gov.noaa.nodc:0156588 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2025-04-03T22:18:09Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588) 2016-10-20 publication 2016-09-14 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0156588 NCEI Archive Management System 0156588 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Alexandra Simpson Western Washington University (WWU) alliegsimpson@gmail.com resourceProvider Western Washington University (WWU) resourceProvider David Shull principalInvestigator Sylvia Yang Western Washington University (WWU) collaborator Michael Adamczyk collaborator Phillip Papacjik collaborator Ari Blatt collaborator Dan Woodrich collaborator Miles Mayer collaborator Kate Ades collaborator Victoria Monreal collaborator Melissa Ciesielski collaborator Tyler Tran collaborator Laura Tripp collaborator Alexandra Simpson Western Washington University (WWU) alliegsimpson@gmail.com collaborator Adamczyk, Michael author Monreal, Victoria author Tran, Tyler author Yang, Sylvia author Papacjik, Phillip author Blatt, Ari author Woodrich, Dan author Mayer, Miles author Ades, Kate author Ciesielski, Melissa author Tripp, Laura author Simpson, Alexandra Western Washington University (WWU) alliegsimpson@gmail.com author Shull, David author Western Washington University (WWU) contributor tableDigital Multiple experiments were conducted to determine the effects hydrogen sulfide had on seedlings and seed germination in the seagrass Zostera marina. One study identified lethal limits of H2S for Z. marina seedlings, as well as identified ranges in which Z. marina seedlings are unaffected by sulfide levels. It was determined that Z. marina seedlings’ growth rates are stunted by H2S levels. Another study identified if anoxia, salinity, sulfide, time the seeds were picked or location the seeds were picked from affected seed germination rates or acted as germination cues. Germination rates for each treatment at each measurement were calculated by dividing the number of germinated seeds by the number of total seeds in each vial at each timepoint. A third seed study identified the best storage conditions for Z. marina seeds to maximize seed viability and germination rates, as well as identified possible germination cues for Z. marina seeds. Various seed storage conditions included fridge storage vs sea table storage, storage in mud vs sandy sediment vs seawater, light vs dark conditions and anoxic vs oxygenated seawater. The objectives of a fourth experiment were to census the density of Z. marina shoots along a transect in Padilla bay. By manipulating sediment conditions among some of the plots, we measured how organic matter in sediment is converted to sulfide and how that sulfide affects eelgrass shoot density. To do so blocks of agar and 100g of sucrose were prepared and each treatment plot received half of an agar block, placed beneath the sediment, every two weeks. Control plots had the sediment manipulated in the same way but without adding organic matter under the sediment. At each two week interval, pore water was sipped and measured for salinity, pH, and total sulfide content at each plot, the sediment temperature of each plot was taken, and the number of vegetative and flowering shoots at each plot was recorded. This dataset is available to the public for a wide variety of uses including scientific research and analysis. Funding Agency: Western Washington University completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0156588 Preview graphic PNG 0156588 NCEI ACCESSION NUMBER 2016-09-07 publication Hydrogen Sulfide (H2S) pH SALINITY TEMPERATURE - SEDIMENT theme NODC DATA TYPES THESAURUS laboratory analysis instrument NODC INSTRUMENT TYPES THESAURUS in situ laboratory analyses theme NODC OBSERVATION TYPES THESAURUS Western Washington University dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS Western Washington University dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS North Pacific Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication Eelgrass-Sulfide Project project Provider Project Names EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Plant Viability theme Provider Keywords Glass thermometer ROSS pH electrode Thermoscientific Orion Star A214 pH/ISE meter Vista Series Instruments A366ATC portable refractometer instrument Provider Instruments OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 24 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Padilla Bay, WA Samish Bay, WA Ship Harbor, WA Willapa Bay, WA place Provider Location Keywords 7C214R Send2NCEI Submission Package ID accessLevel: public otherRestrictions Cite as: Adamczyk, Michael; Monreal, Victoria; Tran, Tyler; Yang, Sylvia; Papacjik, Phillip; Blatt, Ari; Woodrich, Dan; Mayer, Miles; Ades, Kate; Ciesielski, Melissa; Tripp, Laura; Simpson, Alexandra; Shull, David (2016). Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0156588. Accessed [date]. 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However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Originator data format 0.476 https://www.ncei.noaa.gov/archive/accession/0156588 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/156588 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/156588 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0098/0156588/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0156588 v1.1 was published. 2016-10-20T14:28:43Z NCEI Accession 0156588 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0156588/1.1 HTTPS NCEI Accession 0156588 v1.1 published 2016-10-20T14:28:43Z download dataset Data Type: Hydrogen Sulfide (H2S) (measured); Units: millimole; Observation Type: laboratory analysis; Sampling Instrument: Thermoscientific Orion Star A214 pH/ISE meter; Sampling and Analyzing Method: Pore water sippers: capillary tubing with a syringe needle cap attached to one end with marine sealant. A hot needle was used to poke rows of small holes in the plastic cap. A septa was placed on the other end of the capillary tubing. The capillary tubing was marked with tape 3 cm above the holes that were poked. By pushing the tube, holes first into the sediment to the tape, we were able to extract pore water from a 3cm depth, with a needle and syringe through the septa, without oxygenating the pore water. Pore water is then mixed with a sulfide antioxidant buffer and measured with an ISE probe Data Type: Plant Viability (measured); Units: count; Observation Type: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Length of shoots, width of shoots, number of leaves, number of branches, seed germination, shoot color, density, flowering and vegetative shoot counts. Seeds which had a radicle fully extending from the seed (only attached to the seed at the base of the radicle) were considered germinated. At the end of the experiment, seeds were taken out of each vial with forceps. If the seed squished apart it was deemed unviable. If a shoot was present on germinated seed, a determination of shoot color was made Data Type: TEMPERATURE - SEDIMENT (measured); Units: C; Observation Type: in situ; Sampling Instrument: Glass thermometer Data Type: pH (measured); Units: pH; Observation Type: laboratory analysis; Sampling Instrument: ROSS pH electrode Data Type: SALINITY (measured); Units: ppt; Observation Type: laboratory analysis; Sampling Instrument: Vista Series Instruments A366ATC portable refractometer asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian laboratory analysis laboratory analysis use as an observation type