{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Alaa Kamel", "hasEmail": "mailto:kamel.alaa@epa.gov"}, "description": "Three chemicals, 2-ethylhexyl 4-hydroxybenzoate (2-EHHB), 5-chloro-2-(2,4-dichlorophenoxy) phenol (triclosan), and 4-nonylphenol, branched (4-NP) were evaluated using the Larval Amphibian Growth and Development Assay (LAGDA) to investigate endocrine-mediated effects. A delay in development to NF stage 62 was observed for the three chemicals, however, histopathological evaluation revealed that the effects were likely not thyroid related. 4-NP caused increased stage scores in male and female gonadal duct development. Citation information for this dataset can be found in Data.gov's References section.", "distribution": [{"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1532344/Larval%20Amphibian%20Growth%20and%20Development%20Assay%20%28LAGDA%29%20of%202-Ethylhexyl%204-Hydroxybenzoate%20.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "Larval Amphibian Growth and Development Assay (LAGDA) of 2-Ethylhexyl 4-Hydroxybenzoate .docx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1532344/Larval%20Amphibian%20Growth%20and%20Development%20Assay%20%28LAGDA%29%20of%204-nonylphenol.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "Larval Amphibian Growth and Development Assay (LAGDA) of 4-nonylphenol.docx"}, {"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1532344/Larval%20Amphibian%20Growth%20and%20Development%20Assay%20%28LAGDA%29%20of%20Triclosan.docx", "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document", "title": "Larval Amphibian Growth and Development Assay (LAGDA) of Triclosan.docx"}], "identifier": "https://doi.org/10.23719/1532344", "keyword": ["2-Ethylhexyl 4-Hydroxybenzoate", "4-nonylphenol", "LAGDA", "Triclosan", "Xenopus", "amphibian", "endocrine disruption"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2025-06-09", "programCode": ["020:000"], "publisher": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}, "references": ["https://pasteur.epa.gov/uploads/10.23719/1532344/documents/Supplemental%20Spreadsheet%20S1%20a-c.xlsx"], "rights": null, "title": "Evaluation of the potential effects of three chemicals on the hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-gonadal (HPG) axes using the Larval Amphibian Growth and Development Assay (LAGDA) "}