{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["006:55"], "contactPoint": {"fn": "Jeffrey W. Hudgens", "hasEmail": "mailto:jeffrey.hudgens@nist.gov"}, "description": "This tutorial provides  mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to \u00b10.1 oC are also included in this tutorial.", "distribution": [{"accessURL": "https://doi.org/10.18434/M32151", "title": "DOI Access for Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry"}, {"description": "STL Files for parts used to construct the Multi-channel Temperature Controller", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z", "format": "7Z", "mediaType": "application/octet-stream", "title": "STL Files for parts used to construct the Multi-channel Temperature Controller"}, {"description": "This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column.", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx", "format": "word processing document", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"}, {"description": "This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z", "mediaType": "application/octet-stream", "title": "Multi-Channel Valve Controller STL files"}, {"description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller.", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf", "mediaType": "application/pdf", "title": "Multi-Channel Valve Controller"}, {"description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf", "mediaType": "application/pdf", "title": "Construction information for the Multi-channel Temperature Controller"}, {"description": "This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf", "mediaType": "application/pdf", "title": "HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"}, {"description": "This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf.", "downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z", "format": "7Z", "mediaType": "application/octet-stream", "title": "STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx.sha256", "mediaType": "text/plain", "title": "SHA256 File for ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z.sha256", "mediaType": "text/plain", "title": "SHA256 File for STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf.sha256", "mediaType": "text/plain", "title": "SHA256 File for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z.sha256", "mediaType": "text/plain", "title": "SHA256 File for STL Files for parts used to construct the Multi-channel Temperature Controller"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf.sha256", "mediaType": "text/plain", "title": "SHA256 File for Construction information for the Multi-channel Temperature Controller"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z.sha256", "mediaType": "text/plain", "title": "SHA256 File for Multi-Channel Valve Controller STL files"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf.sha256", "mediaType": "text/plain", "title": "SHA256 File for Multi-Channel Valve Controller"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf", "mediaType": "application/pdf"}, {"downloadURL": "https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf.sha256", "mediaType": "text/plain"}], "identifier": "ark:/88434/mds2-2151", "issued": "2020-04-14", "keyword": ["hydrogen-deuterium exchange", "liquid chromatography", "mass spectrometry", "peptide", "precision", "protein", "proteolysis", "proteomics", "reference material", "temperature control."], "landingPage": "https://data.nist.gov/od/id/mds2-2151", "language": ["en"], "license": "https://www.nist.gov/open/license", "modified": "2019-02-20 00:00:00", "programCode": ["006:045"], "publisher": {"@type": "org:Organization", "name": "National Institute of Standards and Technology"}, "theme": ["Bioscience:Bioprocessing", "Bioscience:Proteomics", "Chemistry:Analytical chemistry", "Metrology:Amount of substance", "Physics:Biological physics"], "title": "Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry"}