{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["006:55"], "contactPoint": {"fn": "Samuel P. Forry", "hasEmail": "mailto:samuel.forry@nist.gov"}, "description": "This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.", "distribution": [{"description": "R code for analysis and figure generation.", "downloadURL": "https://data.nist.gov/od/ds/mds2-3092/resources.zip", "format": "R markdown document.", "mediaType": "application/zip", "title": "resources"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/00README.rtf", "mediaType": "application/rtf", "title": "00README"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/DataAnalysis.Rmd", "mediaType": "application/octet-stream", "title": "DataAnalysis"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/ProcessingRawReads.Rmd", "mediaType": "application/octet-stream", "title": "ProcessingRawReads"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3Silvav132.txt", "mediaType": "text/plain", "title": "dada2_output/v3_taxa_tab3Silvav132"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3Silvav138.txt", "mediaType": "text/plain", "title": "dada2_output/v3_taxa_tab3Silvav138"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv3_taxa_tab3rdp18.txt", "mediaType": "text/plain", "title": "dada2_output/v3_taxa_tab3rdp18"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3Silvav132.txt", "mediaType": "text/plain", "title": "dada2_output/v4_taxa_tab3Silvav132"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3Silvav138.txt", "mediaType": "text/plain", "title": "dada2_output/v4_taxa_tab3Silvav138"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/dada2_output%2Fv4_taxa_tab3rdp18.txt", "mediaType": "text/plain", "title": "dada2_output/v4_taxa_tab3rdp18"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-3092/raw_reads.zip", "mediaType": "application/zip", "title": "raw_reads"}], "identifier": "ark:/88434/mds2-3092", "issued": "2023-11-16", "keyword": ["design of experiments", "full factorial", "metagenomic sequencing", "microbiome"], "landingPage": "https://data.nist.gov/od/id/mds2-3092", "language": ["en"], "license": "https://www.nist.gov/open/license", "modified": "2023-10-05 00:00:00", "programCode": ["006:045"], "publisher": {"@type": "org:Organization", "name": "National Institute of Standards and Technology"}, "theme": ["Bioscience:Microbial measurements"], "title": "A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements"}