{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Katy E Klymus", "hasEmail": "mailto:kklymus@usgs.gov"}, "description": "This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina  and Cumberlandia monodonta from water samples (eDNA).", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P16VAVZF", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.66d1cb43d34ebebd6af05eb4.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_66d1cb43d34ebebd6af05eb4", "keyword": ["Boone County, Missouri", "Columbia Environmental Research Center", "USGS:66d1cb43d34ebebd6af05eb4", "biota", "environmental DNA", "mollusks", "polymerase chain reaction"], "modified": "2024-09-04T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-92.284999, 38.910784, -92.273644, 38.912612", "theme": ["geospatial"], "title": "Development and optimization of  species-specific qPCR assays for eDNA detection of the mucket (Ortamannia ligamentina) and spectaclecase (Cumberlandia monodonta) mussels\u2019 male mitotype"}