{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Alkharouf, Nadim W.", "hasEmail": "mailto:nalkharouf@towson.edu"}, "description": "<p>NOTE: This dataset is no longer publicly available.</p>\n<p>BBGD (<a href=\"http://bioinformatics.towson.edu/BBGD/\">http://bioinformatics.towson.edu/BBGD/</a>) was developed as a database for blueberry genomics. BBGD is both a sequence and gene expression database. It stores both EST and microarray data and allows scientists to correlate expression profiles with gene function. BBGD is a public online database. \"Presently, the main focus of the database is the identification of genes in blueberry that are significantly induced or suppressed after low temperature exposure. \"</p>\n<p>To gain a better understanding of changes in gene expression associated with cold acclimation in blueberry, the Rowland laboratory (USDA-ARS, Beltsville, MD)  has undertaken a genomics approach based on the analysis of Expressed Sequence Tags (ESTs). Initially, two standard cDNA libraries were constructed using RNA from cold-acclimated and non-acclimated floral buds of the blueberry cultivar \u2018Bluecrop\u2019 (<em>Vaccinium corymbosum</em> L.) and about 1200 5\u2019-end ESTs were generated from each of the libraries. About 100 3\u2019-end ESTs were generated from the cold-acclimated library as well.</p>\n<p>The Blueberry EST database contains EST sequences from a number blueberry libraries including cold acclimated and non-acclimated libraries. It also includes forward and reverse subtractive libraries.</p>\n<p>You can query the sequence database by clone ID, accession number or gene (clone) name below. Or you can get a list (in tabular) format of all the clones in a particular library by clicking on the library name on the left side navigation bar.</p>\n<p>Attribution for photo: <br><a href=\"http://www.ars.usda.gov/is/graphics/photos/sep12/d2601-1.htm\">D2601-1 -  Blueberry plant:</a> Copyright free, public domain photo by Mark Ehlenfeldt</p>", "distribution": [], "identifier": "10.15482/USDA.ADC/1173241", "keyword": ["ARS", "BBGD", "Cyanococcus", "NP301", "NP303", "Vaccinium", "blueberry", "data.gov", "database"], "license": "https://www.usa.gov/publicdomain/label/1.0/", "modified": "2024-02-08", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "title": "BBGD: an Online Database for Blueberry Genomic Data"}