{
  "accessLevel": "public",
  "bureauCode": [
    "020:00"
  ],
  "contactPoint": {
    "fn": "Jay Reichman",
    "hasEmail": "mailto:reichman.jay@epa.gov"
  },
  "description": "Fecal samples from migratory birds were collected from contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected.\n\nCO1 primers with Illumina adaptors:\nCO1-F\tTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAACATTCAGCCATCTTACCCGTG\nCO1-R\tGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACAGGGAGTGATAGRAGKAGTAGG\n\nAlso, chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds, which can be found at DOI: 10.23719/1532393",
  "distribution": [
    {
      "accessURL": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348",
      "title": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348"
    },
    {
      "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1532394/Bird_CO1_SRA_metadata_Final.xlsx",
      "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
      "title": "Bird_CO1_SRA_metadata_Final.xlsx"
    }
  ],
  "identifier": "https://doi.org/10.23719/1532394",
  "keyword": [
    "Amplicon-sequencing",
    "Birds",
    "CO1",
    "Pb toxicity"
  ],
  "license": "https://pasteur.epa.gov/license/sciencehub-license.html",
  "modified": "2025-06-26",
  "programCode": [
    "020:000"
  ],
  "publisher": {
    "name": "U.S. EPA Office of Research and Development (ORD)",
    "subOrganizationOf": {
      "name": "U.S. Environmental Protection Agency",
      "subOrganizationOf": {
        "name": "U.S. Government"
      }
    }
  },
  "references": null,
  "rights": null,
  "title": "Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: CO1 dataset"
}