{"@type": "dcat:Dataset", "accessLevel": "public", "accrualPeriodicity": "irregular", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "GeneLab Outreach", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov"}, "description": "The present study concerns the effects of 21 days of sustained recumbence (bedrest) and hypoxia alone and in combination on skeletal muscle microRNA expression. 14 male subjects participated in 3 experimental campaigns in a counterbalanced fashion: normoxic bedrest (NBR) hypoxic bedrest (HBR) and hypoxic ambulatory confinement (HAMB) both hypoxic conditions with FO2 = 0.141 and PIO2 = 90  xc2 xb1 0.4 mmHg equivalent to an altitude of  xe2 x89 x88 4000 m). Each intervention (bedrest or ambulatory confinement) lasted 21 days and the interventions were separated by a 4-month wash-out/recovery period. The order in which each subject undertook the interventions is denoted   Intervention_order   Throughout both bedrest interventions each subject remained in a horizontal position at all times. He was allowed one pillow for the head and to occasionally lean on an elbow while eating or being transferred to a gurney. Muscular exercise was prohibited. During the HAMB confinement each subject was allowed to move freely within the hypoxic area. To mimic a normal level of physical activity subjects performed 30 minutes of low-to-moderate-intensity exercise twice daily. Muscle samples from Vastus lateralis.", "distribution": [{"@type": "dcat:Distribution", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-198", "format": "HTML", "mediaType": "text/html", "title": "Effects of bedrest and hypoxia on human skeletal muscle miRNA expression"}], "identifier": "nasa_genelab_GLDS-198_63bx-knk2", "issued": "2021-05-21", "keyword": ["bed-rest", "experimental-campaign", "labeling", "normalization-data-transformation", "nucleic-acid-hybridization-and-data-collection", "rna-extraction", "sample-collection", "sample-collection-protocol", "sampling-time", "scan-protocol", "treatment", "treatment-protocol"], "landingPage": "https://data.nasa.gov/dataset/effects-of-bedrest-and-hypoxia-on-human-skeletal-muscle-mirna-expression", "license": "http://www.usa.gov/publicdomain/label/1.0/", "modified": "2025-04-23", "programCode": ["026:005"], "publisher": {"@type": "org:Organization", "name": "National Aeronautics and Space Administration"}, "theme": ["Earth Science"], "title": "Effects of bedrest and hypoxia on human skeletal muscle miRNA expression"}