{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          MAdCAM-1 is an adhesion molecule expressed in Peyer's patches and lymphoid tissues which is mobilized by cytokines like TNF-\u03b1 and is a major determinant of lymphocyte trafficking to the gut in human inflammatory bowel disease (IBD). It has been suggested that both reactive oxygen and nitrogen metabolites participate in regulating adhesion molecule expression in response to TNF-\u03b1.\n        \n        \n          Methods\n          To examine how exogenous and endogenous sources of NO modulate MAdCAM-1 induction by TNF-\u03b1, we pre-treated mouse lymphatic endothelial cells with either long or short acting NO donors prior to TNF-\u03b1-stimulation, and measured MAdCAM-1 induction at 24 h.\n        \n        \n          Results and Discussion\n          DETA-NO, a long-acting NO donor, and SperNO, a rapid releasing NO donor both inhibited TNF-\u03b1-stimulated MAdCAM-1 expression in a concentration dependent manner. Both NO donors also reduced a4b7-dependent lymphocyte endothelial adhesion. Inhibition of endogenous NO production by either L-NAME, a non-selective NOS inhibitor, or by 1400 w, a selective iNOS inhibitor failed to induce, or potentiate TNF-\u03b1 regulated MAdCAM-1 expression.\n        \n        \n          Conclusions\n          Exogenous NO donors may be beneficial in the treatment of IBD, while endogenous nitric oxide synthases may be less effective in controlling adhesion molecule expression in response to cytokines.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC35355/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/qi95-y364", "issued": "2025-07-14", "keyword": ["inflammatory-bowel-disease", "madcam-1-expression", "nih", "nitric-oxide", "tnf-alpha"], "landingPage": "https://healthdata.gov/d/qi95-y364", "modified": "2025-09-06", "programCode": ["009:048"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "TNF-\u03b1 induced endothelial MAdCAM-1 expression is regulated by exogenous, not endogenous nitric oxide"}