{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "accessRights": "public",
  "bureauCode": [
    "005:18"
  ],
  "contactPoint": {
    "fn": "Narrowe, Adrienne",
    "hasEmail": "mailto:adrienne.narrowe@usda.gov"
  },
  "description": "<p>A 24-h in vitro culturing method was used to determine whether fructooligosaccharide (FOS) treatment effects on the human gut microbiome has a differential effect by age, using fecal samples  from 18 adults in 3 age groups with 6 individuals for each group, young adult (25–35 years of age), adult (36–50 years of age), and older adult (51–70 years of age). Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883714: <a href=\"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714\">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714</a>), total bacterial qPCR, Bifidobacterium genus specific q-PCR, and short chain fatty acid concentrations. </p>\n<p>Resources in this dataset:</p>\n<ul>\n<li>\n<p>Resource Title: Sample metadata\nFile Name: FOS_sample_attributes.csv</p>\n</li>\n<li>\n<p>Resource Title: Short Chain Fatty Acid concentrations\nFile Name: FOS_SCFA_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Total bacterial and Bifidobacterium genus qPCR\nFile Name: total_bacteria_and_Bifidobacterium_specific_qPCR.csv</p>\n</li>\n</ul>",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/44540789",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "application/csv",
      "title": "FOS_sample_attributes_0.csv"
    },
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/44540792",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "text/plain",
      "title": "FOS_SCFA_data_0.csv"
    },
    {
      "@type": "dcat:Distribution",
      "downloadURL": "https://ndownloader.figshare.com/files/44540795",
      "format": "csv",
      "license": "https://creativecommons.org/licenses/by/4.0/",
      "mediaType": "application/csv",
      "title": "total_bacteria_and_Bifidobacterium_specific_qPCR_0.csv"
    }
  ],
  "identifier": "10.15482/USDA.ADC/1529151",
  "keyword": [
    "16S rRNA sequence data",
    "ARS",
    "NP306",
    "bifidobacteria",
    "data.gov",
    "fructooligosaccharides",
    "prebiotics",
    "short chain fatty acids"
  ],
  "license": "https://creativecommons.org/licenses/by/4.0/",
  "modified": "2025-11-21",
  "programCode": [
    "005:040"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "Agricultural Research Service"
  },
  "spatial": [
    {
      "@type": "Location",
      "prefLabel": "{\"type\": \"Point\", \"coordinates\": [-75.187092456102, 40.078126249741]}"
    }
  ],
  "temporal": [
    {
      "@type": "PeriodOfTime",
      "endDate": "2021-05-01",
      "startDate": "2021-05-01"
    }
  ],
  "title": "Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner"
}