gov.noaa.nodc:0306528 eng utf8 nonGeographicDataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-03-26T08:48:37Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2 in laboratory experiment studies from 2023-03-09 to 2023-05-15 (NCEI Accession 0306528) 2025-07-24 publication 2025-07-24 creation International DOI Foundation (IDF) 10.25921/thwf-6s28 NOAA National Centers for Environmental Information gov.noaa.nodc:0306528 NCEI Archive Management System 0306528 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Mara L. Spencer NOAA Alaska Fisheries Science Center (NMFS/AFSC) Mara.Spencer@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information resourceProvider Emily Slesinger NOAA Alaska Fisheries Science Center (NMFS/AFSC) emily.slesinger@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Louise Copeman NOAA Alaska Fisheries Science Center (NMFS/AFSC) louise.copeman@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Benjamin Laurel NOAA Alaska Fisheries Science Center (NMFS/AFSC) ben.laurel@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Mary Beth Rew Hicks NOAA Alaska Fisheries Science Center (NMFS/AFSC) marybeth.rew.hicks@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Paul J. Iseri NOAA Alaska Fisheries Science Center (NMFS/AFSC) paul.iseri@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Michelle Stowell Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information principalInvestigator Thomas Hurst NOAA Alaska Fisheries Science Center (NMFS/AFSC) Thomas.Hurst@noaa.gov http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information principalInvestigator Slesinger, Emily NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Copeman, Louise A. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Laurel, Benjamin J. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Hicks, Mary Beth R. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Iseri, Paul J. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author Stowell, Michelle A. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Hurst, Thomas P. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information contributor Oregon State University (OSU) http://oregonstate.edu/ HTTP Oregon State University (OSU) website Institution web page information contributor tableDigital This dataset contains laboratory experiment data that were collected to examine the effects of temperature and elevated pCO2 on the developmental and physiological responses of larval Arctic cod (Boregogadus saida). High-latitude ecosystems are simultaneously warming and acidifying under ongoing climate change. Arctic cod are a key species in the Arctic Ocean and have demonstrated sensitivity to ocean warming and acidification as adults and embryos, but their larval sensitivity to the combined stressors is unknown. In a laboratory multi-stressor experiment, larval Arctic cod were exposed to a combination of three temperatures (1.8, 5 and 7.3°C) and two carbon dioxide (pCO2) levels (ambient: 330 μatm, high: 1470 μatm) from hatching to 6-weeks of growth. Mortality rates were highest at 7.3°C (5% day-1); however, both growth and morphometric-based condition were also highest at this temperature. When these metrics were assessed via a mortality: growth (M:G) ratio, 5°C appeared to be an optimal temperature for net population biomass, as faster growth at 7.3°C did not fully compensate for higher mortality. In contrast, although morphometric-based condition was lowest at 1.8°C, lipid-based condition was highest, which may reflect prioritization of lipid storage at cold temperatures. The capacity of larval Arctic cod to acclimate to a range of temperatures was exhibited by two lipid-based indicators of membrane fluidity, including a ratio of unsaturated to saturated fatty acids and a ratio of polar lipids to sterols. The effects of elevated pCO2 were subtle, as well as temperature- and metric dependent. When exposed to elevated pCO2 levels, Arctic cod at 1.8°C exhibited signs of lipid dysregulation, suggesting potential interference with membrane acclimation; larvae at 5°C were in lower morphometric-based condition; and larvae at 7.3°C had higher activity eicosanoid substrates, indicating possible physiological stress. Overall, Arctic cod physiological response to temperature variation was more pronounced than their response to elevated pCO2. Future projections of pCO2 effects on Arctic cod health in a warming ecosystem will need to consider the complexity of temperature-dependence and the specificity of multiple physiological responses. To examine the effects of temperature and elevated pCO2 on the developmental and physiological responses of larval Arctic cod (Boregogadus saida). Funding Information: NOAA's Ocean Acidification Program (20903) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0306528 Preview graphic PNG 0306528 NCEI ACCESSION NUMBER 2025-07-23 publication DISSOLVED INORGANIC CARBON (DIC) DISSOLVED OXYGEN lipids mortality rate partial pressure of carbon dioxide - water pH SALINITY SPECIES IDENTIFICATION - DRY WEIGHT total alkalinity TOTAL DRY MASS WATER TEMPERATURE theme NODC DATA TYPES THESAURUS carbon dioxide (CO2) gas analyzer chromatograph conductivity sensor oxygen sensor temperature sensor instrument NODC INSTRUMENT TYPES THESAURUS biological chemical laboratory experiments physical survey - biological theme NODC OBSERVATION TYPES THESAURUS NOAA Alaska Fisheries Science Center Oregon State University dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Alaska Fisheries Science Center dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS North Pacific Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. 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URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Laboratory experiment theme OCADS Study Type 14:00 16:00 16:1w7 18:00 18:1w7 18:1w9 18:2w6 20:1w9 20:2w6 20:4w6 20:5w3 22:5w3 22:5w6 22:6w3 CO2.Treat DATE DIC DO DPH DW.m DW.se FFA KDW.m KDW.se KMH.m KMH.se LEN.m LEN.se MH.m MH.se MORT MUFA PUFA Polar_lip SFA ST Salinity TA TAG TAG:ST Tank Temp Temp.Treat n pCO2 pH.Meter pH.calc theme Provider Variable Abbreviations CO2 ANALYZERS > CO2 ANALYZERS CONDUCTIVITY METERS > CONDUCTIVITY METERS GAS CHROMATOGRAPHS > GAS CHROMATOGRAPHS OXYGEN METERS > OXYGEN METERS TEMPERATURE SENSORS > TEMPERATURE SENSORS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NOAA AFSC Laboratory Newport Oregon place Provider Geographic Names accessLevel: public otherRestrictions Cite as: Slesinger, Emily; Copeman, Louise A.; Laurel, Benjamin J.; Hicks, Mary Beth R.; Iseri, Paul J.; Stowell, Michelle A.; Hurst, Thomas P. (2025). Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2 in laboratory experiment studies from 2023-03-09 to 2023-05-15 (NCEI Accession 0306528). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/thwf-6s28. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0). SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) Slesinger, E., Copeman, L. A., Laurel, B. J., Hicks, M. B. R., Iseri, P. J., Stowell, M. A., & Hurst, T. P. (2025). Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. Journal of Fish Biology. https://doi.org/10.1111/jfb.70082 2025-06-05 publication International DOI Foundation (IDF) https://doi.org/10.1111/jfb.70082 https://doi.org/10.1111/jfb.70082 HTTPS https://doi.org/10.1111/jfb.70082 related journal article information Slesinger, Emily National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Copeman, Louise A. National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Laurel, Benjamin J. National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Hicks, Mary Beth R. National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Iseri, Paul J. National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Stowell, Michelle A. Cooperative Institute for Marine and Ecosystem Resources Studies Oregon State University Newport Oregon USA author Hurst, Thomas P. National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Hatfield Marine Science Center Newport Oregon USA author Wiley publisher documentDigital Journal of Fish Biology ISSN 0022-1112, ISSN 1095-8649 crossReference project NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Copeman, Louise A.; Stowell, Michelle A.; Andrade, Jessica F.; Hurst, Thomas P. (2025). Impacts of ocean acidification and altered prey fatty acids on the early development of northern rock sole (Lepidopsetta polyxystra) larvae from laboratory experiment studies from 2019-03-11 to 2019-04-23 (NCEI Accession 0306213). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/8kgb-wb45. 2025-07-16 publication International DOI Foundation (IDF) https://doi.org/10.25921/8kgb-wb45 NOAA National Centers for Environmental Information gov.noaa.nodc:0306213 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306213.html HTTPS https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306213.html Related dataset information Copeman, Louise A. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Stowell, Michelle A. Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) https://cimers.oregonstate.edu/ HTTPS Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website Institution web page information author Andrade, Jessica F. Lynker Technologies author Hurst, Thomas P. NOAA Alaska Fisheries Science Center (NMFS/AFSC) http://www.afsc.noaa.gov HTTP NOAA Alaska Fisheries Science Center (NMFS/AFSC) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference project Global Ocean Carbon Data 2022 publication https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/ HTTPS https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/ Related resource information NOAA National Centers for Environmental Information (NCEI) publisher documentDigital crossReference project eng; USA utf8 environment oceans biota -124.043955 -124.043955 44.621316 44.621316 2023-03-09 2023-05-15 This data package (Submission ID: BJEJSMMM8) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. 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Originator data format 0.152 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0306528.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://doi.org/10.25921/thwf-6s28 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/306528 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/306528 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0237/0306528/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0306528 v1.1 was published. 2025-07-24T23:16:09Z NCEI Accession 0306528 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0306528/1.1 HTTPS NCEI Accession 0306528 v1.1 published 2025-07-24T23:16:09Z download nonGeographicDataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer); Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: μmol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity); CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: saturated HgCl2; Preservative correction: no correction; Uncertainty: <0.05%; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pH; Abbreviation: pH.calc; pH scale: SEAWATERSCALE(SWS); Observation type: Lab; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 5.9±0.4°C; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: µatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: CALCULATED; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Natalie Monacci; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks Parameter or Variable: Sampling date; Abbreviation: DATE; Unit: none Parameter or Variable: CO2 treatment; Abbreviation: CO2.Treat; Unit: none; Detailed sampling and analyzing information: Coded as "amb" for ambient level or "high" for high treatment level. A Durafet III pH probe (Honeywell) in each of the high pCO2 treatment header tanks regulated the injection of CO2 based on achieving temperature-dependent pH targets (see Slesinger et al., 2024). Parameter or Variable: Water temperature treatment designation; Abbreviation: Temp.Treat; Unit: °C; Detailed sampling and analyzing information: Coded as “egg inc” for data taken during egg incubation prior to the start of the experiment, “egg start” for data taken at the start of the experiment, and “1.8”, “5”, or “7.3” for the targeted treatment temperatures during the experiment. Parameter or Variable: pH recorded from meter; Abbreviation: pH.Meter; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Analyzing instrument: Durafet III pH probe (Honeywell); Detailed sampling and analyzing information: Measured in experimental tank with Honeywell Durafet III meter at the time of collection of the water sample collected for determination of TA and DIC (see Slesinger et al., 2024).; Method reference: Slesinger, E., Mundorff, S., Laurel, B. J., & Hurst, T. P. (2024). The combined effects of ocean warming and ocean acidificaiton on Pacific cod (Gadus macrocephalus) early life stages. Marine Biology, 171, 121; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Water temperature; Abbreviation: Temp; Unit: °C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Water salinity; Abbreviation: Salinity; Unit: ppt; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Analyzing instrument: Yellow Springs International meter - Model YSI 85; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Dissolved oxygen concentration in sea water; Abbreviation: DO; Unit: mg/L; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: MANIPULATIONCONDITION; Measured or calculated: MEASURED; Analyzing instrument: Yellow Springs International meter - Model YSI 85; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Larval age in days post hatch; Abbreviation: DPH; Unit: days; Observation type: Laboratory experiment Parameter or Variable: Laboratory tank identifier; Abbreviation: Tank; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Sample size; Abbreviation: n; Unit: none; Observation type: Laboratory experiment Parameter or Variable: Mean standard length; Abbreviation: LEN.m; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (SL; mm) and myotome height at the anus (MH; mm).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard error of mean standard length; Abbreviation: LEN.se; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (SL; mm) and myotome height at the anus (MH; mm).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Mean dry weight; Abbreviation: DW.m; Unit: mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Detailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard error of mean dry weight; Abbreviation: DW.se; Unit: mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Detailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Mean myotome height; Abbreviation: MH.m; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (SL; mm) and myotome height at the anus (MH; mm).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard error of mean myotome height; Abbreviation: MH.se; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (SL; mm) and myotome height at the anus (MH; mm).; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Mean dry weight-based condition; Abbreviation: KDW.m; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Detailed sampling and analyzing information: Morphometric-based condition metrics were calculated based on the residuals of the relationship between SL and MH for body depth-based condition (KMH), and SL and DW for dry weight-based condition (KDW). KMH was defined as the difference between the expected to measured MH at a specific SL based on a cubic fit. KDW was defined as the quotient of the expected and measured DW for a specific SL based on a quadratic fit.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard error of mean dry weight-based condition; Abbreviation: KDW.se; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Detailed sampling and analyzing information: Morphometric-based condition metrics were calculated based on the residuals of the relationship between SL and MH for body depth-based condition (KMH), and SL and DW for dry weight-based condition (KDW). KMH was defined as the difference between the expected to measured MH at a specific SL based on a cubic fit. KDW was defined as the quotient of the expected and measured DW for a specific SL based on a quadratic fit.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Mean of body depth-based condition; Abbreviation: KMH.m; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Morphometric-based condition metrics were calculated based on the residuals of the relationship between SL and MH for body depth-based condition (KMH), and SL and DW for dry weight-based condition (KDW). KMH was defined as the difference between the expected to measured MH at a specific SL based on a cubic fit. KDW was defined as the quotient of the expected and measured DW for a specific SL based on a quadratic fit.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Standard error of mean of body depth-based condition; Abbreviation: KMH.se; Unit: mm; Observation type: Laboratory experiment; Sampling instrument: dissecting microscope; Analyzing instrument: ImageJ software; Detailed sampling and analyzing information: Morphometric-based condition metrics were calculated based on the residuals of the relationship between SL and MH for body depth-based condition (KMH), and SL and DW for dry weight-based condition (KDW). KMH was defined as the difference between the expected to measured MH at a specific SL based on a cubic fit. KDW was defined as the quotient of the expected and measured DW for a specific SL based on a quadratic fit.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Mortality rate; Abbreviation: MORT; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Detailed sampling and analyzing information: At the end of the experiment, remaining larvae were counted and removed from the tanks to provide an estimate for mortality rates across the 6-week span.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Emily Slesinger; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of triacylglycerols; Abbreviation: TAG; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a triacylglycerol standard purified from gadid liver; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories); Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of free fatty acids; Abbreviation: FFA; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories); Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of sterols; Abbreviation: ST; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories); Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage of total lipids composed of polar lipids; Abbreviation: Polar_lip; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area was used with a calibration curve based on a commercial (Sigma) standard; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories); Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Ratio of triacylglycerol to sterol in lipid sample; Abbreviation: TAG:ST; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Ratio of triacylglycerol amount (μg) to sterol amount (μg) in lipid sample; Analyzing instrument: MARK VI Iatroscan (Iatron Laboratories); Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Copeman LA, Laurel BJ, Spencer M, Sremba A (2017) Temperature impacts on lipid allocation among juvenile gadid species at the Pacific Arctic-Boreal interface: an experimental laboratory approach. Mar Ecol Prog Ser 566:183-198. https://doi.org/10.3354/meps12040; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 14:0 (myristic acid) in lipid sample; Abbreviation: 14:00; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 14:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 16:0 (palmitic acid) in lipid sample; Abbreviation: 16:00; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 16:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 16:1n-7 (palmitoleic acid) in lipid sample; Abbreviation: 16:1w7; Unit: none; Observation type: Labo; Calculation method and parameters: Chromatogram peak area for 16:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:0 (stearic acid) in lipid sample; Abbreviation: 18:00; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area for 18:0 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:1n-9 (oleic acid) in lipid sample; Abbreviation: 18:1w9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 18:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:1n-7 (vaccenic acid) in lipid sample; Abbreviation: 18:1w7; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area for 18:1n-7 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 18:2n-6 (linoleic acid) in lipid sample; Abbreviation: 18:2w6; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area for 18:2n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:1n-9 (eicosenoic acid) in lipid sample; Abbreviation: 20:1w9; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:1n-9 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:2n-6 (eicosadienoic acid) in lipid sample; Abbreviation: 20:2w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:2n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:4n-6 (arachidonic acid) in lipid sample; Abbreviation: 20:4w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:4n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 20:5n-3 (eicosapentaenoic acid) in lipid sample; Abbreviation: 20:5w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 20:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:5n-6 (osbond acid) in lipid sample; Abbreviation: 22:5w6; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 22:5n-6 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:5n-3 (clupanodonic acid) in lipid sample; Abbreviation: 22:5w3; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Chromatogram peak area for 22:5n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of 22:6n-3 (docosahexaenoic acid) in lipid sample; Abbreviation: 22:6w3; Unit: none; Observation type: Laboratory experiment; Calculation method and parameters: Chromatogram peak area for 22:6n-3 divided by the sum of all chromatogram peak areas and expressed as a percentage; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Slesinger E, Copeman LA, Laurel BJ, Hicks MBR, Iseri PJ, Stowell MA, Hurst TP (2025) Larval Arctic cod (Boreogadus saida) exhibit stronger developmental and physiological responses to temperature than to elevated pCO2. J Fish Biol 2025:1-15. https://doi.org/10.1111/jfb.70082; Method reference: Budge SM, Iverson SJ, Koopman HN (2006) Studying trophic ecology in marine ecosystems using fatty acids: a primer on analysis and interpretation. Mar Mammal Sci 22:759-801. https://doi.org/10.1111/j.1748-7692.2006.00079.x; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of saturated fatty acids in lipid sample; Abbreviation: SFA; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Sum of percentages of all saturated fatty acids in the lipid sample; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Fatty acids were classified based on their carbon structure into SFAs, MUFAs and PUFAs.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of monounsaturated fatty acids in lipid sample; Abbreviation: MUFA; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Sum of percentages of all monounsaturated fatty acids in the lipid sample; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Fatty acids were classified based on their carbon structure into SFAs, MUFAs and PUFAs.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center Parameter or Variable: Percentage (by weight) of total fatty acids composed of polyunsaturated fatty acids in lipid sample; Abbreviation: PUFA; Unit: none; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: RESPONSEVARIABLE; Measured or calculated: MEASURED; Calculation method and parameters: Sum of percentages of all polyunsaturated fatty acids in the lipid sample; Sampling instrument: Gas chromatograph; Analyzing instrument: HP 7890 GCFID; Detailed sampling and analyzing information: Fatty acids were classified based on their carbon structure into SFAs, MUFAs and PUFAs.; Biological subject: Arctic cod; Species ID: Boreogadus saida; Researcher name: Louise A. Copeman; Researcher institution: NOAA Alaska Fisheries Science Center asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian carbon dioxide (CO2) gas analyzer carbon dioxide (CO2) gas analyzer measures gaseous carbon dioxide Carbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption. Carbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water. To measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water. chromatograph chromatograph Gas, ion, liquid or other chromatograph conductivity sensor conductivity sensor OMEGA non-contact conductivity sensor CDCN-108 or Honeywell DirectLine DL423 Sensor Module, for example Water conductivity sensors are used to measure how well a solution conducts an electrical current. oxygen sensor oxygen sensor Any instrument that measures oxygen content in a compound. temperature sensor temperature sensor A sensor used to measure temperature.