gov.noaa.nodc:0306213 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-07-27T13:21:45Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae from laboratory experiment studies from 2019-03-11 to 2019-04-23 (NCEI Accession 0306213) 2025-07-16 publication 2025-07-15 creation International DOI Foundation (IDF) 10.25921/8kgb-wb45 NOAA National Centers for Environmental Information gov.noaa.nodc:0306213 NCEI Archive Management System 0306213 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Mara L. Spencer NOAA Alaska Fisheries Science Center (NMFS/AFSC) Mara.Spencer@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider Louise Copeman Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) Louise.Copeman@noaa.gov https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information principalInvestigator Thomas Hurst NOAA Alaska Fisheries Science Center (NMFS/AFSC) Thomas.Hurst@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Michelle Stowell Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information principalInvestigator Jessica Andrade Lynker Technologies principalInvestigator Copeman, Louise A. Cooperative Institute for Marine Ecosystem and Resource Studies, under grant from NOAA Alaska Fisheries Science Center https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Stowell, Michelle A. Cooperative Institute for Marine Ecosystem and Resource Studies https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Andrade, Jessica F. Lynker Technologies author Hurst, Thomas P. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information contributor Oregon State University (OSU) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information contributor tableDigital This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification and altered prey fatty acids on the early development of northern rock sole, Lepidopsetta polyxystra. Ocean acidification (OA) is predicted to affect the physiological rates of larval fish and invertebrates and is also expected to significantly impact marine fisheries through alteration of food webs. We examined whether mortality rates, body size, and condition of first-feeding larval northern rock sole were synergistically influenced by prey quality (essential fatty acids, EFAs) and high carbon dioxide (CO2) exposure. Larvae were exposed to ambient and high CO2 levels (~ 330 vs. 1020 µatm) and were fed diets with balanced or unbalanced EFA ratios for 7 weeks immediately following hatch. After 2 weeks, significant effects from CO2 and diet were observed, with the largest larvae occurring in the high CO2 exposure group that received a balanced EFA diet. After 5 weeks of exposure, the effects of elevated CO2 had a diminished impact on larval size, but larvae reared on the balanced EFA diet maintained higher lipid-based condition metrics than those fed an unbalanced EFA diet. Survival was variable across replicate tanks and not significantly different between treatments. This study suggests that L. polyxystra larvae are most vulnerable to OA and food web change at different points in their development. Further understanding of these ecosystem effects will be required to predict the impacts of OA on northern rock sole fisheries. To determine the effects of ocean acidification and altered prey fatty acids on the early development of northern rock sole larvae. Funding Information: NOAA's Ocean Acidification Program (Effects of OA on Alaskan groundfishes: identifying patterns and mechanisms of sensitivity and resiliency in physiological and behavioral performance, NRDD #17927) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0306213 Preview graphic PNG 0306213 NCEI ACCESSION NUMBER 2025-07-14 publication ARAGONITE SATURATION STATE DISSOLVED INORGANIC CARBON (DIC) DISSOLVED OXYGEN lipids partial pressure of carbon dioxide - water pH SALINITY SPECIES IDENTIFICATION - AGE SPECIES IDENTIFICATION - DRY WEIGHT total alkalinity WATER TEMPERATURE theme NODC DATA TYPES THESAURUS carbon dioxide (CO2) gas analyzer chromatograph oxygen sensor pH sensor temperature sensor instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory experiments physical survey - biological theme NODC OBSERVATION TYPES THESAURUS NOAA Alaska Fisheries Science Center Oregon State University dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Alaska Fisheries Science Center dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS North Pacific Ocean West Coast - US/Canada place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords NOAA Ocean Acidification Program (OAP) project Research Projects EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBONATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type 14_0 16_0 16_1w7 18_0 18_1w7 18_1w9 18_2w6 18_3w3 20_1w9 20_2w6 20_4w6 20_5w3 22_5w3 22_5w6 22_6w3 Age_weeks Bottle_ID CO2 CO2_trt DIC DO DPH DWT_FFA DWT_PL DWT_ST DWT_TAG DWT_Total DWT_sd Date Diet Diet_enrichment Diets_Type Experiment Life_stage Lipid Sample ID Perc_FFA Perc_PL Perc_ST Perc_TAG SL_sd Salinity Sample ID Sample Type TA TAG_ST Temp Time Weeks calcCO2 calcO Ar calcpH count h2oDQ mean_DWT mean_SL per_larvae_FFA per_larvae_PL per_larvae_ST per_larvae_TAG per_larvae_Total tank theme Provider Variable Abbreviations CO2 ANALYZERS > CO2 ANALYZERS GAS CHROMATOGRAPHS > GAS CHROMATOGRAPHS OXYGEN METERS > OXYGEN METERS PH METERS > PH METERS TEMPERATURE SENSORS > TEMPERATURE SENSORS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NOAA AFSC Laboratory Newport Oregon place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Copeman, Louise A.; Stowell, Michelle A.; Andrade, Jessica F.; Hurst, Thomas P. (2025). Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae from laboratory experiment studies from 2019-03-11 to 2019-04-23 (NCEI Accession 0306213). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/8kgb-wb45. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0). SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) Copeman, L. A., Stowell, M. A., Andrade, J. F., & Hurst, T. P. (2025). Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. Journal of Experimental Marine Biology and Ecology, 589, 152111. https://doi.org/10.1016/j.jembe.2025.152111 2025-08 publication International DOI Foundation (IDF) https://doi.org/10.1016/j.jembe.2025.152111 https://doi.org/10.1016/j.jembe.2025.152111 HTTPS https://doi.org/10.1016/j.jembe.2025.152111 related journal article information Copeman, Louise A. author Stowell, Michelle A. author Andrade, Jessica F. author Hurst, Thomas P. author Elsevier BV publisher documentDigital Journal of Experimental Marine Biology and Ecology 589 ISSN 0022-0981, p. 152111 crossReference project NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Global Ocean Carbon Data 2022 publication https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/ HTTPS https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/ Related resource information NOAA National Centers for Environmental Information (NCEI) publisher documentDigital crossReference project Slesinger, Emily; Copeman, Louise A.; Laurel, Benjamin J.; Hicks, Mary Beth R.; Iseri, Paul J.; Stowell, Michelle A.; Hurst, Thomas P. (2025). Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2 in laboratory experiment studies from 2023-03-09 to 2023-05-15 (NCEI Accession 0306528). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/thwf-6s28. 2025-07-24 publication International DOI Foundation (IDF) https://doi.org/10.25921/thwf-6s28 NOAA National Centers for Environmental Information gov.noaa.nodc:0306528 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306528.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306528.html Related dataset information Slesinger, Emily NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Copeman, Louise A. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Laurel, Benjamin J. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Hicks, Mary Beth R. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Iseri, Paul J. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Stowell, Michelle A. Cooperative Institute for Marine Ecosystem and Resource Studies https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Hurst, Thomas P. NOAA Alaska Fisheries Science Center http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project eng; USA utf8 environment oceans biota -124.043955 -124.043955 44.621316 44.621316 2019-03-11 2019-04-23 This data package (Submission ID: BJCEMAGNL) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Originator data format 2.38 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306213.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://doi.org/10.25921/8kgb-wb45 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/306213 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/306213 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0233/0306213/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0306213 v1.1 was published. 2025-07-16T16:16:39Z NCEI Accession 0306213 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0306213/1.1 HTTPS NCEI Accession 0306213 v1.1 published 2025-07-16T16:16:39Z download nonGeographicDataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer); Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: μmol/kg; Observation type: Labor; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity); CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pH; Abbreviation: calcpH; pH scale: SEAWATERSCALE(SWS); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 5.9±0.4°C; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: calcCO2; Unit: µatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Sampling date; Abbreviation: Date; Unit: none Parameter or Variable: Time of day of sample collection; Abbreviation: Time; Unit: none Parameter or Variable: Seawater sample identification; Abbreviation: Bottle_ID; Unit: none Parameter or Variable: Life stage of organism; Abbreviation: Life_stage; Unit: none; Detailed sampling and analyzing information: Coded as “LARVAL” Parameter or Variable: CO2 treatment; Abbreviation: CO2_trt; Unit: none; Detailed sampling and analyzing information: Coded as "Ambient" or "High" Parameter or Variable: Water temperature; Abbreviation: Temp; Unit: °C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Water salinity; Abbreviation: Salinity; Unit: ppt; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Analyzing instrument: Yellow Springs International meter - Model YSI 85; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Dissolved oxygen concentration in sea water; Abbreviation: DO; Unit: mg/L; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Analyzing instrument: Yellow Springs International meter - Model YSI 85 Units: mg/L; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Aragonite saturation state; Abbreviation: calcO Ar; Unit: omega; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: Calculated using CO2sys program; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Natalie Monacci; Researcher institution: Researcher Institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Data quality flag for CO2 system measures; Abbreviation: h2oDQ; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded: 1=good; 2=questionable/outlier/estimated; 3=known error; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Laboratory tank identifier; Abbreviation: tank; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Larval age in days post hatch when data were collected; Abbreviation: DPH; Unit: none; Observation type: Laboratory experiment Parameter or Variable: CO2 treatment; Abbreviation: CO2; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded as "Ambient" or "High" Parameter or Variable: Rotifer enrichment identification; Abbreviation: Diet_enrichment; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded as "Plus" for rotifers cultured on RotiGrow® Plus or "Nanno" for rotifers cultured on RotiGreen Nanno® (Reed Mariculture, Campbell, CA) Parameter or Variable: Diet type identification; Abbreviation: Diets_Type; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded as "Balanced" for rotifers cultured on RotiGrow® Plus (a blend of five different microalgae) or "Unbalanced" for rotifers cultured on RotiGreen Nanno® (a single-species medium of Nannochloropsis) (Reed Mariculture, Campbell, CA). Parameter or Variable: Count of larvae; Abbreviation: count; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: The number of larvae used to calculate the means ± standard deviations of each treatment replicate for standard length and dry weight Parameter or Variable: mean standard length; Abbreviation: mean_SL; Unit: mm; Observation type: Laboratory experiment; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. At each sampling interval, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean standard length; Abbreviation: SL_sd; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. At each sampling interval, up to 15 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 15 fish were measured from each treatment replicate on each sampling date; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: mean dry weight; Abbreviation: mean_DWT; Unit: µg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Sartorius Microbalance R16OP; Detailed sampling and analyzing information: Larvae were rinsed with 3% ammonium formate, dried for 24 hours at 50°C, and weighed on a microbalance (Sartorius R16OP) to the nearest microgram.; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: standard deviation of mean dry weight; Abbreviation: DWT_sd; Unit: µg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Analyzing instrument: Sartorius Microbalance R16OP; Detailed sampling and analyzing information: Larvae were rinsed with 3% ammonium formate, dried for 24 hours at 50°C, and weighed on a microbalance (Sartorius R16OP) to the nearest microgram.; Replicate information: Fish were weighed in pooled samples of up to 5 larvae and up to 3 times per tank and sampling event; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Lipid sample identification; Abbreviation: Lipid Sample ID; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Experiment identification (Ocean Acidification); Abbreviation: Experiment; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Sample type identification (rock sole); Abbreviation: Sample Type; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Larval age in weeks (post hatch); Abbreviation: Age_weeks; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Percentage of total lipids composed of triacylglycerols; Abbreviation: Perc_TAG; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a triacylglycerol standard purified from gadid liver; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of free fatty acids; Abbreviation: Perc_FFA; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of sterols; Abbreviation: Perc_ST; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of polar lipids; Abbreviation: Perc_PL; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Triacylglycerol amount per dry weight of lipid sample; Abbreviation: DWT_TAG; Unit: μg/mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Triacylglycerol amount (μg) divided by an estimate of the lipid sample dry weight (mg); Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Free fatty acid amount per dry weight of lipid sample; Abbreviation: DWT_FFA; Unit: μg/mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Free fatty acid amount (μg) divided by an estimate of the lipid sample dry weight (mg); Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Sterol amount per dry weight of lipid sample; Abbreviation: DWT_ST; Unit: μg/mg; Observation type: Laboratory experiment; Calculation method and parameters: Sterol amount (μg) divided by an estimate of the lipid sample dry weight (mg); Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Polar lipid amount per dry weight of lipid sample; Abbreviation: DWT_PL; Unit: μg/mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Polar lipid amount (μg) divided by an estimate of the lipid sample dry weight (mg); Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Total lipid amount per dry weight of lipid sample; Abbreviation: DWT_Total; Unit: μg/mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Sum of all lipid class amounts in the lipid sample (TAG, FFA, ST, PL, in μg) divided by an estimate of the lipid sample dry weight (mg); Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Triacylglycerol amount per individual larva; Abbreviation: per_larvae_TAG; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Triacylglycerol amount (μg) divided by number of larvae in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Free fatty acid amount per individual larva; Abbreviation: per_larvae_FFA; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Free fatty acid amount (μg) divided by number of larvae in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Sterol amount per individual larva; Abbreviation: per_larvae_ST; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Sterol amount (μg) divided by number of larvae in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Polar lipid amount per individual larva; Abbreviation: per_larvae_PL; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Polar lipid amount (μg) divided by number of larvae in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Total lipid amount per individual larva; Abbreviation: per_larvae_Total; Unit: μg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Total lipid amount (μg) divided by number of larvae in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Ratio of triacylglycerol to sterol in lipid sample; Abbreviation: TAG_ST; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Ratio of triacylglycerol amount (μg) to sterol amount (μg) in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories) with Peak Simple software (ver. 3.67, SRI, Inc.); Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Lipid sample identification; Abbreviation: Sample ID; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Diet type identification; Abbreviation: Diet; Unit: none; Observation type: Laboratory experiment; Detailed sampling and analyzing information: Coded as "Balanced" for rotifers cultured on RotiGrow® Plus (a blend of five different microalgae) or "Unbalanced" for rotifers cultured on RotiGreen Nanno® (a single-species medium of Nannochloropsis) (Reed Mariculture, Campbell, CA). Parameter or Variable: Larval age in weeks (post hatch); Abbreviation: Weeks; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Percentage (by weight) of total fatty acids composed of 14:0 (myristic acid) in lipid sample; Abbreviation: 14_0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 14:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 16:0 (palmitic acid) in lipid sample; Abbreviation: 16_0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Calculation method and parameters: Chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 16:1n-7 (palmitoleic acid) in lipid sample; Abbreviation: 16_1w7; Unit: none; Observation type: Lab; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 16:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:0 (stearic acid) in lipid sample; Abbreviation: 18_0; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 18:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:1n-9 (oleic acid) in lipid sample; Abbreviation: 18_1w9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 18:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:1n-7 (vaccenic acid) in lipid sample; Abbreviation: 18_1w7; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 18:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:2n-6 (linoleic acid) in lipid sample; Abbreviation: 18_2w6; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area for 18:2n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:3n-3 (alpha-linolenic acid) in lipid sample; Abbreviation: 18_3w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 18:3n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:1n-9 (eicosenoic acid) in lipid sample; Abbreviation: 20_1w9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:2n-6 (eicosadienoic acid) in lipid sample; Abbreviation: 20_2w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:2n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:4n-6 (arachidonic acid) in lipid sample; Abbreviation: 20_4w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:4n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:5n-3 (eicosapentaenoic acid) in lipid sample; Abbreviation: 20_5w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:5n-6 (osbond acid) in lipid sample; Abbreviation: 22_5w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 22:5n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:5n-3 (clupanodonic acid) in lipid sample; Abbreviation: 22_5w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 22:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:6n-3 (docosahexaenoic acid) in lipid sample; Abbreviation: 22_6w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 22:6n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Copeman LA, Stowell MA, Andrade JF, Hurst TP (2025) Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae. J Exp Mar Biol Ecol 589:152111. https://doi.org/10.1016/j.jembe.2025.152111; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian carbon dioxide (CO2) gas analyzer carbon dioxide (CO2) gas analyzer measures gaseous carbon dioxide Carbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption. Carbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water. To measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water. chromatograph chromatograph Gas, ion, liquid or other chromatograph oxygen sensor oxygen sensor Any instrument that measures oxygen content in a compound. pH sensor pH sensor generic meter used to measure pH Any of a class of instruments used to quantify pH in a water or other type of sample. temperature sensor temperature sensor A sensor used to measure temperature.