{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Michael Madden", "hasEmail": "mailto:madden.michael@epa.gov"}, "description": "Figure 1. Comparison of cell viability in response to exposure of cells to oleic acid (in ethanol), a hydroxy-metabolite, or methylated oleic acid as percent of the vehicle control response. \nFigure 2. Mitochondrial  stress test extracellular flux using comparison of cellular oxygen consumption rate measures.\nFigure 3. Soluble mediator release comparison of cells exposed to oleic acid and its metabolites. \nFigure 4. Cellular iron-uptake following incubation with fatty acids.\nSupplement 2A. Lactate dehydrogenase  activity release ( a measure of cell viability) is dose- and time-dependent.\nSupplement 2B. Oleic acid cellular association differs with vehicle utilized. \n\nThis dataset is associated with the following publication:\nBass, V., J. Soukup, A. Ghio, and M. Madden. Oleic Acid and Derivatives Affect Human Endothelial Mitochondrial Function Cell and Vasoactive Mediator Production.   Lipids in Health and Disease. BioMed Central Ltd, London,  UK, 19(1): 128, (2020).", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Figure%201%20Scihub%20data%20cell%20viability%209april2019.pdf", "mediaType": "application/pdf", "title": "Figure 1 Scihub data cell viability 9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Figure%202%20Scihub%20data%20seahorse%20%20assay%209april2019.pdf", "mediaType": "application/pdf", "title": "Figure 2 Scihub data seahorse  assay 9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Figure%203%20Scihub%20data%20PGF2a%20%20%20ET1%20%209april2019.pdf", "mediaType": "application/pdf", "title": "Figure 3 Scihub data PGF2a   ET1  9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Figure%204%20Scihub%20data%20genes%20changes%209april2019.pdf", "mediaType": "application/pdf", "title": "Figure 4 Scihub data genes changes 9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Figure%205%20Scihub%20data%20iron%20%209april2019.pdf", "mediaType": "application/pdf", "title": "Figure 5 Scihub data iron  9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Supplement%20Figure%202AScihub%20data%20viability%20BSA%20vehicle%209april2019.pdf", "mediaType": "application/pdf", "title": "Supplement Figure 2AScihub data viability BSA vehicle 9april2019.pdf"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1503700/Supplement%20Figure%202B%20Scihub%20data%2014C_oleic%20uptake%209april2019.pdf", "mediaType": "application/pdf", "title": "Supplement Figure 2B Scihub data 14C_oleic uptake 9april2019.pdf"}], "identifier": "https://doi.org/10.23719/1503700", "keyword": ["air pollutant", "oleic acid", "vascular toxicity"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2019-04-09", "programCode": ["020:094"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1186/s12944-020-01296-6"], "rights": null, "title": "Effects of Oleic Acid on Human Endothelal Cells"}