{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "David S. Pilliod", "hasEmail": "mailto:dpilliod@usgs.gov"}, "description": "This dataset contains environmental DNA (eDNA) data for two separate tests of a novel quantitative polymerase chain reaction (qPCR) assay for detection of Dakota skipper (Hesperia dacotae) from inflorescences. One test was conducted in a controlled setting, and the other was conducted in a field setting. Also included are qPCR data for negative controls and standard dilutions of Dakota skipper gBlock.", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P13JBGIU", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.688bbecfd4be025d5d20f72f.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_688bbecfd4be025d5d20f72f", "keyword": ["Minnesota", "North Dakota", "South Dakota", "USGS:688bbecfd4be025d5d20f72f", "Upper Midwest", "biota", "environment", "environmental DNA", "genetics", "location"], "modified": "2025-11-26T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-104.0625, 43.4529, -91.0547, 48.9514", "theme": ["geospatial"], "title": "Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025)"}