{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Timothy Fegel", "hasEmail": "mailto:tfegel@fs.fed.us"}, "description": "Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The \u201cCounts\u201d tab is the number of cells counted in each view of the microscope using the acridine orange method. \u201cC Calculations\u201d tab is the calculation of carbon as bacterial biomass from cell counts. \u201dChange in cell counts\u201d is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, \u201cCarbon use efficiency\u201d is calculated as the amount of DOM from oxygen demand assays converted to microbial biomass as carbon. The final two tabs \u201cBGE with calculated RQ\u201d and \u201cBGE with RQ1\u201d are the calculations of bacterial growth efficiency. The first uses a respiratory quotient calculated by using the change in CO2/the change in O2 from the biological oxygen demand assay measurements, and the second is calculated assuming a respiratory quotient of 1, a common practice in freshwater metabolism studies.\nDissolved organic matter date is Metabolite Compounds as Identified through Gas Chromatography - Mass Spectrometry (GC-MS) analyses. The \u201cGC-MS Data\u201d tab is the intensities of each compound separated out by individual glacier and rock glacier. The second tab is a table of the top 25 compounds in each glacier and rock glacier, as identified through GC-MS, before and after the biological oxygen demand assays. Included in this table is the fold changes in the mass spectrometry intensities of individual compounds. The third tab is the statistical comparison of each glacier type before and after incubation. \u201cRetention times\u201d, the fourth tab, is the time of detection of each individual compound on the mass spectrometer. Finally, the last tab is a raw data file of all spectra generated through GC-MS including mass spec contaminates, which were removed prior to the metabolite analysis.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P9A0JNP9", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5ca27c37e4b0b8a7f63172d1.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_5ca27c37e4b0b8a7f63172d1", "keyword": ["Colorado", "Indian Peaks", "Rocky Mountain", "USGS:5ca27c37e4b0b8a7f63172d1", "bacteria", "biogeochemical cycling", "biota", "carbon cycling", "dissolved organic compounds", "snow and ice cover"], "modified": "2022-07-14T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-105.7736, 39.9308, -105.5374, 40.3675", "theme": ["geospatial"], "title": "Laboratory Incubation results  from 2015 for bacterial cell counts, carbon use efficiency, growth efficiency, and dissolved organic matter chemistry from four glacier outflows and four rock glacier outflows in Colorado"}