gov.noaa.nodc:0304024 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-02-05T19:11:28Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) NCCOS Assessment: Investigating the effects of chronic turbidity exposure with elevated temperature to priority Florida coral species, Orbicella faveolata 2024-02-13 to 2024-05-07 (NCEI Accession 0304024) 2025-05-20 publication 2025-05-05 creation International DOI Foundation (IDF) 10.25921/kx8p-8m95 NOAA National Centers for Environmental Information gov.noaa.nodc:0304024 NCEI Archive Management System 0304024 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information resourceProvider Cheryl M. Woodley NOAA National Centers for Coastal Ocean Science (NCCOS) cheryl.woodley@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information principalInvestigator Cheryl M. Woodley NOAA National Centers for Coastal Ocean Science (NCCOS) cheryl.woodley@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information pointOfContact NOAA National Centers for Coastal Ocean Science (NCCOS) NCCOS Data Manager nccos.data@noaa.gov https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information pointOfContact Xaymara Serrano US DOC/NOAA/NMFS/Southeast Regional Office/Habitat Conservation Division/Atlantic Branch collaborator Jocelyn L. Karazsia US DOC/NOAA/NMFS/Southeast Regional Office/Habitat Conservation Division/Atlantic Branch Jocelyn.Karazsia@noaa.gov collaborator Mark C Ladd NOAA Southeast Fisheries Science Center (NMFS/SEFSC) https://www.fisheries.noaa.gov/about/southeast-fisheries-science-center HTTPS NOAA Southeast Fisheries Science Center (NMFS/SEFSC) website Institution web page information collaborator Jennifer Ness National Institute of Standards and Technology collaborator Miller, Carl V. CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Moffitt, Zachary J. CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Saldana, Courtney CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Pollock, Arielle CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author McDonald, E. Murphy CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Saunders, Janet E. CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) janet.saunders@noaa.gov https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Kothera, Ronald T. CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Radick, Ray CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author May, Lisa A. CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Helmericks, Samantha CSS, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author NOAA National Centers for Coastal Ocean Science (NCCOS) https://coastalscience.noaa.gov/ HTTPS NOAA National Centers for Coastal Ocean Science (NCCOS) website Institution web page information contributor NOAA Fisheries (NMFS) https://www.fisheries.noaa.gov/ HTTPS NOAA Fisheries (NMFS) website Institution web page information contributor tableDigital This dataset is comprised of multiple analyses from a 90-day laboratory exposure experiment to determine the chronic effects of fine-sediment generated turbidity in combination with increased temperature on the health of Orbicella faveolate coral nubbins. The biochemical analyses were performed throughout the duration of the experiment and biological endpoints were performed at the end of the 90-day turbidity and elevated temperature experiment. Photographs, buoyant weight and PAM fluorometry measurements were performed weekly, while tissue regeneration post-wounding, dry weight, total lipids, total proteins, and symbiont density were measured at the end of the 90-day exposure. Data are in spreadsheet and image format. The purpose of this work is to fill data gaps related to the effects of chronic turbidity exposure on Florida’s shallow-water stony corals. These new data are intended to be used in Florida’s efforts to improve water quality to ensure protection of Florida’s coral reefs. The data from the turbidity-elevated temperature study is intended to provide resource managers with information regarding possible additive or synergistic effects that can together lower safety thresholds for coral health. This provides actionable information for managing nearshore activities (dredging, construction, etc.) that create elevated turbid conditions for coral, particularly during periods of elevated temperatures. Funding Agency: Florida Department of Environmental Protection (FL DEP) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0304024 Preview graphic PNG 0304024 NCEI ACCESSION NUMBER 2025-04-16 publication CORAL images lipids turbidity theme NODC DATA TYPES THESAURUS camera fluorometer laboratory analysis microscope instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory analyses laboratory experiments physical theme NODC OBSERVATION TYPES THESAURUS NOAA Fisheries NOAA National Centers for Coastal Ocean Science dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA National Centers for Coastal Ocean Science dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Coral Reef Conservation Program (CRCP) CORAL REEF STUDIES project NODC PROJECT NAMES THESAURUS Coastal Waters of Florida North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication Florida Department of Environemntal Protection (DEP) Coral Protection and Restoration Program (CPRP) dataCentre Contributing Data Centers DOC/NOAA/NMFS > National Marine Fisheries Service, NOAA, U.S. Department of Commerce DOC/NOAA/NOS/NCCOS > National Centers for Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian 31314 Determining a Protective Turbidity Criterion for Coral project CRCP Project Genetic Data Sets > Proteomic Analysis Numeric Data Sets > Biology Numeric Data Sets > Water Quality Visual Images > Corals theme CoRIS Discovery Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Zooxanthellae EARTH SCIENCE > Biosphere > Zoology > Corals EARTH SCIENCE > Biosphere > Zoology > Corals > ESA Listed Species EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Damage Assessment > Coastal development EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Damage Assessment > Coral Recovery EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Proteomic Analysis EARTH SCIENCE > Biosphere > Zoology > Corals > Scleractinia (stony corals) EARTH SCIENCE > Terrestrial Hydrosphere > Water Quality/Water Chemistry > Turbidity theme CoRIS Theme Thesaurus EARTH SCIENCE > OCEANS > OCEAN OPTICS > TURBIDITY EARTH SCIENCE > SOLID EARTH > GEOMORPHIC LANDFORMS/PROCESSES > COASTAL LANDFORMS > CORAL REEFS theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NCCOS Research Data Type > Laboratory Experiment NCCOS Research Priority > Coastal Change: Vulnerability, Mitigation, and Restoration NCCOS Research Priority > Stressor Impacts and Mitigation NCCOS Research Topic > Vulnerability and Risk Assessment theme NCCOS Keywords Dinoflagellate Zoox Counts Maximum Quantum Yield Orbicella faveolata Buoyant Weight Orbicella faveolata Dry Weight Orbicella faveolata Tissue Regeneration Orbicella faveolata Total Lipids Orbicella faveolata Total Protein Orbicella faveolata Triglycerides Sediment Particle Size theme Provider Keywords CAMERA > CAMERA FLUOROMETERS > FLUOROMETERS MICROSCOPES > MICROSCOPES instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Analytical Balance ImageJ software Imaging PAM M-series chlorophyll fluorometer Malvern Mastersizer 3000 MeshLab software Olympus MVX10 research macro zoom (0.63x) microscope equipped with a DP71 digital camera Plate Reader SpectraMax instrument Provider Instruments COUNTRY/TERRITORY > United States of America > Florida > Dade County > Miami (25N080W0018) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Miami (25N080W0018) place CoRIS Place Thesaurus OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NCCOS Research Locations > Region > Atlantic Ocean NCCOS Research Locations > U.S. States and Territories > Florida place NCCOS Location Keywords Florida Coast Miami, FL Port Everglades, FL place Provider Place Names DJERNP Send2NCEI Submission Package ID accessLevel: public otherRestrictions Cite as: Miller, Carl V.; Moffitt, Zachary J.; Saldana, Courtney; Pollock, Arielle; McDonald, E. Murphy; Saunders, Janet E.; Kothera, Ronald T.; Radick, Ray; May, Lisa A.; Helmericks, Samantha (2025). NCCOS Assessment: Investigating the effects of chronic turbidity exposure with elevated temperature to priority Florida coral species, Orbicella faveolata 2024-02-13 to 2024-05-07 (NCEI Accession 0304024). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/kx8p-8m95. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license This dataset has been dedicated to the public domain under a Creative Commons CC0 1.0 Universal (CC0 1.0) Public Domain Dedication. SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) Anschutz, P., & Charbonnier, C. (2020). Sampling pore water at a centimeter resolution in sandy permeable sediments of lakes, streams, and coastal zones. Limnology and Oceanography: Methods, 19(2), 96–114. https://doi.org/10.1002/lom3.10408 2020-12-20 publication International DOI Foundation (IDF) https://doi.org/10.1002/lom3.10408 https://doi.org/10.1002/lom3.10408 HTTPS https://doi.org/10.1002/lom3.10408 related journal article information Anschutz, Pierre University of Bordeaux, CNRS, EPOC, EPHE, UMR 5805 Pessac France author Charbonnier, Céline University of Bordeaux, CNRS, EPOC, EPHE, UMR 5805 Pessac France author Wiley publisher documentDigital Limnology & Ocean Methods 19(2) ISSN 1541-5856, ISSN 1541-5856, pp. 96-114 crossReference sciencePaper Bialek, E. L. (1966). Handbook of Oceanographic Tables. U.S. Naval Oceanographic Office, Washington, D.C. Available from: https://apps.dtic.mil/sti/pdfs/AD0655638.pdf 1966 publication https://apps.dtic.mil/sti/pdfs/AD0655638.pdf HTTPS https://apps.dtic.mil/sti/pdfs/AD0655638.pdf related journal article information Bialek, Eugene L. U.S. Naval Oceanographic Office author U.S. Naval Oceanographic Office publisher documentDigital crossReference sciencePaper Coman, C., Solari, F. A., Hentschel, A., Sickmann, A., Zahedi, R. P., & Ahrends, R. (2016). Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX): A Combinatorial Multimolecular Omics Approach for Systems Biology. Molecular & Cellular Proteomics, 15(4), 1435–1466. https://doi.org/10.1074/mcp.m115.053702 2016-04 publication International DOI Foundation (IDF) https://doi.org/10.1074/mcp.m115.053702 https://doi.org/10.1074/mcp.m115.053702 HTTPS https://doi.org/10.1074/mcp.m115.053702 related journal article information Coman, Cristina author Solari, Fiorella Andrea author Hentschel, Andreas author Sickmann, Albert author Zahedi, René Peiman author Ahrends, Robert author Elsevier BV publisher documentDigital Molecular & Cellular Proteomics 15(4) ISSN 1535-9476, pp. 1435-1466 crossReference sciencePaper Dodge, R. E., Wyers, S. C., Frith, H. R., Knap, A. H., Smith, S. R., Cook, C. B., & Sleeter, T. D. (1984). Coral calcification rates by the buoyant weight technique: Effects of alizarin staining. Journal of Experimental Marine Biology and Ecology, 75(3), 217–232. https://doi.org/10.1016/0022-0981(84)90167-9 1984-03 publication International DOI Foundation (IDF) https://doi.org/10.1016/0022-0981(84)90167-9 https://doi.org/10.1016/0022-0981(84)90167-9 HTTPS https://doi.org/10.1016/0022-0981(84)90167-9 related journal article information Dodge, Richard E. author Wyers, Sheila C. author Frith, H.R. author Knap, Anthony H. author Smith, S.R. author Cook, C.B. author Sleeter, T.D. author Elsevier BV publisher documentDigital Journal of Experimental Marine Biology and Ecology 75(3) ISSN 0022-0981, pp. 217-232 crossReference sciencePaper Ghosh, S., Gepstein, S., Heikkila, J. J., & Dumbroff, E. B. (1988). Use of a scanning densitometer or an ELISA plate reader for measurement of nanogram amounts of protein in crude extracts from biological tissues. Analytical Biochemistry, 169(2), 227–233. https://doi.org/10.1016/0003-2697(88)90278-3 1988-03 publication International DOI Foundation (IDF) https://doi.org/10.1016/0003-2697(88)90278-3 https://doi.org/10.1016/0003-2697(88)90278-3 HTTPS https://doi.org/10.1016/0003-2697(88)90278-3 related journal article information Ghosh, Sibdas author Gepstein, Shimon author Heikkila, John J. author Dumbroff, Erwin B. author Elsevier BV publisher documentDigital Analytical Biochemistry 169(2) ISSN 0003-2697, pp. 227-233 crossReference sciencePaper Gorin, D. R., Cordts, P. R., LaMorte, W. W., & Menzoian, J. O. (1996). The influence of wound geometry on the measurement of wound healing rates in clinical trials. Journal of Vascular Surgery, 23(3), 524–528. https://doi.org/10.1016/s0741-5214(96)80021-8 1996-03 publication International DOI Foundation (IDF) https://doi.org/10.1016/s0741-5214(96)80021-8 https://doi.org/10.1016/s0741-5214(96)80021-8 HTTPS https://doi.org/10.1016/s0741-5214(96)80021-8 related journal article information Gorin, Daniel R. author Cordts, Paul R. author LaMorte, Wayne W. author Menzoian, James O. author Elsevier BV publisher documentDigital Journal of Vascular Surgery 23(3) ISSN 0741-5214, pp. 524-528 crossReference sciencePaper Jokiel P.L., Maragos J.E., Franzisket L. (1978) Coral growth: buoyant weight technique. In: Stoddart DR, Johannes RE, editors. Coral Reefs: Research Methods. Paris: UNESCO. 529–541 1978 publication https://unesdoc.unesco.org/ark:/48223/pf0000029306.locale=en HTTPS https://unesdoc.unesco.org/ark:/48223/pf0000029306.locale=en related journal article information Jokiel P.L. Hawaiʻi Institute of Marine Biology author Maragos J.E. U.S. Army Corps of Engineers, Pacific Ocean Division author Franzisket L. Westfalisches Landesmuseum fur Naturkunde author United Nations Educational, Scientific and Cultural Organization publisher documentDigital Progress in Physical Geography: Earth and Environment 41(5) ISBN 92-3-101491-9, pp. 529-541 crossReference sciencePaper Kitchener, B. G., Wainwright, J., & Parsons, A. J. (2017). A review of the principles of turbidity measurement. Progress in Physical Geography: Earth and Environment, 41(5), 620–642. https://doi.org/10.1177/0309133317726540 2017-08-23 publication International DOI Foundation (IDF) https://doi.org/10.1177/0309133317726540 https://doi.org/10.1177/0309133317726540 HTTPS https://doi.org/10.1177/0309133317726540 related journal article information Kitchener, Ben GB University of Sheffield, Sheffield, UK author Wainwright, John Durham University, Durham, UK author Parsons, Anthony J University of Sheffield, Sheffield, UK author SAGE Publications publisher documentDigital Progress in Physical Geography: Earth and Environment 41(5) ISSN 0309-1333, ISSN 1477-0296, pp. 620-642 crossReference sciencePaper May, L. A., Burnett, A. R., Miller, C. V., Pisarski, E., Webster, L. F., Moffitt, Z. J., Pennington, P., Wirth, E., Baker, G., Ricker, R., & Woodley, C. M. (2020). Effect of Louisiana sweet crude oil on a Pacific coral, Pocillopora damicornis. Aquatic Toxicology, 222, 105454. https://doi.org/10.1016/j.aquatox.2020.105454 2020-05 publication International DOI Foundation (IDF) https://doi.org/10.1016/j.aquatox.2020.105454 https://doi.org/10.1016/j.aquatox.2020.105454 HTTPS https://doi.org/10.1016/j.aquatox.2020.105454 related journal article information May, Lisa A. author Burnett, Athena R. author Miller, Carl V. author Pisarski, Emily author Webster, Laura F. author Moffitt, Zachary J. author Pennington, Paul author Wirth, Edward author Baker, Greg author Ricker, Robert author Woodley, Cheryl M. author Elsevier BV publisher documentDigital Aquatic Toxicology 222 ISSN 0166-445X, p. 105454 crossReference sciencePaper May, L. A., Miller, C. V., Moffitt, Z. J., Balthis, L., Karazsia, J., Wilber, P., & Woodley, C. M. (2023). Acute turbidity exposures with Port of Miami sediments impact Orbicella faveolata tissue regeneration. Marine Pollution Bulletin, 193, 115217. https://doi.org/10.1016/j.marpolbul.2023.115217 2023-08 publication International DOI Foundation (IDF) https://doi.org/10.1016/j.marpolbul.2023.115217 https://doi.org/10.1016/j.marpolbul.2023.115217 HTTPS https://doi.org/10.1016/j.marpolbul.2023.115217 related journal article information May, Lisa A. author Miller, Carl V. author Moffitt, Zachary J. author Balthis, Len author Karazsia, Jocelyn author Wilber, Pace author Woodley, Cheryl M. author Elsevier BV publisher documentDigital Marine Pollution Bulletin 193 ISSN 0025-326X, p. 115217 crossReference sciencePaper C Million, W., & Kenkel, C. (2020). Phenotyping 3D coral models in MeshLab v1. https://doi.org/10.17504/protocols.io.bgbpjsmn 2020-05-13 publication International DOI Foundation (IDF) https://doi.org/10.17504/protocols.io.bgbpjsmn https://doi.org/10.17504/protocols.io.bgbpjsmn HTTPS https://doi.org/10.17504/protocols.io.bgbpjsmn related resource information C Million, Wyatt University of Technology Sydney author Kenkel, Carly author ZappyLab, Inc. publisher documentDigital crossReference sciencePaper Ralph, P. J., Schreiber, U., Gademann, R., Kühl, M., & Larkum, A. W. D. (2005). CORAL PHOTOBIOLOGY STUDIED WITH A NEW IMAGING PULSE AMPLITUDE MODULATED FLUOROMETER1. Journal of Phycology, 41(2), 335–342. https://doi.org/10.1111/j.1529-8817.2005.04034.x 2005-03-08 publication International DOI Foundation (IDF) https://doi.org/10.1111/j.1529-8817.2005.04034.x https://doi.org/10.1111/j.1529-8817.2005.04034.x HTTPS https://doi.org/10.1111/j.1529-8817.2005.04034.x related journal article information Ralph, Peter J. author Schreiber, Ulrich author Gademann, Rolf author Kühl, Michael author Larkum, Anthony W. D. author Wiley publisher documentDigital Journal of Phycology 41(2) ISSN 0022-3646, ISSN 1529-8817, pp. 335-342 crossReference sciencePaper Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671–675. https://doi.org/10.1038/nmeth.2089 2012-06-28 publication International DOI Foundation (IDF) https://doi.org/10.1038/nmeth.2089 https://doi.org/10.1038/nmeth.2089 HTTPS https://doi.org/10.1038/nmeth.2089 related journal article information Schneider, Caroline A author Rasband, Wayne S author Eliceiri, Kevin W author Springer Science and Business Media LLC publisher documentDigital Nat Methods 9(7) ISSN 1548-7091, ISSN 1548-7105, pp. 671-675 crossReference sciencePaper Zamoum, T., & Furla, P. (2012). Symbiodinium isolation by NaOH treatment. Journal of Experimental Biology. https://doi.org/10.1242/jeb.074955 2012-01-01 publication International DOI Foundation (IDF) https://doi.org/10.1242/jeb.074955 https://doi.org/10.1242/jeb.074955 HTTPS https://doi.org/10.1242/jeb.074955 related journal article information Zamoum, Thamilla Université Nice Sophia Antipolis author Furla, Paola Université Nice Sophia Antipolis author The Company of Biologists publisher documentDigital Journal of Experimental Biology ISSN 1477-9145, ISSN 0022-0949 crossReference sciencePaper Miller, Carl V.; Moffitt, Zachary J.; Saldana, Courtney; Pollock, Arielle; McDonald, E. Murphy; Saunders, Janet E.; Kothera, Ronald T.; Radick, Ray; May, Lisa A.; Helmericks, Samantha (2025). NCCOS Assessment: Investigating the effects of chronic turbidity exposure to priority Florida coral species, Orbicella faveolata 2023-09-19 to 2023-12-12 (NCEI Accession 0303582). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ctar-yv86. 2025-04-29 publication International DOI Foundation (IDF) https://doi.org/10.25921/ctar-yv86 NOAA National Centers for Environmental Information gov.noaa.nodc:0303582 https://doi.org/10.25921/ctar-yv86 HTTPS https://doi.org/10.25921/ctar-yv86 Related NCEI Data Accession information Miller, Carl V. Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Moffitt, Zachary J. Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Saldana, Courtney Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Pollock, Arielle Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author McDonald, E. Murphy Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Saunders, Janet E. Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) janet.saunders@noaa.gov https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Kothera, Ronald T. Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Radick, Ray Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author May, Lisa A. Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author Helmericks, Samantha Consolidated Safety Services-Dynamac, under contract to NOAA National Centers for Coastal Ocean Science (NCCOS) https://www.css-inc.com/ HTTPS Consolidated Safety Services-Dynamac (CSS) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference collection eng; USA utf8 environment oceans biota -80.1818346 -80.1449857 25.766066 25.781201 2024-02-13 2024-05-07 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Excel PDF PNG 35.952 https://doi.org/10.25921/kx8p-8m95 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/304024 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/304024 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0236/0304024/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0304024 v1.1 was published. 2025-05-20T20:57:50Z NCEI Accession 0304024 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0304024/1.1 HTTPS NCEI Accession 0304024 v1.1 published 2025-05-20T20:57:50Z download nonGeographicDataset Parameter or Variable: Maximum Quantum Yield (calculated); Units: Fv/Fm; Observation Category: laboratory analysis; Sampling Instrument: Imaging PAM M-series chlorophyll fluorometer; Sampling and Analyzing Method: Pulse amplitude modulated (PAM) measurements will be collected on treatment groups as previously described (May et al., 2020). Briefly, sediment is removed from corals as described above and fragments are placed in a custom Teflon stand in ASW in a clean glass crystallizing dish, 1-2 h before the end of the dark cycle. Lighting is provided by green headlamps to minimize any chlorophyll fluorescence excitation effects. Data are collected on the top of the OFAV fragments. During data analysis, a single circular area of interest is selected near the center of each fragment image prior to tissue wounding. After wounding, the area of interest for the top of the fragments is placed adjacent to, but not overlapping, the wound. Replicate fragments are evaluated as previously reported (Ralph et al., 2005).; Data Quality Method: Significant differences in Maximum Quantum Yield were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met. A mixed-model was used to test for effects over time. Parameter or Variable: Dinoflagellate Zoox Counts (calculated); Units: Dinoflagellates/mm2; Observation Category: laboratory analysis; Sampling Instrument: MeshLab software; Sampling and Analyzing Method: A sub-fragment will be removed at the termination of the experiment and symbionts extracted using a sodium hydroxide solution adapted from Zamoum and Furla (2012). The surface area of the coral fragment will be determined using photogrammetry and MeshLab software according to the method of Million and Kenkel (2020).; Data Quality Method: Significant differences in Zoox Counts were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Orbicella faveolata Tissue Regeneration (calculated); Units: Percent Regeneration; Observation Category: laboratory analysis; Sampling Instrument: Olympus MVX10 research macro zoom (0.63x) microscope equipped with a DP71 digital camera; ImageJ software; Sampling and Analyzing Method: Wounding of fragments will occur 21 days prior to the 90 day experiment termination and photodocumented daily. Image analysis will be used to determine the percent healing and healing rate of each fragment. Wound healing measurements are conducted using a metric ruler level with the wound and is included in each photograph to normalize any shifts in distance that may affect the magnification of the wound. The imaging software translates the distance between mm marks on the ruler into pixels to conduct the image analysis and measurements of changes in the area and perimeter of the wound. Skeletal area for each coral image is determined using ImageJ (Schneider et al., 2012) with a modified macro from the NOAA/NCCOS Coral Disease and Health Consortium website (https://cdhc.noaa.gov/education/coral_assay.aspx; macro is provided as Appendix III). Total area of bare skeleton and wound perimeter values are recorded for each fragment at time 0 and each experimental time point.; Data Quality Method: Significant differences in Tissue Regeneration were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met. A mixed-model was used to test for effects over time. Parameter or Variable: Orbicella faveolata Dry Weight (calculated); Units: grams; Observation Category: laboratory analysis; Sampling Instrument: Analytical Balance; Sampling and Analyzing Method: Buoyant (wet) weight is converted to dry weight based on seawater density derived from knowing the temperature, salinity and using aragonite density of 2.93 g cm-3.; Data Quality Method: Significant differences in Dry Weight over the course of the experiment were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met. A mixed-model was used to test for effects over time. Significant differences in Final Gain in Dry Weight were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Orbicella faveolata Buoyant Weight (measured); Units: grams; Observation Category: laboratory analysis; Sampling Instrument: Analytical Balance; Sampling and Analyzing Method: Net growth of coral fragments is determined based on the buoyant weight technique of Jokiel et al. (1978). Each coral fragment is buoyantly weighed every two weeks using an analytical balance equipped with an underhook.; Data Quality Method: Significant differences in Buoyant Weight over the course of the experiment were determined using repeated measures ANOVA followed by Tukey’s posttest if parameters were met and a nonparametric ANOVA with rank transformed data followed by pairwise comparisons using paired t-tests if they were not met. A mixed-model was used to test for effects over time. Significant differences in Final Gain in Buoyant Weight were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Orbicella faveolata Total Protein (measured); Units: grams; Observation Category: laboratory analysis; Sampling Instrument: Plate Reader; Sampling and Analyzing Method: Total soluble proteins were isolated using a modified Comen’s method. Proteins were quantified using a Pierce bicinchoninic acid assay on a Plate Reader; Data Quality Method: Significant differences in Total Proteins were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Orbicella faveolata Total Lipids (measured); Units: grams; Observation Category: laboratory analysis; Sampling Instrument: Analytical Balance; Sampling and Analyzing Method: Total lipids were isolated using a modified Comen’s method. Post-isolation, lipids were dried in a pre-weighed vial and the mass of the dried lipids was calculated by subtracting the initial weight of the vial from the final weight of the vial containing the dried lipids.; Data Quality Method: Significant differences in Total Lipids were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Orbicella faveolata Triglycerides (measured); Units: micromoles; Observation Category: laboratory analysis; Sampling Instrument: SpectraMax; Sampling and Analyzing Method: 100 mg of coral powder was resuspended in 4.5 mL of anhydrous ethanol and centrifuged. The supernatant was removed and triglycerides were measured using a triglyceride colorimetric assay kit.; Data Quality Method: Significant differences in Total Lipids were determined using one-way ANOVA followed by Tukey’s posttest if parametric assumptions were met and a nonparametric Kruskal-Wallis test followed by a Dunn’s posttest if assumptions were not met. Parameter or Variable: Sediment Particle Size (measured); Units: micrograms; Observation Category: laboratory analysis; Sampling Instrument: Malvern Mastersizer 3000; Sampling and Analyzing Method: Turbidity water samples of each treatment were collected, centrifuged, and dried down. The samples were run on a Malvern Mastersizer 3000 to determine particle size distribution (Dv) of each treatment sample.; Data Quality Method: Significant differences in Particle Size were determined using a nonparametric (Kruskal-Wallis) test with Dunn’s posttest. asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian camera camera An instrument for recording images. fluorometer fluorometer fluorometer laboratory analysis laboratory analysis use as an observation type microscope microscope instrument is used in lab analyses A microscope is an instrument used to see objects that are too small for the naked eye.