{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          Various microbial, inflammatory and immune signals regulate the activation of dendritic cells (DC), determining their ability to interact with na\u00efve T cells and to produce cytokines that direct T cell development. In particular, CD40L and IL-1 cooperatively activate DC to secrete high levels of IL-12. The immuno-stimulatory capacity of such DC is otherwise not well-defined prompting further characterization of the effects of IL-1 and family members on DC activation in comparison with other pro-inflammatory stimuli.\n        \n        \n          Results\n          Human DC co-activated in vitro by CD40L and IL-1\u03b2 expressed numerous cytokine genes including IL-12\u03b2, IL-23 p19, IL-1\u03b2, IL-1\u03b1, IL-1Ra, IL-10, IL-6, IL-18 and IFN-\u03b3. These DC produced high levels of IL-12 protein and appeared capable of producing IFN-\u03b3. Potent CD4+ and CD8+ T cell-stimulatory properties were acquired by DC under conditions that also induced IL-12. Notably, these DC induced rapid differentiation of fluMP-specific CD8+ T cells. Molecules related to IL-1\u03b2, like IL-1\u03b1, co-induced IL-12 secretion whereas IL-18 did not. Conversely, the inhibitor IL-1Ra, produced endogenously by DC curtailed IL-12 production in response to CD40L.\n        \n        \n          Conclusions\n          IL-1 and IL-1Ra play a biologically-relevant role in the positive and negative regulation of DC activation. In conjunction with CD40L, IL-1 sends a powerful activation signal to DC that could be distinguished from other modes of activation. This signal enables the production of pro-inflammatory cytokines by DC, and enhances the differentiation of na\u00efve T cells into effectors of type-1 cellular immune responses.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC134468/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/nq7w-2633", "issued": "2025-07-14", "keyword": ["dendritic-cells", "il-12-production", "nih", "pro-inflammatory-cytokines", "t-cell-activation"], "landingPage": "https://healthdata.gov/d/nq7w-2633", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand"}