{"@type": "dcat:Dataset", "DOI": "10.15121/1452718", "accessLevel": "public", "bureauCode": ["019:20"], "contactPoint": {"@type": "vcard:Contact", "fn": "Yongqin Jiao", "hasEmail": "mailto:jiao1@llnl.gov"}, "dataQuality": true, "description": "LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. \n\nFollowing adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://gdr.openei.org/files/965/GTO_data_deposit_092017_Desorption.xlsx", "description": "Excel file detailing results of the bicarbonate and citrate desorption experiments. Plots of results included.", "format": "xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "title": "Desorption Data.xlsx"}], "identifier": "https://data.openei.org/submissions/7133", "issued": "2017-06-01T06:00:00Z", "keyword": ["REE", "adsorption", "bicarbonate", "brine", "centrifuge", "citrate", "desorption", "energy", "geothermal", "lanthanide binding tag", "lbt", "rare earth", "resource recovery"], "landingPage": "https://gdr.openei.org/submissions/965", "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2018-06-14T16:28:49Z", "programCode": ["019:006"], "projectLead": "Holly Thomas", "projectNumber": "LLNL FY17 AOP 2.5.1.12", "projectTitle": "Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes", "publisher": {"@type": "org:Organization", "name": "Lawrence Livermore National Laboratory"}, "spatial": "{\"type\":\"Polygon\",\"coordinates\":[[[-122.2433640625,35.15607354867],[-118.3872640625,35.15607354867],[-118.3872640625,39.295415290912],[-122.2433640625,39.295415290912],[-122.2433640625,35.15607354867]]]}", "title": "Rare Earth Desorption Test with Citrate and Bicarbonate"}