gov.noaa.nodc:0123400 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-07-25T06:02:20Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400) 2015-01-13 publication 2021-10-07 revision 2014-12-02 creation International DOI Foundation (IDF) 10.7289/v59884zw NOAA National Centers for Environmental Information gov.noaa.nodc:0123400 NCEI Archive Management System 0123400 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Shannon L. Meseck NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) Shannon.Meseck@noaa.gov http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information resourceProvider NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information resourceProvider Robert J. Foy NOAA Alaska Fisheries Science Center (NMFS/AFSC) Robert.Foy@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator W. Christopher Long NOAA Alaska Fisheries Science Center (NMFS/AFSC) chris.long@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Katherine Swiney NOAA Alaska Fisheries Science Center (NMFS/AFSC) Katherine.Swiney@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Shannon Meseck NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) Shannon.Meseck@noaa.gov http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information collaborator Jennifer H. Alix collaborator Gary H. Wikfors collaborator Meseck, Shannon L. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Alix, Jennifer H. author Swiney, Katherine M. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Long, W. Christopher NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Wikfors, Gary H. author Foy, Robert J. NOAA Alaska Fisheries Science Center Robert.Foy@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information contributor tableDigital Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions. We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water. Total counts of hemocytes did not vary among experimental control and treatments; however, there was a significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment. Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment. These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH. There was no significant difference in pHi within hyalinocytes among pH levels, with apparent regulation to a mean pHi of 7.24, significantly lower than the external environment. In contrast, there was a significant difference between treatments in pHi of the semi-granular+granular cells. These findings suggest that future, predicted levels of ocean acidification may affect the defense cells of Tanner crabs, possibly making them more susceptible to other stressors in the environment. To determine if hemocytes were effect during ocean acidification Funding Information: NOAA Ocean Acidification Program (Effects of ocean acidification on federally managed crab species in Alaska, OAPFY13.03.AFSC.001) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded NCEI Accession 0123400 was revised and a new version of the archival package was published. Updates to existing archival packages may provide additional files or replace obsolete files. The latest version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. Please see journal.txt in the /about directory for additional details on changes made. https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0123400 Preview graphic PNG 0123400 NCEI ACCESSION NUMBER 2021-09-27 publication biological data CELL COUNTS CRUSTACEANS MICROBIOLOGY pH SHELLFISH theme NODC DATA TYPES THESAURUS Flow Cytometer instrument NODC INSTRUMENT TYPES THESAURUS biological laboratory analyses theme NODC OBSERVATION TYPES THESAURUS NOAA Alaska Fisheries Science Center dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Northeast Fisheries Science Center, Milford Laboratory dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS NODC Ocean Acidification Scientific Data Stewardship (OADS) Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS Gulf of Alaska North Pacific Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > MOLLUSKS EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type external hemolymph pH hemocytes internal cell pH intracellular pH theme Provider Keywords % Dead Cell SGC+GC % Dead HC % Phagocytosis Average pH Calculated HC pHi Calculated SGC+GC pHi Dead Cell HC Dead Cell SGC+GC Hemolymph pHe Raw data SNARF-5F HC FL2-H to calculate internal cell pH Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH Total Cell HC Total Cell SGC+GC theme Provider Variable Abbreviations FLOW CYTOMETRY instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN > GULF OF ALASKA place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Gulf of Alaska North Pacific Ocean place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, W. Christopher; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v59884zw. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Meseck, S. L., Alix, J. H., Swiney, K. M., Long, W. C., Wikfors, G. H., & Foy, R. J. (2016). Ocean Acidification Affects Hemocyte Physiology in the Tanner Crab (Chionoecetes bairdi). PLOS ONE, 11(2), e0148477. https://doi.org/10.1371/journal.pone.0148477 2016-02-09 publication International DOI Foundation (IDF) https://doi.org/10.1371/journal.pone.0148477 https://doi.org/10.1371/journal.pone.0148477 HTTPS https://doi.org/10.1371/journal.pone.0148477 journal article information Meseck, Shannon L. author Alix, Jennifer H. author Swiney, Katherine M. author Long, W. Christopher author Wikfors, Gary H. author Foy, Robert J. author Melzner, Frank editor Public Library of Science (PLoS) publisher documentDigital PLoS ONE 11(2) ISSN 1932-6203, p. e0148477 crossReference project NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Perry, Dean M.; Redman, Dylan H.; Widman, James C., Jr.; Meseck, Shannon L.; King, Andrew L.; Pereira, Jose J. (2014). Effects of ocean acidification on growth and otolith condition of juvenile scup, Stenotomus chrysops from laboratory experiment studies from 2011-08-24 to 2011-10-19 (NCEI Accession 0117506). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5h70crk. 2014-04-28 publication International DOI Foundation (IDF) https://doi.org/10.7289/v5h70crk NOAA National Centers for Environmental Information gov.noaa.nodc:0117506 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0117506.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0117506.html Related dataset information Perry, Dean M. NOAA Northeast Fisheries Science Center, Milford Laboratory Dean.Perry@noaa.gov http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Widman, James C., Jr. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author King, Andrew L. NOAA Northeast Fisheries Science Center, Milford Laboratory andrew.king@gmail.com http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Pereira, Jose J. NOAA Northeast Fisheries Science Center author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Slesinger, Emily; Mundorff, Samantha; Laurel, Benjamin J.; Hurst, Thomas P. (2024). Growth and development impacts of laboratory experimental ocean warming and ocean acidification conditions in Pacific cod embryos and larvae from 2022-04-06 to 2022-06-02 (NCEI Accession 0295839). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/bzqv-xz96. 2024-12-11 publication International DOI Foundation (IDF) https://doi.org/10.25921/bzqv-xz96 NOAA National Centers for Environmental Information gov.noaa.nodc:0295839 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0295839.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0295839.html Related dataset information Slesinger, Emily Cooperative Institute for Marine Ecosystem and Resource Studies https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Mundorff, Samantha Northeastern University http://www.northeastern.edu HTTP Northeastern University website Institution web page information author Laurel, Benjamin J. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Hurst, Thomas P. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Padilla, Dianna K.; Meseck, Shannon L. (2024). Blue mussel fertilization to early juvenile development under ocean acidification for 5 populations from Long Island Sound from 2017-04-20 to 2017-06-04 (NCEI Accession 0299732). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/614j-nz60. 2024-12-11 publication International DOI Foundation (IDF) https://doi.org/10.25921/614j-nz60 NOAA National Centers for Environmental Information gov.noaa.nodc:0299732 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0299732.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0299732.html Related dataset information Padilla, Dianna K. State University of New York at Stony Brook https://www.stonybrook.edu/ HTTPS State University of New York at Stony Brook (SBU) website Institution web page information author Meseck, Shannon L. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54. 2022-01-28 publication International DOI Foundation (IDF) https://doi.org/10.25921/fesj-kv54 NOAA National Centers for Environmental Information gov.noaa.nodc:0245952 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0245952.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0245952.html Related dataset information Pousse, Emilien author Poach, Matthew NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Sennefelder, George author Munroe, Daphne Rutgers, The State University of New Jersey author Hart, Deborah R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Hennen, Daniel R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Dixon, Mark S. author Li, Yaqin author Wikfors, Gary H. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Hubbard, William; Osborne, Kristin; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Milke, Lisa M.; Wikfors, Gary H.; Meseck, Shannon L. (2022). Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ftfq-zn70. 2022-12-23 publication International DOI Foundation (IDF) https://doi.org/10.25921/ftfq-zn70 NOAA National Centers for Environmental Information gov.noaa.nodc:0276024 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0276024.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0276024.html Related dataset information Pousse, Emilien NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Poach, Matthew NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Redman, Dylan H. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Sennefelder, George NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Hubbard, William Massachusetts Maritime Academy author Osborne, Kristin Massachusetts Maritime Academy author Munroe, Daphne Rutgers, The State University of New Jersey author Hart, Deborah R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Hennen, Daniel R. US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory http://www.nefsc.noaa.gov/nefsc/woodshole HTTP US DOC/NOAA/NMFS/Northeast Fisheries Science Center/Woods Hole Laboratory website Institution web page information author Dixon, Mark S. author Li, Yaqin NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Milke, Lisa M. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Wikfors, Gary H. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author Meseck, Shannon L. NOAA Northeast Fisheries Science Center, Milford Laboratory http://nefsc.noaa.gov/nefsc/Milford/ HTTP NOAA Northeast Fisheries Science Center, Milford Laboratory (NMFS/NEFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Meseck, Shannon L.; Gurr, Samuel J.; McFarland, Katherine; Padilla, Dianna K. (2025). Dissolved inorganic carbon, pH, alkalinity, temperature, salinity, and biological response variables (growth, survival, deformities) collected from laboratory experiments for the research project (OAP- Research Organization Registry 02bfn4816; grant # 21268) for bay scallop larvae, Argopecten irradians, for 2 generations conducted at the NOAA Milford Laboratory in Milford, Connecticut from 2021-07-26 to 2022-08-30 (NCEI Accession 0302112). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/92td-sm58. 2025-03-05 publication International DOI Foundation (IDF) https://doi.org/10.25921/92td-sm58 NOAA National Centers for Environmental Information gov.noaa.nodc:0302112 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0302112.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0302112.html Related dataset information Meseck, Shannon L. NOAA Northeast Fisheries Science Center author Gurr, Samuel J. NRC Research Associate author McFarland, Katherine NOAA Office for Coastal Management https://coast.noaa.gov/ HTTPS NOAA Office for Coastal Management (OCM) website Institution web page information author Padilla, Dianna K. State University of New York at Stony Brook https://www.stonybrook.edu/ HTTPS State University of New York at Stony Brook (SBU) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Meseck, Shannon L.; McFarland, Katherine; Gurr, Samuel J.; Wikfors, Gary H. (2025). Biological response variables of hemolymph (cell viability, mtROS, and mitochondrial membrane potential) and dissolved inorganic carbon, pH, alkalinity, temperature, salinity, and collected from laboratory experiments for the research project (OAP-Research Organization Registry 02bfn4816) grant # 21268) for adult bay scallop, Argopecten irradians from 2023-05-02 to 2023-05-16 (NCEI Accession 0306498). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/wq5f-6k66. 2025-07-24 publication International DOI Foundation (IDF) https://doi.org/10.25921/wq5f-6k66 NOAA National Centers for Environmental Information gov.noaa.nodc:0306498 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306498.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306498.html Related dataset information Meseck, Shannon L. NOAA Northeast Fisheries Science Center author McFarland, Katherine National Ocean Service, Office for Coastal Management author Gurr, Samuel J. NRC Post-doc author Wikfors, Gary H. NOAA Northeast Fisheries Science Center author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Meseck, Shannon L.; McFarland, Katherine; Gurr, Samuel J.; Padilla, Dianna K. (2026). Dissolved inorganic carbon, pH, alkalinity, temperature, salinity, and biological response variables (growth and tissue allocation) of juveniles and adults collected from laboratory experiments for the research project (OAP- Research Organization Registry 02bfn4816; grant # 21268) for bay scallops, Argopecten irradians, at the NOAA Milford Laboratory in Milford, Connecticut from 2021-08-20 to 2022-06-30 (NCEI Accession 0315508). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/j79q-d927. 2026-06-06 publication International DOI Foundation (IDF) https://doi.org/10.25921/j79q-d927 NOAA National Centers for Environmental Information gov.noaa.nodc:0315508 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0315508.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0315508.html Related dataset information Meseck, Shannon L. NOAA Northeast Fisheries Science Center author McFarland, Katherine NOAA Northeast Fisheries Science Center author Gurr, Samuel J. NOAA Northeast Fisheries Science Center author Padilla, Dianna K. State University of New York at Stony Brook https://www.stonybrook.edu/ HTTPS State University of New York at Stony Brook (SBU) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project eng; USA utf8 environment oceans biota -152.2917 -152.2917 57.7208 57.7208 2011-07-01 2013-07-06 In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number. NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Originator data format 0.192 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0123400.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://doi.org/10.7289/v59884zw HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/123400 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/123400 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0072/0123400/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0123400 v1.1 was published. 2015-01-13T14:24:07Z NCEI Accession 0123400 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0123400/1.1 HTTPS NCEI Accession 0123400 v1.1 published 2015-01-13T14:24:07Z download NCEI Accession 0123400 was revised and v2.2 was published. Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. 2021-10-07T21:33:12Z NCEI Accession 0123400 v2.2 publication https://www.ncei.noaa.gov/archive/accession/0123400/2.2 HTTPS NCEI Accession 0123400 v2.2 published 2021-10-07T21:33:12Z download dataset Parameter or Variable: Average pH that Tanner crab was held at for 602 days before their hemolymph was removed and anlayzed for immune response.; Abbreviation: Average pH; Controlled vocabulary name: None; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: calculated average; Analyzing instrument: a Durafet II or III pH probe; Detailed sampling and analyzing information: This is the average pH that each crab was exposed to for 602 days. The original pH, alkalinity, DIC values can be found in the data set by Lead PI Robert Foy entitled Effects of Ocean Acidification on Federally Managed Crab Species in Alaska; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL2-H fluorenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL3-H fluorenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL2-H fluorenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F HC FL2-H to calculate internal cell pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the halinocytes cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL3-H fluorenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method refer; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Calcualted semi-granular and granular cell internal pH; Abbreviation: Calculated SGC+GC pHi; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Calcualted halinocyte cell internal pH; Abbreviation: Calculated HC pHi; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: External hemolymph pH; Abbreviation: Hemolymph pHe; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Corning pH-30 electrode; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Total number of cells in the hemolymph that are semi-granular and granular hemocytes; Abbreviation: Total Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Total number of cells in the hemolymph that are halinocyte cells; Abbreviation: Total Cell HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Total number of dead cells in the hemolymph that are semi-granular and granular hemocytes; Abbreviation: Dead Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Total number of dead halinocyte cells in the hemolymph; Abbreviation: Dead Cell HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Percentage of dead cells for semi-granular and granular hemocytes in the hemolymph; Abbreviation: % Dead Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Percentage of dead halinocyte cells in the hemolymph; Abbreviation: % Dead HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration Parameter or Variable: Percentage of cellsthat have Phagocytosis properties; Abbreviation: % Phagocytosis; Observation type: experiment studied; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).; Detailed sampling and analyzing information: All hemocytes that contained 3 beads or more are considered to be highly phagocytic; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration repository NOAA National Centers for Environmental Information NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015. 2015-04-22T00:00:00 asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian Flow Cytometer Flow Cytometer A method to enumerate bacterial and phytoplankton cells in seawater and estimate their spherical diameter Flow cytometers are laboratory based instruments used to measure cells and particles from aqueous samples. The instrument was initially developed for analysis of Human blood cells for medical purposes, but this technology was adopted by the ocean science community by several investigators, principally Penny Chischolm out of the Massachusetts Institute of Technology, in late 20th century. Since then, the technique has been broadly applied, and it is a unique measurement of microbial populations in the ocean because it provides one at a time measures of cells, to extrapolate features such as fluorescence and cell size. Flow cytometers have been adapted for a number of oceanographic applications such as ship board measurement, and more precise measurement of planktonic fractions. Additionally, the flow cytometer has been adapted for in-situ deployment (see the corresponding nodc instrument code).