fractional deviation from the ontogenetic relationship between SL & BD; Abbreviation: count_1; Unit: none Parameter or Variable: number of larvae used to calculate the means ± standard deviations of each treatment replicate for standard length, dry weight, and mean body condition expressed as a fractional deviation from the ontogenetic relationship between SL & DWT; Abbreviation: count_2; Unit: none Parameter or Variable: lipid sample identification; Abbreviation: lipid_ID; Unit: none Parameter or Variable: number of larvae used to obtain lipid/fatty acid data; Abbreviation: count_3 asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian laboratory analysis laboratory analysis use as an observation type microscope microscope instrument is used in lab analyses A microscope is an instrument used to see objects that are too small for the naked eye. scale scale An instrument for measuring mass or weight.