{
  "@type": "dcat:Dataset",
  "accessLevel": "public",
  "bureauCode": [
    "009:25"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "NIH/NCBI Data Team",
    "hasEmail": "mailto:info@ncbi.nlm.nih.gov"
  },
  "description": "The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "description": "Access the complete Patent AT-E401415-T1: [Translated] DIRECT RT-PCR ON MICROTITER PLATES WITH IMMOBILIZED OLIGONUCLEOTIDE on the official website.",
      "downloadURL": "https://pubchem.ncbi.nlm.nih.gov/patent/AT-E401415-T1",
      "mediaType": "text/html",
      "title": "Official Data Source"
    }
  ],
  "identifier": "https://healthdata.gov/api/views/dyqc-rdew",
  "issued": "2025-09-05",
  "keyword": [
    "chemistry",
    "innovation",
    "patent",
    "pubchem",
    "research"
  ],
  "landingPage": "https://healthdata.gov/d/dyqc-rdew",
  "modified": "2025-09-06",
  "programCode": [
    "009:066"
  ],
  "publisher": {
    "@type": "org:Organization",
    "name": "National Center for Biotechnology Information (NCBI)"
  },
  "theme": [
    "NIH"
  ],
  "title": "Patent AT-E401415-T1: [Translated] DIRECT RT-PCR ON MICROTITER PLATES WITH IMMOBILIZED OLIGONUCLEOTIDE"
}