{
  "accessLevel": "public",
  "bureauCode": [
    "010:12"
  ],
  "contactPoint": {
    "@type": "vcard:Contact",
    "fn": "Katy E Klymus",
    "hasEmail": "mailto:kklymus@usgs.gov"
  },
  "description": "The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.",
  "distribution": [
    {
      "@type": "dcat:Distribution",
      "accessURL": "https://doi.org/10.5066/P9BIGOS5",
      "description": "Landing page for access to the data",
      "format": "XML",
      "mediaType": "application/http",
      "title": "Digital Data"
    },
    {
      "@type": "dcat:Distribution",
      "description": "The metadata original format",
      "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5f85c8b682cebef40f14c5d0.xml",
      "format": "XML",
      "mediaType": "text/xml",
      "title": "Original Metadata"
    }
  ],
  "identifier": "http://datainventory.doi.gov/id/dataset/USGS_5f85c8b682cebef40f14c5d0",
  "keyword": [
    "CERC molecular lab",
    "Clinch River",
    "Tennesee",
    "USGS:5f85c8b682cebef40f14c5d0",
    "Wallen's Bend",
    "assay optimization",
    "biota",
    "eDNA",
    "freshwater mussels",
    "qPCR"
  ],
  "modified": "2020-11-19T00:00:00Z",
  "publisher": {
    "@type": "org:Organization",
    "name": "U.S. Geological Survey"
  },
  "spatial": "-83.0126, 36.579282, -83.004042, 36.583719",
  "theme": [
    "geospatial"
  ],
  "title": "Mucket eDNA detection in Wallen’s Bend, Clinch River, Tennessee, September 2019"
}