{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Craig Jackson", "hasEmail": "mailto:cjackson@usgs.gov"}, "description": "Detection of environmental DNA (eDNA) has become a commonly used surveillance\nmethod for threatened or invasive vertebrates in both aquatic and terrestrial\nenvironments. However, use of eDNA methodologies for the detection of aquatic\ninvertebrates (e.g., crayfish and insects) has been limited. Environmental DNA protocols\ncan be especially useful for endangered invertebrates such as the Hine\u2019s emerald\ndragonfly (Somatochlora hineana) where conservation efforts have been greatly\nhindered by the training, time, overall costs, and environmental impacts associated with\nconducting surveys in the calcareous fens occupied by this species. An essential step in\ndeveloping such a protocol is to evaluate the dynamics of eDNA concentration under\ncontrolled and field conditions. In this study we examined the persistence and\naccumulation of eDNA from captive S. hineana larvae in experimental mesocosms at\ntemperatures (5.0\u00b0C and 16.0\u00b0C) that reflect seasonal variation in their natural habitat,\nand we evaluated the usefulness of eDNA protocols for studying the distribution and\nabundance of invertebrates by assessing patterns of eDNA distribution for the Hine\u2019s\nemerald dragonfly and its symbiont the devil crayfish, (Cambarus [=Lacunicambarus]\ndiogenes) in the field over several months. In mesocosms, S. hineana eDNA persisted\nlonger at 5.0\u00b0C but accumulated more readily at 16.0\u00b0C. In the field, life-history events\naffected seasonal variations in eDNA more significantly and consistently than\ntemperature for both species. These data can be used to aid in conservation efforts for\nS. hineana and similar aquatic invertebrates.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P1IK9KAG", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.662ba990d34ea70bd5f11c6f.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_662ba990d34ea70bd5f11c6f", "keyword": ["Door County, Wisconsin", "HED", "Hine's Emerald Dragonfly", "USGS:662ba990d34ea70bd5f11c6f", "University of South Dakota-Vermillion", "biota", "eDNA", "endangered", "qPCR"], "modified": "2024-06-06T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-97.6465, 42.4235, -87.0996, 45.0270", "theme": ["geospatial"], "title": "Data Release for Using Environmental DNA to Effectively Detect Aquatic Arthropods: Monitoring Seasonal Changes in eDNA Concentration"}